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pGP-FF
Fusion proteins or tags: Gal11P
Terminal: N terminal on backbone
Vector backbone: pGP-FF
Type of vector: Bacterial expression
Backbone size (bp): 3483
Cloning site 5': XbaI
Site destroyed during cloning: No
Cloning site 3': BsgI
Site destroyed during cloning: No
5' Sequencing primer: GGG GGA TTT CTG TTC ATG GGG G
Bacteria resistance: Ampicillin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Needs strain with lacIq Plasmid Provided In: XL1 Blue
Principal Investigator: Keith Joung
Comments: Bacterial cell-based two-hybrid (B2H) expression vector. Encodes zinc finger-Gal11P hybrid protein. This plasmid is a corrected version of pGP-FB. Note that the BsgI site (not pictured in the map below) cuts at base 420. pGP-FF requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.