Sequence of pGEX-5X-1

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Sequence of pGEX-5X-1

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Sequence of pGEX-5X-1 : 4972 BP; 1228 A; 1201 C; 1293 G; 1250 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 60

1 tcgaatagct gacgtgccac gtggttacga agaccgcagt ccgtcggtag ccttcgacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 120

61 cataccgaca cgtccagcat ttagtgacgt attaagcaca gcgagttccg cgtgagggca 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 180

121 agacctatta caaaaaacgc ggctgtagta ttgccaagac cgtttataag actttactcg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 240

181 acaactgtta attagtagcc gagcatatta cacaccttaa cactcgccta ttgttaaagt 240

.......... tac_pro .......... .......... ....... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 300

241 gtgtcctttg tcataagtac aggggatatg atccaataac cttttaattc ccggaacacg 300

.......... .......... .......... ORF 3 GST .......... ..........

.......... .......... .......... .......... .......... ..........

301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 360

301 ttgggtgagc tgaagaaaac cttatagaac ttctttttat acttctcgta aacatactcg 360

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 420

361 cgctacttcc actatttacc gctttgtttt tcaaacttaa cccaaacctc aaagggttag 420

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... MscI(465) ..........

421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 480

421 aaggaataat ataactacca ctacaattta attgtgtcag ataccggtag tatgcaatat 480

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 540

481 atcgactgtt cgtgttgtac aacccaccaa caggttttct cgcacgtctc taaagttacg 540

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 600

541 aacttcctcg ccaaaaccta taatctatgc cacaaagctc ttaacgtata tcatttctga 600

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... BstBI(655)

601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 660

601 aactttgaga gtttcaacta aaagaatcgt tcgatggact ttacgacttt tacaagcttc 660

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 720

661 tagcaaatac agtattttgt ataaatttac cactagtaca ttgggtagga ctgaagtaca 720

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 780

721 acatactgcg agaactacaa caaaatatgt acctgggtta cacggaccta cgcaagggtt 780

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 840

781 ttaatcaaac aaaatttttt gcataacttc gatagggtgt ttaactattc atgaacttta 840

.......... .......... ORF 3 GST .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 900

841 ggtcgttcat atatcgtacc ggaaacgtcc cgaccgttcg gtgcaaacca ccaccgctgg 900

.......... .......... ORF 3 GST pGEX_5_pri .......... ..........

.......... .......... .......... BamHI(934) EcoRI(942) ..........

901 atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgaattcccg ggtcgactcg 960

901 taggaggttt tagcctagac tagcttccag caccctaggg gcttaagggc ccagctgagc 960

.......... .......... ORF 3 GST .......... .......... ..........

NotI(963),EagI(963) .......... .......... .......... ..........

961 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa 1020

961 tcgccggcgt agcactgact gactgctaga cggagcgcgc aaagccacta ctgccacttt 1020

ORF 3 GST .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 1080

1021 tggagactgt gtacgtcgag ggcctctgcc agtgtcgaac agacattcgc ctacggccct 1080

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 1140

1081 cgtctgttcg ggcagtcccg cgcagtcgcc cacaaccgcc cacagccccg cgtcggtact 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac 1200

1141 gggtcagtgc atcgctatcg cctcacatat taagaacttc tgctttcccg gagcactatg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AatII(1248) .........

1201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 1260

1201 cggataaaaa tatccaatta cagtactatt attaccaaag aatctgcagt ccaccgtgaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 1320

1261 aagccccttt acacgcgcct tggggataaa caaataaaaa gatttatgta agtttataca 1320

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 1380

1321 taggcgagta ctctgttatt gggactattt acgaagttat tataactttt tccttctcat 1380

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 1440

1381 actcataagt tgtaaaggca cagcgggaat aagggaaaaa acgccgtaaa acggaaggac 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 1500

1441 aaaaacgagt gggtctttgc gaccactttc attttctacg acttctagtc aacccacgtg 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 1560

