Sequence of pGEM-Sox2

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Sequence of pGEM-Sox2

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Sequence of pGEM-Sox2 : 3983 BP; 976 A; 1076 C; 1075 G; 856 T; 0 other;

.......... AatII(20) .......... .......... .......... ..........

1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 60

1 cccgcttaac ccgggctgca gcgtacgagg gccggcggta ccgccggcgc ccttaagcta 60

.......... .......... .......... ......... Orf frame 3 .........

.......... .......... .......... .......... .......... ..........

61 tccgcatgta caacatgatg gagacggagc tgaagccgcc gggcccgcag caaacttcgg 120

61 aggcgtacat gttgtactac ctctgcctcg acttcggcgg cccgggcgtc gtttgaagcc 120

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggggcggcgg cggcaactcc accgcggcgg cggccggcgg caaccagaaa aacagcccgg 180

121 ccccgccgcc gccgttgagg tggcgccgcc gccggccgcc gttggtcttt ttgtcgggcc 180

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 accgcgtcaa gcggcccatg aatgccttca tggtgtggtc ccgcgggcag cggcgcaaga 240

181 tggcgcagtt cgccgggtac ttacggaagt accacaccag ggcgcccgtc gccgcgttct 240

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tggcccagga gaaccccaag atgcacaact cggagatcag caagcgcctg ggcgccgagt 300

241 accgggtcct cttggggttc tacgtgttga gcctctagtc gttcgcggac ccgcggctca 300

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggaaactttt gtcggagacg gagaagcggc cgttcatcga cgaggctaag cggctgcgag 360

301 cctttgaaaa cagcctctgc ctcttcgccg gcaagtagct gctccgattc gccgacgctc 360

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cgctgcacat gaaggagcac ccggattata aataccggcc ccggcggaaa accaagacgc 420

361 gcgacgtgta cttcctcgtg ggcctaatat ttatggccgg ggccgccttt tggttctgcg 420

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcatgaagaa ggataagtac acgctgcccg gcgggctgct ggcccccggc ggcaatagca 480

421 agtacttctt cctattcatg tgcgacgggc cgcccgacga ccgggggccg ccgttatcgt 480

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tggcgagcgg ggtcggggtg ggcgccggcc tgggcgcggg cgtgaaccag cgcatggaca 540

481 accgctcgcc ccagccccac ccgcggccgg acccgcgccc gcacttggtc gcgtacctgt 540

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gttacgcgca catgaacggc tggagcaacg gcagctacag catgatgcag gaccagctgg 600

541 caatgcgcgt gtacttgccg acctcgttgc cgtcgatgtc gtactacgtc ctggtcgacc 600

.......... ......... Orf frame 3 ......... .......... ..........

.......... SmaI(616) .......... .......... .......... ..........

601 gctacccgca gcacccgggc ctcaatgcgc acggcgcagc gcagatgcag cccatgcacc 660

601 cgatgggcgt cgtgggcccg gagttacgcg tgccgcgtcg cgtctacgtc gggtacgtgg 660

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gctacgacgt gagcgccctg cagtacaact ccatgaccag ctcgcagacc tacatgaacg 720

661 cgatgctgca ctcgcgggac gtcatgttga ggtactggtc gagcgtctgg atgtacttgc 720

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gctcgcccac ctacagcatg tcctactcgc agcagggcac ccctggcatg gctcttggct 780

721 cgagcgggtg gatgtcgtac aggatgagcg tcgtcccgtg gggaccgtac cgagaaccga 780

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccatgggttc ggtggtcaag tccgaggcca gctccagccc ccctgtggtt acctcttcct 840

781 ggtacccaag ccaccagttc aggctccggt cgaggtcggg gggacaccaa tggagaagga 840

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... BclI(883) ..........

841 cccactccag ggcgccctgc caggccgggg acctccggga catgatcagc atgtatctcc 900

841 gggtgaggtc ccgcgggacg gtccggcccc tggaggccct gtactagtcg tacatagagg 900

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccggcgccga ggtgccggaa cccgccgccc ccagcagact tcacatgtcc cagcactacc 960

901 ggccgcggct ccacggcctt gggcggcggg ggtcgtctga agtgtacagg gtcgtgatgg 960

.......... ......... Orf frame 3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agagcggccc ggtgcccggc acggccatta acggcacact gcccctctca cacatgtgag 1020

961 tctcgccggg ccacgggccg tgccggtaat tgccgtgtga cggggagagt gtgtacactc 1020

.......... ......... Orf frame 3 ......... .......... ..........

.......... SpeI(1032) .......... SalI(1058) NdeI(1065) SacI(1077)

1021 ggccggaaat cactagtgaa ttcgcggccg cctgcaggtc gaccatatgg gagagctccc 1080

1021 ccggccttta gtgatcactt aagcgccggc ggacgtccag ctggtatacc ctctcgaggg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aacgcgttgg atgcatagct tgagtattct atagtgtcac ctaaatagct tggcgtaatc 1140

1081 ttgcgcaacc tacgtatcga actcataaga tatcacagtg gatttatcga accgcattag 1140

.......... .......... .......... Sp6_primer .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 1200

1141 taccagtatc gacaaaggac acactttaac aataggcgag tgttaaggtg tgttgtatgc 1200

M13_reverse_primer M13_pUC_rev_primer .... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 1260

1201 tcggccttcg tatttcacat ttcggacccc acggattact cactcgattg agtgtaatta 1260

......... lac_promoter ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 1320

1261 acgcaacgcg agtgacgggc gaaaggtcag ccctttggac agcacggtcg acgtaattac 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 1380

1321 ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc gcgagaaggc gaaggagcga 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 1440

