Sequence of pGP-FF

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Sequence of pGP-FF

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Sequence of pGP-FF : 3503 BP; 880 A; 878 C; 861 G; 884 T; 0 other;

EcoRI(1) .......... .......... .......... SalI(44) ..........

1 gaattccggg ctttacactt tatgcttccg gctcgtataa tgtgtcgact tgtgagcgga 60

1 cttaaggccc gaaatgtgaa atacgaaggc cgagcatatt acacagctga acactcgcct 60

.......... ......... lac_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 taacaatttc acacaggaaa cagctatgcc tcaacagcag caaatgcaac ctcccaattc 120

61 attgttaaag tgtgtccttt gtcgatacgg agttgtcgtc gtttacgttg gagggttaag 120

M13_pUC_rev_primer .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagtgcggac aacaaccctt tgcaacagca atcatcacaa aataccgtac caaacgtcct 180

121 ttcacgcctg ttgttgggaa acgttgtcgt tagtagtgtt ttatggcatg gtttgcagga 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaccaaatt aaccaaatct tttctccaga ggagcaacgc agcttattac aagaagccat 240

181 gttggtttaa ttggtttaga aaagaggtct cctcgttgcg tcgaataatg ttcttcggta 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cgaaacctgc aagaattttg aaaaaacaca attgggtagt acgatgacgg aacctgtcaa 300

241 gctttggacg ttcttaaaac ttttttgtgt taacccatca tgctactgcc ttggacagtt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... HindIII(351)

301 gcaaagtttt attaggaaat acattgtgca aaaggccctg agaaaaatcc aagctttggc 360

301 cgtttcaaaa taatccttta tgtaacacgt tttccgggac tctttttagg ttcgaaaccg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NotI(392) .......... ..........

361 ggccgctgac tacaaagacg atgatgacaa atctagagtg cagtgataac ctctactcac 420

361 ccggcgactg atgtttctgc tactactgtt tagatctcac gtcactattg gagatgagtg 420

.......... .......... .......... .......... .......... ..........

BamHI(427) .......... .......... .......... .......... ..........

421 ctgcgtggat cctctgcaca gtagtaacta gagactagaa aaaggccgac aagtcccgct 480

421 gacgcaccta ggagacgtgt catcattgat ctctgatctt tttccggctg ttcagggcga 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ccgctgaaga tcctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 540

481 ggcgacttct aggaccgcat tatcgcttct ccgggcgtgg ctagcgggaa gggttgtcaa 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 600

541 cgcgtcggac ttaccgctta ccctgcgcgg gacatcgccg cgtaattcgc gccgcccaca 600

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

601 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 660

601 ccaccaatgc gcgtcgcact ggcgatgtga acggtcgcgg gatcgcgggc gaggaaagcg 660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 720

661 aaagaaggga aggaaagagc ggtgcaagcg gccgaaaggg gcagttcgag atttagcccc 720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 780

721 cgagggaaat cccaaggcta aatcacgaaa tgccgtggag ctggggtttt ttgaactaat 780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 840

781 cccactacca agtgcatcac ccggtagcgg gactatctgc caaaaagcgg gaaactgcaa 840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 900

841 cctcaggtgc aagaaattat cacctgagaa caaggtttga ccttgttgtg agttgggata 900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 960

901 gagccagata agaaaactaa atattcccta aaacggctaa agccggataa ccaatttttt 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 1020

961 actcgactaa attgttttta aattgcgctt aaaattgttt tataattgcg aatgttaaat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 1080

1021 ccaccgtgaa aagccccttt acacgcgcct tggggataaa caaataaaaa gatttatgta 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 1140

1081 agtttataca taggcgagta ctctgttatt gggactattt acgaagttat tataactttt 1140

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 1200

1141 tccttctcat actcataagt tgtaaaggca cagcgggaat aagggaaaaa acgccgtaaa 1200

.......... .......... .... Orf frame 1 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

