Sequence of pGH8

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Sequence of pGH8

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Sequence of pGH8 - pRAB-3::mCherry::unc-54utr : 5340 BP; 1544 A; 1115 C; 1117 G; 1564 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtatagaaaa gttgatcttc agatgggagc agtggactgt ctatttgata ttcttaaaat 60

1 catatctttt caactagaag tctaccctcg tcacctgaca gataaactat aagaatttta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gtactctata aaagatgaaa ctgtaatgac caattcccta cataaatatt tttaactaat 120

61 catgagatat tttctacttt gacattactg gttaagggat gtatttataa aaattgatta 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tggtcggata tttggggatc agagaagtac ttgtttttgc gatattaata atgatatatt 180

121 accagcctat aaacccctag tctcttcatg aacaaaaacg ctataattat tactatataa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttctagtaa aaaaatgtta agcgatttgt gaataattaa gttggcttga aaaagatttt 240

181 aaagatcatt tttttacaat tcgctaaaca cttattaatt caaccgaact ttttctaaaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttgatagcta tgtattttgt taatttaaat cagattttaa aactttatta ttgactattt 300

241 aactatcgat acataaaaca attaaattta gtctaaaatt ttgaaataat aactgataaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atatatatta tatattatat attttcatgg tttaatcaaa atctattaaa ttatttctaa 360

301 tatatataat atataatata taaaagtacc aaattagttt tagataattt aataaagatt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aatctctaaa tatcgaattc tctaaaaagc ttcatcaaca aaagcatttc aattatctaa 420

361 ttagagattt atagcttaag agatttttcg aagtagttgt tttcgtaaag ttaatagatt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cacaatgaga ataattcaag aactgaaata gaaagaaatc tagaaaaaaa agagagtgag 480

421 gtgttactct tattaagttc ttgactttat ctttctttag atcttttttt tctctcactc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtgccctatt aaatcggtgc tctcctctct aacagaagcg cctacacgca cccaagagag 540

481 cacgggataa tttagccacg agaggagaga ttgtcttcgc ggatgtgcgt gggttctctc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctctttttct ttcttttttc ggagcacaca tacacgcata cactcctcag aagtgtatag 600

541 gagaaaaaga aagaaaaaag cctcgtgtgt atgtgcgtat gtgaggagtc ttcacatatc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 agaaaatggg agaagaagcg aaagaagacg aagaagcggt tgtgatgtat gcatcgggcg 660

601 tcttttaccc tcttcttcgc tttcttctgc ttcttcgcca acactacata cgtagcccgc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ClaI(700) .......... ..........

661 tttctatctc tctccgtgag caacgagcta gtcaacccat cgatccattt ttgtgagcac 720

661 aaagatagag agaggcactc gttgctcgat cagttgggta gctaggtaaa aacactcgtg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acacagagag agactcaaac acactcacag cttcacactg agctccaaga gctctgtaga 780

721 tgtgtctctc tctgagtttg tgtgagtgtc gaagtgtgac tcgaggttct cgagacatct 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagaagccag agtatgcttt cctcgatttt cctaacgtct tcttcttctg cttttttctt 840

781 ctcttcggtc tcatacgaaa ggagctaaaa ggattgcaga agaagaagac gaaaaaagaa 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tttcccaacg agttgtagtc cactaacttc aatttttcct ccagagaaag agttttagag 900

841 aaagggttgc tcaacatcag gtgattgaag ttaaaaagga ggtctctttc tcaaaatctc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agacagctga taaatacact agagaaataa aaagaatgat aaaaatttgc tattttaatc 960

901 tctgtcgact atttatgtga tctctttatt tttcttacta tttttaaacg ataaaattag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agttccctct cgtttctcta agttttgttg ttgggttggg aaattaaaat tggaaaatgg 1020

961 tcaagggaga gcaaagagat tcaaaacaac aacccaaccc tttaatttta accttttacc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaataacgtt tttttctgtc taatcttgaa ttctccagac atttcttatt tccttttttg 1080

1021 tttattgcaa aaaaagacag attagaactt aagaggtctg taaagaataa aggaaaaaac 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttgcaagttt ttcttccttc tctaattttt aatcaaagat atctcgctat gaactttgaa 1140

1081 aacgttcaaa aagaaggaag agattaaaaa ttagtttcta tagagcgata cttgaaactt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tttttgatat tttgattcta taaatacata aagtaatcta aatgctcttt taaaataaat 1200

1141 aaaaactata aaactaagat atttatgtat ttcattagat ttacgagaaa attttattta 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctacagtagc cctattttca gatgacaagt ttgtacaaaa aagcaggctt aatggtctca 1260

