Sequence of pGeneClip

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Sequence of pGeneClip_Hygromycin

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Sequence of pGeneClip Hygromycin : 4989 BP; 1206 A; 1257 C; 1362 G; 1164 T; 0 other;

...... ApaI(14),EcoRV(18) SalI(28) BamHI(40) ...... ..........

1 gggcgaattg ggcccgatat ctctagagtc gacgaattcg gatccctaag gaccagcttc 60

1 cccgcttaac ccgggctata gagatctcag ctgcttaagc ctagggattc ctggtcgaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tttgggagag aacagacgca ggggcgggag ggaaaaaggg agaggcagac gtcacttccc 120

61 aaaccctctc ttgtctgcgt ccccgccctc cctttttccc tctccgtctg cagtgaaggg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttggcggct ctggcagcag attggtcggt tgagtggcag aaaggcagac ggggactggg 180

121 gaaccgccga gaccgtcgtc taaccagcca actcaccgtc tttccgtctg cccctgaccc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaggcactg tcggtgacat cacggacagg gcgacttcta tgtagatgag gcagcgcaga 240

181 gttccgtgac agccactgta gtgcctgtcc cgctgaagat acatctactc cgtcgcgtct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggctgctgct tcgccacttg ctgcttcacc acgaaggagt tcccgtgccc tgggagcggg 300

241 ccgacgacga agcggtgaac gacgaagtgg tgcttcctca agggcacggg accctcgccc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttcaggaccg ctgatcggaa gtgagaatcc cagctgtgtg tcagggctgg aaagggctcg 360

301 aagtcctggc gactagcctt cactcttagg gtcgacacac agtcccgacc tttcccgagc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggagtgcgcg gggcaagtga ccgtgtgtgt aaagagtgag gcgtatgagg ctgtgtcggg 420

361 cctcacgcgc cccgttcact ggcacacaca tttctcactc cgcatactcc gacacagccc 420

.......... .......... .......... .......... .......... ..........

.......... BglII(432) .......... .......... NotI(468) ..........

421 gcagaggccc aagatctcgc agtctggagt ttcaaaagta gactgggcgg ccgcatcgat 480

421 cgtctccggg ttctagagcg tcagacctca aagttttcat ctgacccgcc ggcgtagcta 480

.......... .......... .......... .......... .......... ..........

HpaI(483) SacI(497) .......... .......... .......... ..........

481 gttaacctcg aggagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 540

481 caattggagc tcctcgaggg ttgcgcaacc tacgtatcga actcataaga tatcacagtg 540

.......... .......... .......... .......... .......... Sp6_pri

.......... .......... .......... .......... .......... ..........

541 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 600

541 gatttatcga accgcattag taccagtatc gacaaaggac acactttaac aataggcgag 600

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 660

601 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 660

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 720

661 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 780

721 agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg 840

781 gcgagaaggc gactagacgc gtcgtggtac cggactttat tggagacttt ctccttgaac 840

.......... .......... .......... .......... .......... ..........

KpnI(849) .......... .......... .......... .......... ..........

841 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 900

841 caatccatgg aagactccgc ctttcttggt cgacacctta cacacagtca atcccacacc 900

.......... .......... .......... ......... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 960

901 tttcaggggt ccgaggggtc gtccgtcttc atacgtttcg tacgtagagt taatcagtcg 960

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 1020

961 ttggtccaca cctttcaggg gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga 1020

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 1080

1021 gttaatcagt cgttggtatc agggcgggga ttgaggcggg tagggcgggg attgaggcgg 1080

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

1081 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 1140

1081 gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa aaataaatac gtctccggct 1140

SV40_enh SV40_pro SV40_pro SV40_origin ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

1141 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 1200

1141 ccggcggagc cggagactcg ataaggtctt catcactcct ccgaaaaaac ctccggatcc 1200

.......... SV40_pro SV40_pro .......... .......... ..........

.......... .......... NheI(1229) .......... SmaI(1242) ..........