1381 gtgactgagc gacgcgagcc agcaagccga cgccgctcgc catagtcgag tgagtttccg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 1500

1441 ccattatgcc aataggtgtc ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 1560

1501 ggtcgttttc cggtccttgg catttttccg gcgcaacgac cgcaaaaagg tatccgaggc 1560

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1620

1561 ggggggactg ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt tgggctgtcc 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1680

1621 tgatatttct atggtccgca aagggggacc ttcgagggag cacgcgagag gacaaggctg 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1740

1681 ggacggcgaa tggcctatgg acaggcggaa agagggaagc ccttcgcacc gcgaaagagt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1800

1741 atcgagtgcg acatccatag agtcaagcca catccagcaa gcgaggttcg acccgacaca 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1860

1801 cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg ccattgatag cagaactcag 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1920

1861 gttgggccat tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1980

1921 tcgctccata catccgccac gatgtctcaa gaacttcacc accggattga tgccgatgtg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 2040

1981 atcttcttgt cataaaccat agacgcgaga cgacttcggt caatggaagc ctttttctca 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 2100

2041 accatcgaga actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 2160

2101 cgtcgtctaa tgcgcgtctt tttttcctag agttcttcta ggaaactaga aaagatgccc 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 2220

2161 cagactgcga gtcaccttgc ttttgagtgc aattccctaa aaccagtact ctaatagttt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 2280

2221 ttcctagaag tggatctagg aaaatttaat ttttacttca aaatttagtt agatttcata 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 2340

2281 tatactcatt tgaaccagac tgtcaatggt tacgaattag tcactccgtg gatagagtcg 2340

.......... .......... .......... .... Orf frame 1 Ampicillin ....

.......... .......... .......... .......... .......... ..........

2341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 2400

2341 ctagacagat aaagcaagta ggtatcaacg gactgagggg cagcacatct attgatgcta 2400

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 2460

2401 tgccctcccg aatggtagac cggggtcacg acgttactat ggcgctctgg gtgcgagtgg 2460

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 2520

2461 ccgaggtcta aatagtcgtt atttggtcgg tcggccttcc cggctcgcgt cttcaccagg 2520

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 2580

2521 acgttgaaat aggcggaggt aggtcagata attaacaacg gcccttcgat ctcattcatc 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 2640

2581 aagcggtcaa ttatcaaacg cgttgcaaca acggtaacga tgtccgtagc accacagtgc 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 2700

2641 gagcagcaaa ccataccgaa gtaagtcgag gccaagggtt gctagttccg ctcaatgtac 2700

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 2760

2701 tagggggtac aacacgtttt ttcgccaatc gaggaagcca ggaggctagc aacagtcttc 2760

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 2820

2761 attcaaccgg cgtcacaata gtgagtacca ataccgtcgt gacgtattaa gagaatgaca 2820

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 2880

2821 gtacggtagg cattctacga aaagacactg accactcatg agttggttca gtaagactct 2880

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 2940

2881 tatcacatac gccgctggct caacgagaac gggccgcagt tatgccctat tatggcgcgg 2940

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 3000

2941 tgtatcgtct tgaaattttc acgagtagta accttttgca agaagccccg cttttgagag 3000

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 3060

3001 ttcctagaat ggcgacaact ctaggtcaag ctacattggg tgagcacgtg ggttgactag 3060

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 3120

3061 aagtcgtaga aaatgaaagt ggtcgcaaag acccactcgt ttttgtcctt ccgttttacg 3120

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 3180

3121 gcgttttttc ccttattccc gctgtgcctt tacaacttat gagtatgaga aggaaaaagt 3180

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 3240

3181 tataataact tcgtaaatag tcccaataac agagtactcg cctatgtata aacttacata 3240

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

3241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgatgc 3300

3241 aatcttttta tttgtttatc cccaaggcgc gtgtaaaggg gcttttcacg gtggactacg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt 3360

3301 ccacacttta tggcgtgtct acgcattcct cttttatggc gtagtccttt aacattcgca 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata 3420

3361 attataaaac aattttaagc gcaatttaaa aacaatttag tcgagtaaaa aattggttat 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 3480

3421 ccggctttag ccgttttagg gaatatttag ttttcttatc tggctctatc ccaactcaca 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 3540

3481 acaaggtcaa accttgttct caggtgataa tttcttgcac ctgaggttgc agtttcccgc 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 3600

3541 tttttggcag atagtcccgc taccgggtga tgcacttggt agtgggatta gttcaaaaaa 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 3660

3601 ccccagctcc acggcatttc gtgatttagc cttgggattt ccctcggggg ctaaatctcg 3660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 3720

3661 aactgcccct ttcggccgct tgcaccgctc tttccttccc ttctttcgct ttcctcgccc 3720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 3780

3721 gcgatcccgc gaccgttcac atcgccagtg cgacgcgcat tggtggtgtg ggcggcgcga 3780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg 3840

3781 attacgcggc gatgtcccgc gcaggtaagc ggtaagtccg acgcgttgac aacccttccc 3840

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

3841 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 3900

3841 gctagccacg cccggagaag cgataatgcg gtcgaccgct ttccccctac acgacgttcc 3900

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 3960

3901 gctaattcaa cccattgcgg tcccaaaagg gtcagtgctg caacattttg ctgccggtca 3960

.......... ...... lacZ_a M13_pUC_fwd_primer . M13_forward20_primer

.......... .......... .......... .......... .......... ..........

3961 gaattgtaat acgactcact ata....... .......... .......... .......... 3983

3961 cttaacatta tgctgagtga tat....... .......... .......... .......... 3983

.......... .......... .......... .......... .......... ..........