Sequence of pGP-FB-orig

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Sequence of pGP-FB-orig_BA

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Sequence of pGP-FB-orig BA : 3732 BP; 933 A; 949 C; 920 G; 930 T; 0 other;

EcoRI(1) .......... .......... .......... SalI(44) ..........

1 gaattccggg ctttacactt tatgcttccg gctcgtataa tgtgtcgact tgtgagcgga 60

1 cttaaggccc gaaatgtgaa atacgaaggc cgagcatatt acacagctga acactcgcct 60

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taacaatttc acacaggaaa cagctatgcc tcaacagcag caaatgcaac ctcccaattc 120

61 attgttaaag tgtgtccttt gtcgatacgg agttgtcgtc gtttacgttg gagggttaag 120

M13_pUC_rev_pri .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagtgcggac aacaaccctt tgcaacagca atcatcacaa aataccgtac caaacgtcct 180

121 ttcacgcctg ttgttgggaa acgttgtcgt tagtagtgtt ttatggcatg gtttgcagga 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaccaaatt aaccaaatct tttctccaga ggagcaacgc agcttattac aagaagccat 240

181 gttggtttaa ttggtttaga aaagaggtct cctcgttgcg tcgaataatg ttcttcggta 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cgaaacctgc aagaattttg aaaaaacaca attgggtagt acgatgacgg aacctgtcaa 300

241 gctttggacg ttcttaaaac ttttttgtgt taacccatca tgctactgcc ttggacagtt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... HindIII(351)

301 gcaaagtttt attaggaaat acattgtgca aaaggccctg agaaaaatcc aagctttggc 360

301 cgtttcaaaa taatccttta tgtaacacgt tttccgggac tctttttagg ttcgaaaccg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XbaI(392) .......... ..........

361 ggccgctgac tacaaagacg atgatgacaa atctagaccc ggggagcgcc ccttccagtg 420

361 ccggcgactg atgtttctgc tactactgtt tagatctggg cccctcgcgg ggaaggtcac 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcgcatttgc atgcggaact tttcggaccg atcctcgctt acgcgtcata cccgtactca 480

421 agcgtaaacg tacgccttga aaagcctggc taggagcgaa tgcgcagtat gggcatgagt 480

.......... .......... .......... .......... .......... ..........

AgeI(482) .......... .......... .......... .......... ..........

481 taccggtgaa aaaccgtttc agtgtcggat ctgtatgcga aatttctccc agggggggaa 540

481 atggccactt tttggcaaag tcacagccta gacatacgct ttaaagaggg tccccccctt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttggtgcgg catctacgta cgcacaccgg cgagaagcca ttccaatgcc gaatatgcat 600

541 aaaccacgcc gtagatgcat gcgtgtggcc gctcttcggt aaggttacgg cttatacgta 600

.......... .......... .......... .......... .......... ..........

.......... .......... PstI(630) .......... .......... BamHI(656)

601 gcgcaacttc agtcaggcag ccaccctgca gcgacaccta aaaacccacc tgaggggatc 660

601 cgcgttgaag tcagtccgtc ggtgggacgt cgctgtggat ttttgggtgg actcccctag 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctctgcacag tagtaactag agactagaaa aaggccgaca agtcccgctc cgctgaagat 720

661 gagacgtgtc atcattgatc tctgatcttt ttccggctgt tcagggcgag gcgacttcta 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 780

721 ggaccgcatt atcgcttctc cgggcgtggc tagcgggaag ggttgtcaac gcgtcggact 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 840

781 taccgcttac cctgcgcggg acatcgccgc gtaattcgcg ccgcccacac caccaatgcg 840

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 900

841 cgtcgcactg gcgatgtgaa cggtcgcggg atcgcgggcg aggaaagcga aagaagggaa 900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 960

901 ggaaagagcg gtgcaagcgg ccgaaagggg cagttcgaga tttagccccc gagggaaatc 960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 1020

961 ccaaggctaa atcacgaaat gccgtggagc tggggttttt tgaactaatc ccactaccaa 1020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 1080

1021 gtgcatcacc cggtagcggg actatctgcc aaaaagcggg aaactgcaac ctcaggtgca 1080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 1140

1081 agaaattatc acctgagaac aaggtttgac cttgttgtga gttgggatag agccagataa 1140

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 1200

1141 gaaaactaaa tattccctaa aacggctaaa gccggataac caatttttta ctcgactaaa 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 1260

1201 ttgtttttaa attgcgctta aaattgtttt ataattgcga atgttaaatc caccgtgaaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 1320

1261 agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat 1320

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1321 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 1380

1321 aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata 1380

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 1440

1381 ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa cggaaggaca 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 1500

1441 aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca acccacgtgc 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 1560

1501 tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 1620

1561 tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 1680

1621 ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 1740

1681 actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt ctcttaatac 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 1800

1741 gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact gttgctagcc 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 1860

1801 tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 1920

1861 agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt ggtgctacgg 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 1980

1921 acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... AseI(1991) .......... .......... .......... ..........

1981 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 2040

1981 ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg aagacgcgag 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 2100

2041 ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg cacccagagc 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 2160

2101 gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 2220

2161 ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct atccacggag 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 2280

2221 tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa 2280

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 2340

2281 ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat tagagtactg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 2400

2341 gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc ttttctagtt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 2460

2401 tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 2520

2461 tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 2580

2521 ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg gcatcaatcc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 2640

2581 ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 2700

2641 tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 2760

2701 tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 2820

2761 cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt cgcggtgcga 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 2880

2821 agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 2940

2881 gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 3000

2941 ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 3060

3001 gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa 3060

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 3120

3061 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 3180

3121 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NdeI(3233)

3181 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg 3240

3181 cgcggactac gccataaaag aggaatgcgt agacacgcca taaagtgtgg cgtataccac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg 3300

3241 gtgagagtca tgttagacga gactacggcg tatcaattcg gtcatatgtg aggcgatagc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga 3360

3301 gatgcactga cccagtaccg acgcggggct gtgggcggtt gtgggcgact gcgcgggact 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 3420

3361 gcccgaacag acgagggccg taggcgaatg tctgttcgac actggcagag gccctcgacg 3420

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... ......... PvuII(3465) .........

3421 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca 3480

3421 tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct ccgtcgacgc catttcgagt 3480

pGEX_3_pri .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg 3540

3481 agtcgcacca gcacttcgct aagtgtctac agacggacaa gtaggcgcag gtcgagcaac 3540

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt 3600

3541 tcaaagaggt cttcgcaatt acagaccgaa gactatttcg cccggtacaa ttcccgccaa 3600

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg 3660

3601 aaaaggacaa accagtgact acggaggcac attcccccta aagacaagta cccccattac 3660

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg 3720

3661 tatggctact ttgctctctc ctacgagtgc tatgcccaat gactactact tgtacgggcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttactggaac gg........ .......... .......... .......... .......... 3732

3721 aatgaccttg cc........ .......... .......... .......... .......... 3732

.......... .......... .......... .......... .......... ..........