1201 cttttgcaaa aagcttgatt cttctgacgc tagcgatcgc ccgggccacc atgaaaaagc 1260

1201 gaaaacgttt ttcgaactaa gaagactgcg atcgctagcg ggcccggtgg tactttttcg 1260

SV40_pro .......... .......... .......... ORF 3 ORF 3

.......... .......... .......... .......... .......... ..........

1261 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtgtccg 1320

1261 gacttgagtg gcgctgcaga cagctcttca aagactagct tttcaagctg tcgcacaggc 1320

.......... ......... ORF 3 ORF 3 hygroB ......... ..........

.......... .......... .......... .......... .......... ..........

1321 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc 1380

1321 tggactacgt cgagagcctc ccgcttctta gagcacgaaa gtcgaagcta catcctcccg 1380

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt 1440

1381 cacctataca ggacgcccat ttatcgacgc ggctaccaaa gatgtttcta gcaatacaaa 1440

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca 1500

1441 tagccgtgaa acgtagccgg cgcgagggct aaggccttca cgaactgtaa ccccttaagt 1500

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc 1560

1501 cgctctcgga ctggataacg tagagggcgg cacgtgtccc acagtgcaac gttctggacg 1560

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg 1620

1561 gactttggct tgacgggcga caagacgtcg gccagcgcct ccggtaccta cgctagcgac 1620

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat 1680

1621 gccggctaga atcggtctgc tcgcccaagc cgggtaagcc tggcgttcct tagccagtta 1680

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... NdeI(1701) .......... .......... ..........

1681 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa 1740

1681 tgtgatgtac cgcactaaag tatacgcgct aacgactagg ggtacacata gtgaccgttt 1740

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt 1800

1741 gacactacct gctgtggcag tcacgcaggc agcgcgtccg agagctactc gactacgaaa 1800

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg 1860

1801 cccggctcct gacggggctt caggccgtgg agcacgtgcg cctaaagccg aggttgttac 1860

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg 1920

1861 aggactgcct gttaccggcg tattgtcgcc agtaactgac ctcgctccgc tacaagcccc 1920

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc 1980

1921 taagggttat gctccagcgg ttgtagaaga agacctccgg caccaaccga acatacctcg 1980

.......... ...... ORF 3 ORF 3 hygroB ...... .......... ..........

.......... .......... .......... .......... ......... SacII(2031)

1981 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg 2040

1981 tcgtctgcgc gatgaagctc gcctccgtag gcctcgaacg tcctagcggc gccgaggccc 2040

......... ORF 3 ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg 2100

2041 gcatatacga ggcgtaacca gaactggttg agatagtctc gaaccaactg ccgttaaagc 2100

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg 2160

2101 tactacgtcg aacccgcgtc ccagctacgc tgcgttagca ggctaggcct cggccctgac 2160

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag 2220

2161 agcccgcatg tgtttagcgg gcgtcttcgc gccggcagac ctggctaccg acacatcttc 2220

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagctcg 2280

2221 atgagcggct atcacctttg gctgcggggt cgtgagcagg ctcccgtttc cttatcgagc 2280

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 agtttaaact ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg 2340

2281 tcaaatttga gatcttggcc agtaccggcg ttattttata gaaataaaag taatgtagac 2340

.......... .......... .......... ........ synth_PA_term ........

.......... ......... BstBI(2361) ......... .......... ..........

2341 tgtgttggtt ttttgtgtgt tcgaactaga agcttgcttc ctcgctcact gactcgctgc 2400

2341 acacaaccaa aaaacacaca agcttgatct tcgaacgaag gagcgagtga ctgagcgacg 2400

synth_PA_term ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 2460

2401 cgagccagca agccgacgcc gctcgccata gtcgagtgag tttccgccat tatgccaata 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 2520

2461 ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc gttttccggt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 2580

2521 ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg 2580

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 2640

2581 tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat atttctatgg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 2700

2641 tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac ggcgaatggc 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 2760

2701 ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg agtgcgacat 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2820

2761 ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg cttggggggc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2880

