QkQkaLOCUS pGT2 3267 bp DNA SYN 31-Aug-2007 DEFINITION pGT2 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3267 /organism="pGT2" /mol_type="other DNA" promoter 88..117 /label="lac_promoter" misc_feature 131..153 /label="M13_pUC_rev_primer" misc_feature 152..170 /label="M13_reverse_primer" misc_feature complement(264..292) /label="GFP_R_primer" misc_feature complement(308..327) /label="GFPfusionREV_primer" CDS 162..956 /label="GFP_cyc3(variant)" /gene="GFP_cyc3(variant)" /note="ORF frame 3" /translation="MTMITPSLHACRSTLEDPRVPVEKMSKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM KRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNI LGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVL LPDNHYLSTQSALLKDPNEKRDHMVLLEFVTAAGITHGMDELYKPH*" gene 234..941 /label="GFP_cyc3(variant)" /gene="GFP_cyc3(variant)" misc_feature 887..907 /label="GFP_F_primer" promoter 1111..1139 /label="AmpR_promoter" CDS 1181..2041 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1181..2041 /label="Ampicillin" /gene="Ampicillin" rep_origin 2196..2815 /label="pBR322_origin" misc_feature 3212..3234 /label="pGEX_3_primer" ORIGIN 1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 181 gcttgcatgc ctgcaggtcg actctagagg atcccAgggt TccggtGgaa aaaatgagta 241 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 301 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 361 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 421 ctctgacgta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact 481 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cacgatatct ttcaaagatg 541 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta 601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt 661 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag 721 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc 781 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga 841 cacaatctgc ccttttgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt 901 ttgtaactgc tgctgggatt acacatggca tggatgagct CTACAAACCA CACTAGTGCT 961 GGCGGCCGCG aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa 1021 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 1081 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 1141 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 1201 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 1261 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 1321 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 1381 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga 1441 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 1501 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 1561 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 1621 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 1681 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac 1741 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 1801 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 1861 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 1921 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 1981 tatggatgaa cgaaatagac agatcgctga gataggtgcg tcactgatta agcattggta 2041 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 2101 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 2161 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2221 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2281 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2341 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2401 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 2461 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 2521 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 2581 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 2641 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 2701 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 2761 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 2821 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 2881 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 2941 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt 3001 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg 3061 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg 3121 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 3181 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 3241 ccgtcatcac cgaaacgcgc gaggcag // pGT2 "Times New RomanQkC Xulac_pro"Times New RomanQkC M13_pUC_rev_primer"Times New RomanQkCɆ M13_reverse_primer"Times New RomanQkC ORF3"Times New Roman(QkCɆ GFP_cyc3(variant)"Times New RomanQk $ GFP_R_primer"Times New RomanQkC 4GGFPfusionREV_primer"Times New RomanQk w GFP_F_primer"Times New RomanQkC Ws AmpR_promoter"Times New RomanQkC  Orf frame 2"Times New RomanQkCɆ  Ampicillin"Times New RomanQk   pBR322_origin"Times New RomanQkC  pGEX_3_primer"Times New Roman QkHindIII"Times New Roman  \I;,; QkXbaI"Times New Roman _M:+: QkNcoI(399), MscI(404)"Times New Roman , QkEcoRV"Times New Roman IZjj QkXhoI"Times New Roman  `s Qk6SacI(940), SpeI(952), NotI(963), EagI(963), EcoRI(970)"Times New Roman bSrWR-w QkAatII"Times New Roman# Vqiz{{{ QkFspI"Times New Roman tpk L  Qktest"Times New Roman 5DRxR<x Qktest"Times New Roman6 yQri