Sequence of pGT2

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Sequence of pGT2

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Sequence of pGT2 : 3267 BP; 858 A; 782 C; 797 G; 830 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 60

1 gaccgtgctg tccaaagggc tgacctttcg cccgtcactc gcgttgcgtt aattacactc 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 120

61 aatcgagtga gtaatccgtg gggtccgaaa tgtgaaatac gaaggccgag catacaacac 120

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... ......... HindIII(179)

121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 180

121 accttaacac tcgcctattg ttaaagtgtg tcctttgtcg atactggtac taatgcggtt 180

...... M13_pUC_rev_primer ...... ...... M13_reverse_primer ORF3

.......... .......... XbaI(203) .......... .......... ..........

181 gcttgcatgc ctgcaggtcg actctagagg atcccagggt tccggtggaa aaaatgagta 240

181 cgaacgtacg gacgtccagc tgagatctcc tagggtccca aggccacctt ttttactcat 240

.......... .......... ORF3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 300

241 ttcctcttct tgaaaagtga cctcaacagg gttaagaaca acttaatcta ccactacaat 300

.......... ... ORF3 GFP_cyc3(variant) GFP_R_primer ... ..........

.......... .......... .......... .......... .......... ..........

301 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 360

301 tacccgtgtt taaaagacag tcacctctcc cacttccact acgttgtatg ccttttgaat 360

GFPfusionREV_primer ORF3 GFP_cyc3(variant) ........ ..........

.......... .......... .......... ..... NcoI(399),MscI(404) .....

361 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 420

361 gggaatttaa ataaacgtga tgaccttttg atggacaagg taccggttgt gaacagtgat 420

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctctgacgta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact 480

421 gagactgcat accacaagtt acgaaaaggg caataggcct agtatacttt gccgtactga 480

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... EcoRV(526) ..........

481 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cacgatatct ttcaaagatg 540

481 aaaagttctc acggtacggg cttccaatac atgtccttgc gtgctataga aagtttctac 540

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

541 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta 600

541 tgcccttgat gttctgcgca cgacttcagt tcaaacttcc actatgggaa caattagcat 600

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... XhoI(654)

601 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt 660

601 agctcaattt tccataacta aaatttcttc tacctttgta agagcctgtg tttgagctca 660

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag 720

661 tgttgatatt gagtgtgtta catatgtagt gccgtctgtt tgttttctta ccttagtttc 720

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc 780

721 gattgaagtt ttaagcggtg ttgtaacttc tacctaggca agttgatcgt ctggtaatag 780

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga 840

781 ttgttttatg aggttaaccg ctaccgggac aggaaaatgg tctgttggta atggacagct 840

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cacaatctgc ccttttgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt 900

841 gtgttagacg ggaaaacttt ctagggttgc ttttcgcact ggtgtaccag gaagaactca 900

.......... .... ORF3 GFP_cyc3(variant) .... ......... GFP_F_primer

.......... .......... .......... SacI(940) .......... ..........

901 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaacca cactagtgct 960

901 aacattgacg acgaccctaa tgtgtaccgt acctactcga gatgtttggt gtgatcacga 960

.......... .... ORF3 GFP_cyc3(variant) .... .......... ..........

NotI(963),EagI(963) EcoRI(970) .......... .......... ..........

961 ggcggccgcg aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa 1020

961 ccgccggcgc ttaagaactt ctgctttccc ggagcactat gcggataaaa atatccaatt 1020

.......... .......... .......... .......... .......... ..........

.......... ......... AatII(1049) ......... .......... ..........

1021 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg 1080

1021 acagtactat tattaccaaa gaatctgcag tccaccgtga aaagcccctt tacacgcgcc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 1140

1081 ttggggataa acaaataaaa agatttatgt aagtttatac ataggcgagt actctgttat 1140

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

1141 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg 1200

1141 tgggactatt tacgaagtta ttataacttt ttccttctca tactcataag ttgtaaaggc 1200

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1201 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 1260

1201 acagcgggaa taagggaaaa aacgccgtaa aacggaagga caaaaacgag tgggtctttg 1260

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 1320

1261 cgaccacttt cattttctac gacttctagt caacccacgt gctcacccaa tgtagcttga 1320

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 1380

1321 cctagagttg tcgccattct aggaactctc aaaagcgggg cttcttgcaa aaggttacta 1380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga 1440