1201 gatgtcatcg ggataaaagt ctactgttca aacatgtttt ttcgtccgaa ttaccagagt 1260

.......... .......... .......... attB1 ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1261 aagggtgaag aagataacat ggcaattatt aaagagttta tgcgtttcaa ggtgcatatg 1320

1261 ttcccacttc ttctattgta ccgttaataa tttctcaaat acgcaaagtt ccacgtatac 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gagggatctg tcaatgggca tgagtttgaa attgaaggtg aaggagaagg ccgaccatat 1380

1321 ctccctagac agttacccgt actcaaactt taacttccac ttcctcttcc ggctggtata 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagggaacac aaaccgcaaa actaaaggta agtttaaaca tatatatact aactaaccct 1440

1381 ctcccttgtg tttggcgttt tgatttccat tcaaatttgt atatatatga ttgattggga 1440

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gattatttaa attttcaggt aactaaaggc ggaccattac cattcgcctg ggacatcctc 1500

1441 ctaataaatt taaaagtcca ttgatttccg cctggtaatg gtaagcggac cctgtaggag 1500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tctccacagt tcatgtatgg aagtaaagct tatgttaaac atccggcaga tataccagat 1560

1501 agaggtgtca agtacatacc ttcatttcga atacaatttg taggccgtct atatggtcta 1560

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tatttgaaac tttcattccc ggagggtttt aagtgggaac gcgtaatgaa ttttgaagac 1620

1561 ataaactttg aaagtaaggg cctcccaaaa ttcacccttg cgcattactt aaaacttctg 1620

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggaggagttg ttacagtgac gcaagactca aggtaagttt aaacagttcg gtactaacta 1680

1621 cctcctcaac aatgtcactg cgttctgagt tccattcaaa tttgtcaagc catgattgat 1680

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 accatacata tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt 1740

1681 tggtatgtat aaatttaaaa gtcggaggtt ctacctctta aataaatatt tcagtttgaa 1740

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgaggaacga atttcccctc ggatggacct gttatgcaga agaagactat gggatgggaa 1800

1741 gctccttgct taaaggggag cctacctgga caatacgtct tcttctgata ccctaccctt 1800

.......... .......... ORF 1 .......... .......... ..........

.......... .......... ......... AflII(1837) ......... ..........

1801 gcttcaagtg aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt 1860

1801 cgaagttcac tttcttacat gggacttctg ccacgagaat tccctctcta atttgttgca 1860

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cttaaattga aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta 1920

1861 gaatttaact ttctacctcc tgtaatgcta cgactccatt caaatttgta ctaaaatgat 1920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 actaactaat ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt 1980

1921 tgattgatta gactaaattt aaaagtccac ttctgttgaa tgtttcggtt ttttggtcaa 1980

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagctgccag gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat 2040

1981 gtcgacggtc ctcgcatgtt acaattataa tttgacctat agtggagggt gttgctccta 2040

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tacactatcg ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat 2100

2041 atgtgatagc aactcgttat actttctcga cttcccgccg tgagctgtcc accgtaccta 2100

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... EagI(2160)

2101 gaattgtata agtagtaccc agctttcttg tacaaagtgg tgatatctga gctccgcatc 2160

2101 cttaacatat tcatcatggg tcgaaagaac atgtttcacc actatagact cgaggcgtag 2160

ORF 1 .......... attB2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag tccaattact 2220

2161 ccggcgacag tagtctagcg gtagagcgcg ggcacggaga ctgaagattc aggttaatga 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc 2280

2221 gaagttgtag ggatgtacga gaaagaggga cacgagggtg ggggataaaa acaataatag 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa 2340

2281 ttttttgaag agaattaaag aaacaaaaaa tcgaagaaaa ttcagtggag attgttactt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc 2400

2341 taacacatct aagtttttat cttaattaag cattattttt cagctttttt taacacgagg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt 2460

2401 gaggggggta attattatta agatagggtt ttagatgtgt tacaagacac atgtgaagaa 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata 2520

2461 tacaaaaaat gaagactatt taaaaaaact ttgtagtatc ttttttggcg tgtgttttat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg 2580

2521 ggaatagtat acaatgcaaa gtcaaatact ggcgttaaaa ataaagaagc gtgcagaccc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt 2640

2581 ggagagtact gcagtttagt acgagtagca ctttttcaaa acctcataaa aaccttaaaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct 2700

2641 agttagttca ctttcaaata ctttaattaa aaggacgaaa acgaaaaacc ccaaagggga 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag 2760

2701 taacaaacag ttctaaagct cctgccgcaa aaagaacgat tttagtgttc ataactactc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga 2820

2761 gtgctacgtt ctttctagcc ttcttccaaa cccaaactcc gagtcacctt ccactcatct 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac 2880

2821 tcaactatta aactttcacc tcatcacaga taccccaaaa acggaattta ctgtcttatg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 attcccaata taccaaacat aactgtttcc aactttatta tacatagttg ataattcact 2940

2881 taagggttat atggtttgta ttgacaaagg ttgaaataat atgtatcaac tattaagtga 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct 3000

2941 ccggcagcaa aatgttgcag cactgaccct tttgggaccg caatgggttg aattagcgga 3000

M13_forward20_pri M13_pUC_fwd_pri lacZ_a ...... ..........