1501 ctcacccaat gtagcttgac ctagagttgt cgccattcta ggaactctca aaagcggggc 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 1620

1561 ttcttgcaaa aggttactac tcgtgaaaat ttcaagacga tacaccgcgc cataataggg 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 1680

1621 cacaactgcg gcccgttctc gttgagccag cggcgtatgt gataagagtc ttactgaacc 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 1740

1681 aactcatgag tggtcagtgt cttttcgtag aatgcctacc gtactgtcat tctcttaata 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 1800

1741 cgtcacgacg gtattggtac tcactattgt gacgccggtt gaatgaagac tgttgctagc 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 1860

1801 ctcctggctt cctcgattgg cgaaaaaacg tgttgtaccc cctagtacat tgagcggaac 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 1920

1861 tagcaaccct tggcctcgac ttacttcggt atggtttgct gctcgcactg tggtgctacg 1920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

PstI(1925) .......... .......... .......... .......... ..........

1921 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 1980

1921 gacgtcgtta ccgttgttgc aacgcgtttg ataattgacc gcttgatgaa tgagatcgaa 1980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 2040

1981 gggccgttgt taattatctg acctacctcc gcctatttca acgtcctggt gaagacgcga 2040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 2100

2041 gccgggaagg ccgaccgacc aaataacgac tatttagacc tcggccactc gcacccagag 2100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 2160

2101 cgccatagta acgtcgtgac cccggtctac cattcgggag ggcatagcat caatagatgt 2160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 2220

2161 gctgcccctc agtccgttga tacctacttg ctttatctgt ctagcgactc tatccacgga 2220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 2280

2221 gtgactaatt cgtaaccatt gacagtctgg ttcaaatgag tatatatgaa atctaactaa 2280

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 2340

2281 attttgaagt aaaaattaaa ttttcctaga tccacttcta ggaaaaacta ttagagtact 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 2400

2341 ggttttaggg aattgcactc aaaagcaagg tgactcgcag tctggggcat cttttctagt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 2460

2401 ttcctagaag aactctagga aaaaaagacg cgcattagac gacgaacgtt tgtttttttg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 2520

2461 gtggcgatgg tcgccaccaa acaaacggcc tagttctcga tggttgagaa aaaggcttcc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 2580

2521 attgaccgaa gtcgtctcgc gtctatggtt tatgacagga agatcacatc ggcatcaatc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 2640

2581 cggtggtgaa gttcttgaga catcgtggcg gatgtatgga gcgagacgat taggacaatg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 2700

2641 gtcaccgacg acggtcaccg ctattcagca cagaatggcc caacctgagt tctgctatca 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 2760

2701 atggcctatt ccgcgtcgcc agcccgactt gccccccaag cacgtgtgtc gggtcgaacc 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 2820

2761 tcgcttgctg gatgtggctt gactctatgg atgtcgcact cgatactctt tcgcggtgcg 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 2880

2821 aagggcttcc ctctttccgc ctgtccatag gccattcgcc gtcccagcct tgtcctctcg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 2940

2881 cgtgctccct cgaaggtccc cctttgcgga ccatagaaat atcaggacag cccaaagcgg 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 3000

2941 tggagactga actcgcagct aaaaacacta cgagcagtcc ccccgcctcg gatacctttt 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 3060

3001 tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac gaccggaaaa cgagtgtaca 3060

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 3120

3061 agaaaggacg caatagggga ctaagacacc tattggcata atggcggaaa ctcactcgac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 3180

3121 tatggcgagc ggcgtcggct tgctggctcg cgtcgctcag tcactcgctc cttcgccttc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt 3240

3181 tcgcggacta cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg gcgtatttaa 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 3300

3241 ggctgtggta gcttaccacg ttttggaaag cgccataccg tactatcgcg ggccttctct 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 3360

3301 cagttaagtc ccaccactta cactttggtc attgcaatat gctacagcgt ctcatacggc 3360

.......... .......... .......... lacI .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 3420

3361 cacagagaat agtctggcaa agggcgcacc acttggtccg gtcggtgcaa agacgctttt 3420

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 3480

3421 gcgccctttt tcaccttcgc cgctaccgcc tcgacttaat gtaagggttg gcgcaccgtg 3480

.......... .......... lacI .......... ORF 3 ..........

