Sequence of pGFPTA

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pGFPTA

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pGFPTA : 3249 BP; 858 A; 774 C; 789 G; 828 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 60

1 gaccgtgctg tccaaagggc tgacctttcg cccgtcactc gcgttgcgtt aattacactc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 120

61 aatcgagtga gtaatccgtg gggtccgaaa tgtgaaatac gaaggccgag catacaacac 120

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 180

121 accttaacac tcgcctattg ttaaagtgtg tcctttgtcg atactggtac taatgcggtt 180

.......... ....... M13_pUC_rev_pri .... M13_reverse_pri ORF 3

.......... .......... XbaI(203) SmaI(216),AgeI(221),KpnI(222)

181 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accggtagaa aaaatgagta 240

181 cgaacgtacg gacgtccagc tgagatctcc taggggccca tggccatctt ttttactcat 240

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 300

241 ttcctcttct tgaaaagtga cctcaacagg gttaagaaca acttaatcta ccactacaat 300

.......... ........ ORF 3 GFP_cyc3 GFP_R_pri ........ ..........

.......... .......... .......... .......... .......... ..........

301 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 360

301 tacccgtgtt taaaagacag tcacctctcc cacttccact acgttgtatg ccttttgaat 360

..... GFPfusionREV_pri ORF 3 GFP_cyc3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 420

361 gggaatttaa ataaacgtga tgaccttttg atggacaagg taccggttgt gaacagtgat 420

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctctgacgta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact 480

421 gagactgcat accacaagtt acgaaaaggg caataggcct agtatacttt gccgtactga 480

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... EcoRV(526) ..........

481 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cacgatatct ttcaaagatg 540

481 aaaagttctc acggtacggg cttccaatac atgtccttgc gtgctataga aagtttctac 540

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta 600

541 tgcccttgat gttctgcgca cgacttcagt tcaaacttcc actatgggaa caattagcat 600

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... XhoI(654)

601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt 660

601 agctcaattt tccataacta aaatttcttc tacctttgta agagcctgtg tttgagctca 660

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag 720

661 tgttgatatt gagtgtgtta catatgtagt gccgtctgtt tgttttctta ccttagtttc 720

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc 780

721 gattgaagtt ttaagcggtg ttgtaacttc tacctaggca agttgatcgt ctggtaatag 780

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga 840

781 ttgttttatg aggttaaccg ctaccgggac aggaaaatgg tctgttggta atggacagct 840

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 cacaatctgc ccttttgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt 900

841 gtgttagacg ggaaaacttt ctagggttgc ttttcgcact ggtgtaccag gaagaactca 900

.......... ........ ORF 3 GFP_cyc3 ........ .......... GFP_F_pri

.......... .......... .......... SacI(940) .......... ..........

901 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa tgaattcttg 960

901 aacattgacg acgaccctaa tgtgtaccgt acctactcga gatgtttatt acttaagaac 960

.......... ........ ORF 3 GFP_cyc3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 1020

961 ttctgctttc ccggagcact atgcggataa aaatatccaa ttacagtact attattacca 1020

.......... .......... .......... .......... .......... ..........

......... AatII(1031) ......... .......... .......... ..........

1021 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 1080

1021 aagaatctgc agtccaccgt gaaaagcccc tttacacgcg ccttggggat aaacaaataa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 1140

1081 aaagatttat gtaagtttat acataggcga gtactctgtt attgggacta tttacgaagt 1140

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 1200

1141 tattataact ttttccttct catactcata agttgtaaag gcacagcggg aataagggaa 1200

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1201 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 1260

1201 aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt tgcgaccact ttcattttct 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 1320

1261 acgacttcta gtcaacccac gtgctcaccc aatgtagctt gacctagagt tgtcgccatt 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 1380

1321 ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 1440

1381 cgatacaccg cgccataata gggcacaact gcggcccgtt ctcgttgagc cagcggcgta 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 1500

1441 tgtgataaga gtcttactga accaactcat gagtggtcag tgtcttttcg tagaatgcct 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 1560

1501 accgtactgt cattctctta atacgtcacg acggtattgg tactcactat tgtgacgccg 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 1620

1561 gttgaatgaa gactgttgct agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1680

1621 ccccctagta cattgagcgg aactagcaac ccttggcctc gacttacttc ggtatggttt 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... FspI(1727) ..........

1681 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac 1740

1681 gctgctcgca ctgtggtgct acggacgtcg ttaccgttgt tgcaacgcgt ttgataattg 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1800

1741 accgcttgat gaatgagatc gaagggccgt tgttaattat ctgacctacc tccgcctatt 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1860

1801 tcaacgtcct ggtgaagacg cgagccggga aggccgaccg accaaataac gactatttag 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1920

1861 acctcggcca ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc taccattcgg 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1980

1921 gagggcatag catcaataga tgtgctgccc ctcagtccgt tgatacctac ttgctttatc 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acagatcgct gagataggtg cgtcactgat taagcattgg taactgtcag accaagttta 2040

1981 tgtctagcga ctctatccac gcagtgacta attcgtaacc attgacagtc tggttcaaat 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 2100

2041 gagtatatat gaaatctaac taaattttga agtaaaaatt aaattttcct agatccactt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 2160

2101 ctaggaaaaa ctattagagt actggtttta gggaattgca ctcaaaagca aggtgactcg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 2220

2161 cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag acgcgcatta 2220

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 2280

2221 gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg gcctagttct 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 2340

2281 cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg gtttatgaca 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 2400

2341 ggaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg gcggatgtat 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 2460

2401 ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca gcacagaatg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 2520

2461 gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga cttgcccccc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 2580

2521 aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta tggatgtcgc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 2640

2581 actcgatact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca taggccattc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 2700

2641 gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 2760

2701 aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca ctacgagcag 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 2820

2761 tccccccgcc tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca aggaccggaa 2820

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 2880

2821 aacgaccgga aaacgagtgt acaagaaagg acgcaatagg ggactaagac acctattggc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 2940

2881 ataatggcgg aaactcactc gactatggcg agcggcgtcg gcttgctggc tcgcgtcgct 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg 3000

2941 cagtcactcg ctccttcgcc ttctcgcgga ctacgccata aaagaggaat gcgtagacac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 3060

3001 gccataaagt gtggcgtata ccacgtgaga gtcatgttag acgagactac ggcgtatcaa 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 3120

3061 ttcggtcata tgtgaggcga tagcgatgca ctgacccagt accgacgcgg ggctgtgggc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 3180

3121 ggttgtgggc gactgcgcgg gactgcccga acagacgagg gccgtaggcg aatgtctgtt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 3240

3181 cgacactggc agaggccctc gacgtacaca gtctccaaaa gtggcagtag tggctttgcg 3240

.......... .......... pGEX_3_pri .......... .......... ..........

PvuII(3249) ........ .......... .......... .......... ..........

3241 gcgaggcag. .......... .......... .......... .......... .......... 3249

3241 cgctccgtc. .......... .......... .......... .......... .......... 3249

.......... .......... .......... .......... .......... ..........