e1e1LOCUS pGeneClip Basic 3402 bp DNA SYN 08-Nov-2007 DEFINITION pGeneClip Basic ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3402 /organism="pGeneClip Basic" /mol_type="other DNA" CDS complement(3234..476) /label="ORF frame 3" /translation="MRPPSLLLKLQTARSWASAPTQPHTPHSLHTRSLAPRTPEPFPA LTHSWDSHFRSAVLNPLPGHGNSFVVKQQVAKQQPLRCLIYIEVALSVMSPTVPCPVP VCLSATQPTNLLPEPPRGSDVCLSLFPSRPCVCSLPKKLVLRDPNSSTLEISGPIRPI VSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA*" misc_feature complement(529..546) /label="Sp6_primer" misc_feature complement(560..578) /label="M13_reverse_primer" misc_feature complement(577..599) /label="M13_pUC_rev_primer" promoter complement(613..642) /label="lac_promoter" rep_origin complement(951..1569) /label="pBR322_origin" CDS complement(1724..2584) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(1724..2584) /label="Ampicillin" /gene="Ampicillin" promoter complement(2626..2654) /label="AmpR_promoter" rep_origin complement(2899..3205) /label="f1_origin" misc_feature complement(3234..3382) /label="lacZ_a" misc_feature 3348..3370 /label="M13_pUC_fwd_primer" misc_feature 3363..3379 /label="M13_forward20_primer" promoter 3386..2 /label="T7_promoter" ORIGIN 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG GACCAGCTTC 61 TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG AGAGGCAGAC GTCACTTCCC 121 CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT TGAGTGGCAG AAAGGCAGAC GGGGACTGGG 181 CAAGGCACTG TCGGTGACAT CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA 241 GGCTGCTGCT TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG AAAGGGCTCG 361 GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG GCGTATGAGG CTGTGTCGGG 421 GCAGAGGCCC AAGATCTCGC AGTCTGGAGT TTCAAAAGTA GACTGGGCGG CCGCATCGAT 481 GTTAACCTCG AGGAGCTCCC AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC 541 CTAAATAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 661 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 721 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 781 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 841 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 901 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 961 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 1021 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 1081 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 1141 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 1201 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 1261 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 1321 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 1381 TGGCCTAACT ACGGCTACAC TAGAAAACAG TATTTGGTAT CTGCGCTCTG CAGAAGCCAG 1441 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 1501 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 1561 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 1621 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 1681 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 1741 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 1801 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 1861 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 1921 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 1981 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 2041 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 2101 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 2161 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 2221 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 2281 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 2341 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 2401 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 2461 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 2521 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 2581 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 2641 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 2701 GAAAAGTGCC ACCTGATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 2761 ATCAGGAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA 2821 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA 2881 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG 2941 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT 3001 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG 3061 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA 3121 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA 3181 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCATTCGC CATTCAGGCT 3241 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 3301 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 3361 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TA //J pGeneClip Basic "Times New Romane1CJ ORF 3"Times New Romane1CJ : T7_pro"Times New Romane1CJ "Sp6_pri"Times New Romane1CɆJ 0BM13_reverse_pri"Times New Romane1J AWM13_pUC_rev_pri"Times New Romane1CJ elac_pro"Times New Romane1CJ ! pBR322_origin"Times New Romane1CɆJ  ORF 2"Times New Romane1J   Ampicillin"Times New Roman(e1CJ B ^ AmpR_pro"Times New Romane1CJ S  f1_origin"Times New Romane1CɆJ 6 lacZ_a"Times New Romane1J  * M13_pUC_fwd_pri"Times New Romane1CJ # 3 M13_forward20_pri"Times New Roman) e1CApaI(14),EcoRV(18),XbaI(22),SalI(28),EcoRI(34),BamHI(40),AatII(112)"Times New Roman J e[Qz Q e1FBglII(432),NotI(468),EagI(468),ClaI(476),HpaI(483),XhoI(487),SacI(497)"Times New RomanJ LSZZ0 e1PstI"Times New RomanJ "$$?3 e1test"Times New Roman@ J e{5\ e1test"Times New RomanIJ sO22x