.......... .......... .......... .......... .......... ..........

3001 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc 3060

3001 acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt ggctagcggg 3060

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... NarI(3094) .......... ..........

3061 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac 3120

3061 aagggttgtc aacgcgtcgg acttaccgct taccgcggac tacgccataa aagaggaatg 3120

.......... lacZ_a .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 3180

3121 cgtagacacg ccataaagtg tggcgtatac cacgtgagag tcatgttaga cgagactacg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 3240

3181 gcgtatcaat tcggtcgggg ctgtgggcgg ttgtgggcga ctgcgcggga ctgcccgaac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca 3300

3241 agacgagggc cgtaggcgaa tgtctgttcg acactggcag aggccctcga cgtacacagt 3300

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3301 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt 3360

3301 ctccaaaagt ggcagtagtg gctttgcgcg ctctgctttc ccggagcact atgcggataa 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg 3420

3361 aaatatccaa ttacagtact attattacca aagaatctgc agtccaccgt gaaaagcccc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 3480

3421 tttacacgcg ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga 3480

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat 3540

3481 gtactctgtt attgggacta tttacgaagt tattataact ttttccttct catactcata 3540

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc 3600

3541 agttgtaaag gcacagcggg aataagggaa aaaacgccgt aaaacggaag gacaaaaacg 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg 3660

3601 agtgggtctt tgcgaccact ttcattttct acgacttcta gtcaacccac gtgctcaccc 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg 3720

3661 aatgtagctt gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga 3780

3721 aaaaggttac tactcgtgaa aatttcaaga cgatacaccg cgccataata gggcataact 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta 3840

3781 gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga gtcttactga accaactcat 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc 3900

3841 gagtggtcag tgtcttttcg tagaatgcct accgtactgt cattctctta atacgtcacg 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc 3960

3901 acggtattgg tactcactat tgtgacgccg gttgaatgaa gactgttgct agcctcctgg 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg 4020

3961 cttcctcgat tggcgaaaaa acgtgttgta ccccctagta cattgagcgg aactagcaac 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc 4080

4021 ccttggcctc gacttacttc ggtatggttt gctgctcgca ctgtggtgct acggacatcg 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca 4140

4081 ttaccgttgt tgcaacgcgt ttgataattg accgcttgat gaatgagatc gaagggccgt 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct 4200

4141 tgttaattat ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg cgagccggga 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat 4260

4201 aggccgaccg accaaataac gactatttag acctcggcca ctcgcaccca gagcgccata 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg 4320

4261 gtaacgtcgt gaccccggtc taccattcgg gagggcatag catcaataga tgtgctgccc 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat 4380

4321 ctcagtccgt tgatacctac ttgctttatc tgtctagcga ctctatccac ggagtgacta 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact 4440

4381 attcgtaacc attgacagtc tggttcaaat gagtatatat gaaatctaac taaattttga 4440

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat 4500

4441 agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4560

4501 gggaattgca ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag 4560

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 4620

4561 aagaactcta ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 4680

4621 tggtcgccac caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 4740

4681 gaagtcgtct cgcgtctatg gtttatgaca ggaagatcac atcggcatca atccggtggt 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 4800

4741 gaagttcttg agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 4860

4801 acgacggtca ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 4920

4861 attccgcgtc gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 4980

4921 ctggatgtgg cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 5040

4981 tccctctttc cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 5100

5041 cctcgaaggt ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 5160

5101 tgaactcgca gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 caacgcggcc tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc 5220

5161 gttgcgccgg aaaaatgcca aggaccagaa aacgaccgga aaacgagtgt acaagaaagg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag 5280

5221 acgcaatagg ggactaagac acctattggc ataatggcgg aaactcactc gactatggtc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatcaacttt 5340

5281 gcctattgtt aaagtgtgtc ctttgtcgat actggtacta atgcggttcg atagttgaaa 5340

.... M13_pUC_rev_pri M13_reverse_pri .... .......... ..........