.......... .......... .......... .......... .......... ..........

3481 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 3540

3481 ttgttgaccg cccgtttgtc agcaacgact aaccgcaacg gtggaggtca gaccgggacg 3540

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 3600

3541 tgcgcggcag cgtttaacag cgccgctaat ttagagcgcg gctagttgac ccacggtcgc 3600

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 3660

3601 accaccacag ctaccatctt gcttcgccgc agcttcggac atttcgccgc cacgtgttag 3660

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 3720

3661 aagagcgcgt tgcgcagtca cccgactagt aattgatagg cgacctactg gtcctacggt 3720

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 3780

3721 aacgacacct tcgacggacg tgattacaag gccgcaataa agaactacag agactggtct 3780

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 3840

3781 gtgggtagtt gtcataataa aagagggtac ttctgccatg cgctgacccg cacctcgtag 3840

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... ApaI(3882) ..........

3841 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 3900

3841 accagcgtaa cccagtggtc gtttagcgcg acaatcgccc gggtaattca agacagagcc 3900

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 3960

3901 gcgcagacgc agaccgaccg accgtattta tagagtgagc gttagtttaa gtcggctatc 3960

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 4020

3961 gccttgccct tccgctgacc tcacggtaca ggccaaaagt tgtttggtac gtttacgact 4020

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 4080

4021 tactcccgta gcaagggtga cgctacgacc aacggttgct agtctaccgc gacccgcgtt 4080

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 4140

4081 acgcgcggta atggctcagg cccgacgcgc aaccacgcct atagagccat caccctatgc 4140

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... HpaI(4177) .......... ..........

4141 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 4200

4141 tgctatggct tctgtcgagt acaatatagg gcggcaattg gtggtagttt gtcctaaaag 4200

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 4260

4201 cggacgaccc cgtttggtcg cacctggcga acgacgttga gagagtcccg gtccgccact 4260

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... NarI(4311)

4261 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 4320

4261 tcccgttagt cgacaacggg cagagtgacc acttttcttt ttggtgggac cgcgggttat 4320

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 4380

4321 gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt gctgtccaaa 4380

.......... .......... lacI ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 4440

4381 gggctgacct ttcgcccgtc actcgcgttg cgttaattac actcaatcga gtgagtaatc 4440

.......... lacI ORF 3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 4500

4441 cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca acacacctta acactcgcct 4500

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt 4560

4501 attgttaaag tgtgtccttt gtcgatactg gtactaatgc ctaagtgacc ggcagcaaaa 4560

M13_pUC_rev_pri . M13_reverse_pri ....... ... lacZ_a M13_forward20_pri

.......... .......... .......... .......... .......... ..........

4561 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 4620

4561 tgttgcagca ctgacccttt tgggaccgca atgggttgaa ttagcggaac gtcgtgtagg 4620

M13_pUC_fwd_pri .... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 4680

4621 gggaaagcgg tcgaccgcat tatcgcttct ccgggcgtgg ctagcgggaa gggttgtcaa 4680

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga 4740

4681 cgcgtcggac ttaccgctta ccgcgaaacg gaccaaaggc cgtggtcttc gccacggcct 4740

lacZ_a .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca 4800

4741 ttcgaccgac ctcacgctag aaggactccg gctatgacag cagcagggga gtttgaccgt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc 4860

4801 ctacgtgcca atgctacgcg ggtagatgtg gttgcattgg atagggtaat gccagttagg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga 4920

4861 cggcaaacaa gggtgcctct taggctgccc aacaatgagc gagtgtaaat tacaactact 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt........ 4972

4921 ttcgaccgat gtccttccgg tctgcgctta ataaaaacta ccgcaacctt aa........ 4972

.......... .......... .......... .......... .......... ..........