1381 ctcgtgaaaa tttcaagacg atacaccgcg ccataatagg gcacaactgc ggcccgttct 1440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 1500

1441 cgttgagcca gcggcgtatg tgataagagt cttactgaac caactcatga gtggtcagtg 1500

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 1560

1501 tcttttcgta gaatgcctac cgtactgtca ttctcttaat acgtcacgac ggtattggta 1560

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 1620

1561 ctcactattg tgacgccggt tgaatgaaga ctgttgctag cctcctggct tcctcgattg 1620

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 1680

1621 gcgaaaaaac gtgttgtacc ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga 1680

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac 1740

1681 cttacttcgg tatggtttgc tgctcgcact gtggtgctac ggacgtcgtt accgttgttg 1740

.......... .... Orf frame 2 Ampicillin .... .......... ..........

FspI(1745) .......... .......... .......... .......... ..........

1741 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 1800

1741 caacgcgttt gataattgac cgcttgatga atgagatcga agggccgttg ttaattatct 1800

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 1860

1801 gacctacctc cgcctatttc aacgtcctgg tgaagacgcg agccgggaag gccgaccgac 1860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 1920

1861 caaataacga ctatttagac ctcggccact cgcacccaga gcgccatagt aacgtcgtga 1920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 1980

1921 ccccggtcta ccattcggga gggcatagca tcaatagatg tgctgcccct cagtccgttg 1980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tatggatgaa cgaaatagac agatcgctga gataggtgcg tcactgatta agcattggta 2040

1981 atacctactt gctttatctg tctagcgact ctatccacgc agtgactaat tcgtaaccat 2040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt 2100

2041 tgacagtctg gttcaaatga gtatatatga aatctaacta aattttgaag taaaaattaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 2160

2101 attttcctag atccacttct aggaaaaact attagagtac tggttttagg gaattgcact 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 2220

2161 caaaagcaag gtgactcgca gtctggggca tcttttctag tttcctagaa gaactctagg 2220

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2221 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 2280

2221 aaaaaaagac gcgcattaga cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 2340

2281 aacaaacggc ctagttctcg atggttgaga aaaaggcttc cattgaccga agtcgtctcg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 2400

2341 cgtctatggt ttatgacagg aagatcacat cggcatcaat ccggtggtga agttcttgag 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 2460

2401 acatcgtggc ggatgtatgg agcgagacga ttaggacaat ggtcaccgac gacggtcacc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 2520

2461 gctattcagc acagaatggc ccaacctgag ttctgctatc aatggcctat tccgcgtcgc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 2580

2521 cagcccgact tgccccccaa gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 2640

2581 tgactctatg gatgtcgcac tcgatactct ttcgcggtgc gaagggcttc cctctttccg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 2700

2641 cctgtccata ggccattcgc cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 2760

2701 ccctttgcgg accatagaaa tatcaggaca gcccaaagcg gtggagactg aactcgcagc 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 2820

2761 taaaaacact acgagcagtc cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 2880

2821 aaatgccaag gaccggaaaa cgaccggaaa acgagtgtac aagaaaggac gcaatagggg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 2940

2881 actaagacac ctattggcat aatggcggaa actcactcga ctatggcgag cggcgtcggc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt 3000

2941 ttgctggctc gcgtcgctca gtcactcgct ccttcgcctt ctcgcggact acgccataaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg 3060

3001 agaggaatgc gtagacacgc cataaagtgt ggcgtatacc acgtgagagt catgttagac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg 3120

3061 gagactacgg cgtatcaatt cggtcatatg tgaggcgata gcgatgcact gacccagtac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 3180

3121 cgacgcgggg ctgtgggcgg ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 3240

3181 cgtaggcgaa tgtctgttcg acactggcag aggccctcga cgtacacagt ctccaaaagt 3240

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

3241 ccgtcatcac cgaaacgcgc gaggcag... .......... .......... .......... 3267

3241 ggcagtagtg gctttgcgcg ctccgtc... .......... .......... .......... 3267

.......... .......... .......... .......... .......... ..........