1201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 1260

1201 acggaaggac aaaaacgagt gggtctttgc gaccactttc attttctacg acttctagtc 1260

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 1320

1261 aacccacgtg ctcacccaat gtagcttgac ctagagttgt cgccattcta ggaactctca 1320

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 1380

1321 aaagcggggc ttcttgcaaa aggttactac tcgtgaaaat ttcaagacga tacaccgcgc 1380

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 1440

1381 cataataggg cataactgcg gcccgttctc gttgagccag cggcgtatgt gataagagtc 1440

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 1500

1441 ttactgaacc aactcatgag tggtcagtgt cttttcgtag aatgcctacc gtactgtcat 1500

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 1560

1501 tctcttaata cgtcacgacg gtattggtac tcactattgt gacgccggtt gaatgaagac 1560

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 1620

1561 tgttgctagc ctcctggctt cctcgattgg cgaaaaaacg tgttgtaccc cctagtacat 1620

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 1680

1621 tgagcggaac tagcaaccct tggcctcgac ttacttcggt atggtttgct gctcgcactg 1680

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 1740

1681 tggtgctacg gacatcgtta ccgttgttgc aacgcgtttg ataattgacc gcttgatgaa 1740

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... AseI(1762) .......... .......... ..........

1741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 1800

1741 tgagatcgaa gggccgttgt taattatctg acctacctcc gcctatttca acgtcctggt 1800

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 1860

1801 gaagacgcga gccgggaagg ccgaccgacc aaataacgac tatttagacc tcggccactc 1860

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 1920

1861 gcacccagag cgccatagta acgtcgtgac cccggtctac cattcgggag ggcatagcat 1920

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 1980

1921 caatagatgt gctgcccctc agtccgttga tacctacttg ctttatctgt ctagcgactc 1980

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 2040

1981 tatccacgga gtgactaatt cgtaaccatt gacagtctgg ttcaaatgag tatatatgaa 2040

.... Orf frame 1 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 2100

2041 atctaactaa attttgaagt aaaaattaaa ttttcctaga tccacttcta ggaaaaacta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 2160

2101 ttagagtact ggttttaggg aattgcactc aaaagcaagg tgactcgcag tctggggcat 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 2220

2161 cttttctagt ttcctagaag aactctagga aaaaaagacg cgcattagac gacgaacgtt 2220

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 2280

2221 tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc tagttctcga tggttgagaa 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 2340

2281 aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt tatgacagga agatcacatc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 2400

2341 ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg gatgtatgga gcgagacgat 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 2460

2401 taggacaatg gtcaccgacg acggtcaccg ctattcagca cagaatggcc caacctgagt 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 2520

2461 tctgctatca atggcctatt ccgcgtcgcc agcccgactt gccccccaag cacgtgtgtc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 2580

2521 gggtcgaacc tcgcttgctg gatgtggctt gactctatgg atgtcgcact cgatactctt 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 2640

2581 tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag gccattcgcc gtcccagcct 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 2700

2641 tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga ccatagaaat atcaggacag 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 2760

2701 cccaaagcgg tggagactga actcgcagct aaaaacacta cgagcagtcc ccccgcctcg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 2820

2761 gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac gaccggaaaa 2820

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 2880

2821 cgagtgtaca agaaaggacg caatagggga ctaagacacc tattggcata atggcggaaa 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 2940

2881 ctcactcgac tatggcgagc ggcgtcggct tgctggctcg cgtcgctcag tcactcgctc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac 3000

2941 cttcgccttc tcgcggacta cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg 3000

.......... .......... .......... .......... .......... ..........

NdeI(3004) .......... .......... .......... .......... ..........

3001 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca 3060

3001 gcgtatacca cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcatatgt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg 3120

3061 gaggcgatag cgatgcactg acccagtacc gacgcggggc tgtgggcggt tgtgggcgac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 3180

3121 tgcgcgggac tgcccgaaca gacgagggcc gtaggcgaat gtctgttcga cactggcaga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... PvuII(3236)

3181 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc 3240

3181 ggccctcgac gtacacagtc tccaaaagtg gcagtagtgg ctttgcgcgc tccgtcgacg 3240

........ pGEX_3_primer ........ ROP .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt 3300

3241 ccatttcgag tagtcgcacc agcacttcgc taagtgtcta cagacggaca agtaggcgca 3300

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt 3360

3301 ggtcgagcaa ctcaaagagg tcttcgcaat tacagaccga agactatttc gcccggtaca 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca 3420

3361 attcccgcca aaaaaggaca aaccagtgac tacggaggca cattccccct aaagacaagt 3420

.......... ROP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg 3480

3421 acccccatta ctatggctac tttgctctct cctacgagtg ctatgcccaa tgactactac 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aacatgcccg gttactggaa cgg....... .......... .......... .......... 3503

3481 ttgtacgggc caatgacctt gcc....... .......... .......... .......... 3503

.......... .......... .......... .......... .......... ..........