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Vector backbone: pGT2
Type of vector: Bacterial expression
Backbone size (bp): 3267
Cloning site 5': XcmI, HindIII, StyI, BspEI, EcoRV
Site destroyed during cloning: No
Cloning site 3': XcmI, SacI, SpeI, NotI, EcoRI, AatII
Site destroyed during cloning: No
5' Sequencing primer: M13/pUC Reverse (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Modified from pGFPTA (Husimi et al, 2000)
Comments: pGT2 is an XcmI generated T-vector bearing GFP marker optimized for direct cloning. Colonies with insert are selected by GFP inactivation.