}I}I(LOCUS pGeneClip Hygromycin 4989 bp DNA SYN 14-Sep-2007 DEFINITION pGeneClip Hygromycin ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4989 /organism="pGeneClip Hygromycin" /mol_type="other DNA" CDS complement(4821..476) /label="ORF frame 3" /translation="MRPPSLLLKLQTARSWASAPTQPHTPHSLHTRSLAPRTPEPFPA LTHSWDSHFRSAVLNPLPGHGNSFVVKQQVAKQQPLRCLIYIEVALSVMSPTVPCPVP VCLSATQPTNLLPEPPRGSDVCLSLFPSRPCVCSLPKKLVLRDPNSSTLEISGPIRPI VSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA*" misc_feature complement(529..546) /label="Sp6_primer" misc_feature complement(560..578) /label="M13_reverse_primer" misc_feature complement(577..599) /label="M13_pUC_rev_primer" promoter complement(613..642) /label="lac_promoter" misc_feature complement(876..896) /label="pBABE_3_primer" misc_feature complement(882..1097) /label="SV40_enhancer" promoter 894..1162 /label="SV40_promoter" rep_origin 1061..1138 /label="SV40_origin" promoter 1014..1216 /label="SV40_promoter" misc_feature 1123..1142 /label="SV40pro_F_primer" CDS complement(1230..2009) /label="ORF frame 3" /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES TRFFALRELHQVGHAVELFDQKLLDRRRGEFRLFHGGPGDR*" CDS 1251..2276 /label="hygroB" /gene="hygroB" /note="ORF frame 3" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE*" gene 1266..2273 /label="hygroB" /gene="hygroB" terminator 2311..2359 /label="synth_PA_terminator" rep_origin complement(2537..3156) /label="pBR322_origin" CDS complement(3311..4171) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3311..4171) /label="Ampicillin" /gene="Ampicillin" promoter complement(4213..4241) /label="AmpR_promoter" rep_origin complement(4486..4792) /label="f1_origin" misc_feature complement(4821..4969) /label="lacZ_a" misc_feature 4935..4957 /label="M13_pUC_fwd_primer" misc_feature 4950..4966 /label="M13_forward20_primer" promoter 4973..2 /label="T7_promoter" ORIGIN 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG GACCAGCTTC 61 TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG AGAGGCAGAC GTCACTTCCC 121 CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT TGAGTGGCAG AAAGGCAGAC GGGGACTGGG 181 CAAGGCACTG TCGGTGACAT CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA 241 GGCTGCTGCT TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG AAAGGGCTCG 361 GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG GCGTATGAGG CTGTGTCGGG 421 GCAGAGGCCC AAGATCTCGC AGTCTGGAGT TTCAAAAGTA GACTGGGCGG CCGCATCGAT 481 GTTAACCTCG AGGAGCTCCC AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC 541 CTAAATAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 661 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 721 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 781 CGCTCTTCCG CTGATCTGCG CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG 841 GTTAGGTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 901 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 961 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 1021 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 1081 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 1141 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 1201 CTTTTGCAAA AAGCTTGATT CTTCTGACGC TAGCGATCGC CCGGGCCACC ATGAAAAAGC 1261 CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTGTCCG 1321 ACCTGATGCA GCTCTCGGAG GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC 1381 GTGGATATGT CCTGCGGGTA AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT 1441 ATCGGCACTT TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA 1501 GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC 1561 CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG 1621 CGGCCGATCT TAGCCAGACG AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT 1681 ACACTACATG GCGTGATTTC ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA 1741 CTGTGATGGA CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT 1801 GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG 1861 TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG 1921 ATTCCCAATA CGAGGTCGCC AACATCTTCT TCTGGAGGCC GTGGTTGGCT TGTATGGAGC 1981 AGCAGACGCG CTACTTCGAG CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG 2041 CGTATATGCT CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG 2101 ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA GCCGGGACTG 2161 TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG GACCGATGGC TGTGTAGAAG 2221 TACTCGCCGA TAGTGGAAAC CGACGCCCCA GCACTCGTCC GAGGGCAAAG GAATAGCTCG 2281 AGTTTAAACT CTAGAACCGG TCATGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG 2341 TGTGTTGGTT TTTTGTGTGT TCGAACTAGA AGCTTGCTTC CTCGCTCACT GACTCGCTGC 2401 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 2461 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 2521 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 2581 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 2641 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 2701 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 2761 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 2821 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 2881 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACGGGAT TAGCAGAGCG AGGTATGTAG 2941 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 3001 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 3061 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 3121 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 3181 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 3241 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 3301 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC 3361 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC 3421 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT 3481 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG 3541 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 3601 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA 3661 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT 3721 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG 3781 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA 3841 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 3901 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT 3961 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 4021 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 4081 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 4141 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 4201 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 4261 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGATGCGGTG TGAAATACCG 4321 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT AAGCGTTAAT ATTTTGTTAA 4381 AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA 4441 AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA 4501 ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC 4561 AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC 4621 GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC 4681 CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG 4741 CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC 4801 AGGGCGCGTC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 4861 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT 4921 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TGTAATACGA 4981 CTCACTATA //}pGeneClip Hygromycin "Times New Roman"}IC}ORF 3"Times New Roman}I}mT7_pro"Times New Roman}IC}"Sp6_pri"Times New Roman}ICɆ}0BM13_reverse_pri"Times New Roman}I}AWM13_pUC_rev_pri"Times New Roman)}IC}elac_pro"Times New Roman}IC}l pBABE_3_pri"Times New Roman}ICɆ}rISV40_enh"Times New Roman<}I}~SV40_pro"Times New Roman(}IC}SV40_pro"Times New Roman}IC}%r SV40_origin"Times New RomanQ}ICɆ}cv SV40pro_F_pri"Times New Roman=}I}ORF 3"Times New Roman}IC}ORF 3"Times New Roman(}IC}hygroB"Times New Roman<}ICɆ} 7  synth_PA_term"Times New Roman}I} T  pBR322_origin"Times New Roman}IC} KORF 2"Times New Roman*}IC} K Ampicillin"Times New Roman}ICɆ}uAmpR_pro"Times New Roman}I} f1_origin"Times New Roman}IC}ilacZ_a"Times New Roman)}IC}G]M13_pUC_fwd_pri"Times New Roman}ICɆ}VfM13_forward20_pri"Times New Roman }I%ApaI(14),EcoRV(18),SalI(28),BamHI(40)"Times New Roman }}sjt.j }I2BglII(432),NotI(468),ClaI(476),HpaI(483),SacI(497)"Times New Roman}16;;Ax }IKpnI"Times New RomanQ} }I StuI(1194),NheI(1229),SmaI(1242)"Times New Roman}RRQ;g }IPstI(1588),NdeI(1701)"Times New Roman4} }ISacII"Times New Roman};!A)F1F1@ }IAgeI(2296),BstBI(2361)"Times New Roman}A2 }Itest"Times New Romano}}RU }Itest"Times New Romana}We|| }Itest"Times New Roman}~&M$MQ