LL-LOCUS pGFPTA 3249 bp DNA SYN 12-Oct-2007 DEFINITION pGFPTA ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3249 /organism="pGFPTA" /mol_type="other DNA" promoter 88..117 /label="lac_promoter" misc_feature 131..153 /label="M13_pUC_rev_primer" misc_feature 152..170 /label="M13_reverse_primer" misc_feature complement(264..292) /label="GFP_R_primer" misc_feature complement(308..327) /label="GFPfusionREV_primer" CDS 162..950 /label="GFP_cyc3" /gene="GFP_cyc3" /note="ORF frame 3" /translation="MTMITPSLHACRSTLEDPRVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM KRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVL LPDNHYLSTQSALLKDPNEKRDHMVLLEFVTAAGITHGMDELYK*" gene 234..950 /label="GFP_cyc3" /gene="GFP_cyc3" misc_feature 887..907 /label="GFP_F_primer" promoter 1093..1121 /label="AmpR_promoter" CDS 1163..2023 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1163..2023 /label="Ampicillin" /gene="Ampicillin" rep_origin 2178..2797 /label="pBR322_origin" misc_feature 3194..3216 /label="pGEX_3_primer" ORIGIN 1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 181 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accggtagaa aaaatgagta 241 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 301 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 361 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 421 ctctgacgta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact 481 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cacgatatct ttcaaagatg 541 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta 601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt 661 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag 721 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc 781 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga 841 cacaatctgc ccttttgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt 901 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa tgaattcttg 961 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 1021 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 1081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 1141 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 1201 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 1261 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 1321 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 1381 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 1441 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 1501 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 1561 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 1621 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1681 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac 1741 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1801 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1861 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1921 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1981 acagatcgct gagataggtg cgtcactgat taagcattgg taactgtcag accaagttta 2041 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 2101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 2341 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 2581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 2821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 2881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 2941 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg 3001 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 3061 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 3121 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 3181 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 3241 gcgaggcag // pGFPTA "Times New RomanLC Xulac_pro"Times New RomanLC M13_pUC_rev_pri"Times New RomanLCɆ M13_reverse_pri"Times New RomanLC ORF 3"Times New RomanLCɆ GFP_cyc3"Times New RomanL $ GFP_R_pri"Times New Roman*LC 4GGFPfusionREV_pri"Times New Roman*L w GFP_F_pri"Times New Roman*LC EaAmpR_pro"Times New RomanLC ORF 2"Times New RomanLCɆ  Ampicillin"Times New RomanL   pBR322_origin"Times New RomanLC z  pGEX_3_pri"Times New Roman L4HindIII(179),XbaI(203),SmaI(216),AgeI(221),KpnI(222)"Times New Roman u*bu LNcoI(399),MscI(404),EcoRV(526)"Times New Roman X_ee LXhoI"Times New Roman  L SacI(940),EcoRI(952),AatII(1031)"Times New Roman  LFspI"Times New Roman GQZjZi LPvuII"Times New Roman  l Ltest"Times New Roman yS  { B