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    Plasmid map of pGP-FF

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    View the sequence with features on line
    Fusion proteins or tags: Gal11P
    Terminal: N terminal on backbone
    Vector backbone: pGP-FF
    Type of vector: Bacterial expression
    Backbone size (bp): 3483
    Cloning site 5': XbaI
    Site destroyed during cloning: No
    Cloning site 3': BsgI
    Site destroyed during cloning: No
    5' Sequencing primer: GGG GGA TTT CTG TTC ATG GGG G
    Bacteria resistance: Ampicillin
    High or low copy: Low Copy
    Grow in standard E. coli @ 37C: No
    Please specify bacterial strain for growth and growth condition: Needs strain with lacIq Plasmid Provided In: XL1 Blue
    Principal Investigator: Keith Joung
    Comments: Bacterial cell-based two-hybrid (B2H) expression vector. Encodes zinc finger-Gal11P hybrid protein. This plasmid is a corrected version of pGP-FB. Note that the BsgI site (not pictured in the map below) cuts at base 420. pGP-FF requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.

    image of pGP-FF

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