2821 aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg ggccattctg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acgacttatc gccactggca gcagccactg gtaacgggat tagcagagcg aggtatgtag 2940

2881 tgctgaatag cggtgaccgt cgtcggtgac cattgcccta atcgtctcgc tccatacatc 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 3000

2941 cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct tcttgtcata 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 3060

3001 aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca tcgagaacta 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 3120

3061 ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 3180

3121 cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga ctgcgagtca 3180

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 3240

3181 ccttgctttt gagtgcaatt ccctaaaacc agtactctaa tagtttttcc tagaagtgga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 3300

3241 tctaggaaaa tttaattttt acttcaaaat ttagttagat ttcatatata ctcatttgaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 3360

3301 ccagactgtc aatggttacg aattagtcac tccgtggata gagtcgctag acagataaag 3360

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3361 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 3420

3361 caagtaggta tcaacggact gaggggcagc acatctattg atgctatgcc ctcccgaatg 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 3480

3421 gtagaccggg gtcacgacgt tactatggcg ctctgggtgc gagtggccga ggtctaaata 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 3540

3481 gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt tgaaataggc 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 3600

3541 ggaggtaggt cagataatta acaacggccc ttcgatctca ttcatcaagc ggtcaattat 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 3660

3601 caaacgcgtt gcaacaacgg taacgatgtc cgtagcacca cagtgcgagc agcaaaccat 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 3720

3661 accgaagtaa gtcgaggcca agggttgcta gttccgctca atgtactagg gggtacaaca 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 3780

3721 cgttttttcg ccaatcgagg aagccaggag gctagcaaca gtcttcattc aaccggcgtc 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 3840

3781 acaatagtga gtaccaatac cgtcgtgacg tattaagaga atgacagtac ggtaggcatt 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 3900

3841 ctacgaaaag acactgacca ctcatgagtt ggttcagtaa gactcttatc acatacgccg 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 3960

3901 ctggctcaac gagaacgggc cgcagttatg ccctattatg gcgcggtgta tcgtcttgaa 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 4020

3961 attttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc tagaatggcg 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 4080

4021 acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt cgtagaaaat 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 4140

4081 gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt tttttccctt 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 4200

4141 attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata ataacttcgt 4200

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 4260

4201 aaatagtccc aataacagag tactcgccta tgtataaact tacataaatc tttttatttg 4260

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgatgcggtg tgaaataccg 4320

4261 tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actacgccac actttatggc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa 4380

4321 gtgtctacgc attcctcttt tatggcgtag tcctttaaca ttcgcaatta taaaacaatt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 4440

4381 ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg ctttagccgt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 4500

4441 tttagggaat atttagtttt cttatctggc tctatcccaa ctcacaacaa ggtcaaacct 4500

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

4501 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 4560

4501 tgttctcagg tgataatttc ttgcacctga ggttgcagtt tcccgctttt tggcagatag 4560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 4620

4561 tcccgctacc gggtgatgca cttggtagtg ggattagttc aaaaaacccc agctccacgg 4620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 4680

4621 catttcgtga tttagccttg ggatttccct cgggggctaa atctcgaact gcccctttcg 4680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 4740

4681 gccgcttgca ccgctctttc cttcccttct ttcgctttcc tcgcccgcga tcccgcgacc 4740

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 4800

4741 gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta cgcggcgatg 4800

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 4860

4801 tcccgcgcag gtaagcggta agtccgacgc gttgacaacc cttcccgcta gccacgcccg 4860

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt 4920

4861 gagaagcgat aatgcggtcg accgctttcc ccctacacga cgttccgcta attcaaccca 4920

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga 4980

4921 ttgcggtccc aaaagggtca gtgctgcaac attttgctgc cggtcactta acattatgct 4980

.......... lacZ_a M13_pUC_fwd_pri M13_forward20_pri ..........

.......... .......... .......... .......... .......... ..........

4981 ctcactata. .......... .......... .......... .......... .......... 4989

4981 gagtgatat. .......... .......... .......... .......... .......... 4989

.......... .......... .......... .......... .......... ..........