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    Plasmid map of pGT2

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    View the sequence with features on line
    Vector backbone: pGT2
    Type of vector: Bacterial expression
    Backbone size (bp): 3267
    Cloning site 5': XcmI, HindIII, StyI, BspEI, EcoRV
    Site destroyed during cloning: No
    Cloning site 3': XcmI, SacI, SpeI, NotI, EcoRI, AatII
    Site destroyed during cloning: No
    5' Sequencing primer: M13/pUC Reverse (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Modified from pGFPTA (Husimi et al, 2000)
    Comments: pGT2 is an XcmI generated T-vector bearing GFP marker optimized for direct cloning. Colonies with insert are selected by GFP inactivation.



    image of pGT2



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