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pGP-FB-orig BA
Gene/insert name: orig BA
Alternative names: original BCR-ABL three-finger array
Insert size (bp): 260
Species of gene(s): Other
Fusion proteins or tags: Gal11P
Terminal: N terminal on backbone
Vector backbone: pGP-FB (Search Vector Database)
Type of vector: Bacterial expression
Backbone size (bp): 3732
Cloning site 5': XbaI
Site destroyed during cloning: No
Cloning site 3': BamHI
Site destroyed during cloning: No
5' Sequencing primer: GGG GGA TTT CTG TTC ATG GGG G (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: No
Bacterial strain for growth and growth condition: Needs strain with lacIq
Plasmid Provided In: XL1 Blue
Principal Investigator: Keith Joung
Comments: Positive control for B2H beta-galactosidase assay. Has coding sequence for ?original? BCR-ABL three-finger array.
This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.