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    Plasmid map of pGP-FB-orig_BA

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    pGP-FB-orig BA
    Gene/insert name: orig BA
    Alternative names: original BCR-ABL three-finger array
    Insert size (bp): 260
    Species of gene(s): Other
    Fusion proteins or tags: Gal11P
    Terminal: N terminal on backbone
    Vector backbone: pGP-FB (Search Vector Database)
    Type of vector: Bacterial expression
    Backbone size (bp): 3732
    Cloning site 5': XbaI
    Site destroyed during cloning: No
    Cloning site 3': BamHI
    Site destroyed during cloning: No
    5' Sequencing primer: GGG GGA TTT CTG TTC ATG GGG G (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: Low Copy
    Grow in standard E. coli @ 37C: No
    Bacterial strain for growth and growth condition: Needs strain with lacIq
    Plasmid Provided In: XL1 Blue
    Principal Investigator: Keith Joung
    Comments: Positive control for B2H beta-galactosidase assay. Has coding sequence for ?original? BCR-ABL three-finger array.
    This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.



    image of pGP-FB-orig_BA



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