Sequence of pGeneClip

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Sequence of pGeneClip_Basic

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Sequence of pGeneClip Basic : 3402 BP; 864 A; 825 C; 911 G; 802 T; 0 other;

....... ApaI(14),EcoRV(18),XbaI(22) SalI(28) EcoRI(34) .......

1 gggcgaattg ggcccgatat ctctagagtc gacgaattcg gatccctaag gaccagcttc 60

1 cccgcttaac ccgggctata gagatctcag ctgcttaagc ctagggattc ctggtcgaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... AatII(112)

61 tttgggagag aacagacgca ggggcgggag ggaaaaaggg agaggcagac gtcacttccc 120

61 aaaccctctc ttgtctgcgt ccccgccctc cctttttccc tctccgtctg cagtgaaggg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttggcggct ctggcagcag attggtcggt tgagtggcag aaaggcagac ggggactggg 180

121 gaaccgccga gaccgtcgtc taaccagcca actcaccgtc tttccgtctg cccctgaccc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaggcactg tcggtgacat cacggacagg gcgacttcta tgtagatgag gcagcgcaga 240

181 gttccgtgac agccactgta gtgcctgtcc cgctgaagat acatctactc cgtcgcgtct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggctgctgct tcgccacttg ctgcttcacc acgaaggagt tcccgtgccc tgggagcggg 300

241 ccgacgacga agcggtgaac gacgaagtgg tgcttcctca agggcacggg accctcgccc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttcaggaccg ctgatcggaa gtgagaatcc cagctgtgtg tcagggctgg aaagggctcg 360

301 aagtcctggc gactagcctt cactcttagg gtcgacacac agtcccgacc tttcccgagc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggagtgcgcg gggcaagtga ccgtgtgtgt aaagagtgag gcgtatgagg ctgtgtcggg 420

361 cctcacgcgc cccgttcact ggcacacaca tttctcactc cgcatactcc gacacagccc 420

.......... .......... .......... .......... .......... ..........

.......... BglII(432) .......... ..... NotI(468),EagI(468) .....

421 gcagaggccc aagatctcgc agtctggagt ttcaaaagta gactgggcgg ccgcatcgat 480

421 cgtctccggg ttctagagcg tcagacctca aagttttcat ctgacccgcc ggcgtagcta 480

.......... .......... .......... .......... .......... ..........

HpaI(483),XhoI(487) SacI(497) .......... .......... ..........

481 gttaacctcg aggagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 540

481 caattggagc tcctcgaggg ttgcgcaacc tacgtatcga actcataaga tatcacagtg 540

.......... .......... .......... .......... .......... Sp6_pri

.......... .......... .......... .......... .......... ..........

541 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 600

541 gatttatcga accgcattag taccagtatc gacaaaggac acactttaac aataggcgag 600

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 660

601 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 660

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 720

661 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 780

721 agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 840

781 gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc agcaagccga cgccgctcgc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 900

841 catagtcgag tgagtttccg ccattatgcc aataggtgtc ttagtcccct attgcgtcct 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 960

901 ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg catttttccg gcgcaacgac 960

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 1020

961 cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt ttttagctgc gagttcagtc 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 1080

1021 tccaccgctt tgggctgtcc tgatatttct atggtccgca aagggggacc ttcgagggag 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 1140

1081 cacgcgagag gacaaggctg ggacggcgaa tggcctatgg acaggcggaa agagggaagc 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 1200

1141 ccttcgcacc gcgaaagagt atcgagtgcg acatccatag agtcaagcca catccagcaa 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 1260

1201 gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg ggctggcgac gcggaatagg 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 1320

1261 ccattgatag cagaactcag gttgggccat tctgtgctga atagcggtga ccgtcgtcgg 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 1380

1321 tgaccattgt cctaatcgtc tcgctccata catccgccac gatgtctcaa gaacttcacc 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... PstI(1432)

1381 tggcctaact acggctacac tagaaaacag tatttggtat ctgcgctctg cagaagccag 1440

1381 accggattga tgccgatgtg atcttttgtc ataaaccata gacgcgagac gtcttcggtc 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1500

1441 aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1560

1501 caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1620

1561 gaaactagaa aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa 1620

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1680

1621 accagtactc taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1740

1681 aatttagtta gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt 1740

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

1741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1800

1741 cactccgtgg atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1860

1801 agcacatcta ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1920

1861 gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1980

1921 ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2040

1981 cccttcgatc tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgat 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2100

2041 gtccgtagca ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2160

2101 ctagttccgc tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2220

2161 gaggctagca acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2280

2221 acgtattaag agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga 2280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2340

2281 gttggttcag taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt 2340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2400

2341 atgccctatt atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa 2400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2460

2401 gaagccccgc ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt 2460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2520

2461 gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt 2520

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2580

2521 tttgtccttc cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg 2580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 2640

2581 agtatgagaa ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc 2640

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

2641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 2700

2641 ctatgtataa acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg 2700

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 2760

2701 cttttcacgg tggactacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 2820

2761 tagtccttta acattcgcaa ttataaaaca attttaagcg caatttaaaa acaatttagt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 2880

2821 cgagtaaaaa attggttatc cggctttagc cgttttaggg aatatttagt tttcttatct 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 2940

2881 ggctctatcc caactcacaa caaggtcaaa ccttgttctc aggtgataat ttcttgcacc 2940

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2941 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 3000

2941 tgaggttgca gtttcccgct ttttggcaga tagtcccgct accgggtgat gcacttggta 3000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 3060

3001 gtgggattag ttcaaaaaac cccagctcca cggcatttcg tgatttagcc ttgggatttc 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 3120

3061 cctcgggggc taaatctcga actgcccctt tcggccgctt gcaccgctct ttccttccct 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 3180

3121 tctttcgctt tcctcgcccg cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt 3180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct 3240

3181 ggtggtgtgg gcggcgcgaa ttacgcggcg atgtcccgcg caggtaagcg gtaagtccga 3240

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 3300

3241 cgcgttgaca acccttcccg ctagccacgc ccggagaagc gataatgcgg tcgaccgctt 3300

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 3360

3301 tccccctaca cgacgttccg ctaattcaac ccattgcggt cccaaaaggg tcagtgctgc 3360

.......... .......... lacZ_a .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

3361 ttgtaaaacg acggccagtg aattgtaata cgactcacta ta........ .......... 3402

3361 aacattttgc tgccggtcac ttaacattat gctgagtgat at........ .......... 3402

M13_pUC_fwd_pri lacZ_a M13_forward20_pri .......... ..........