j%LOCUS pGeneClip Neomycin 4758 bp DNA SYN 13-Sep-2007 DEFINITION pGeneClip Neomycin ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4758 /organism="pGeneClip Neomycin" /mol_type="other DNA" CDS complement(4590..476) /label="ORF frame 3" /translation="MRPPSLLLKLQTARSWASAPTQPHTPHSLHTRSLAPRTPEPFPA LTHSWDSHFRSAVLNPLPGHGNSFVVKQQVAKQQPLRCLIYIEVALSVMSPTVPCPVP VCLSATQPTNLLPEPPRGSDVCLSLFPSRPCVCSLPKKLVLRDPNSSTLEISGPIRPI VSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA*" misc_feature complement(529..546) /label="Sp6_primer" misc_feature complement(560..578) /label="M13_reverse_primer" misc_feature complement(577..599) /label="M13_pUC_rev_primer" promoter complement(613..642) /label="lac_promoter" misc_feature complement(876..896) /label="pBABE_3_primer" misc_feature complement(882..1097) /label="SV40_enhancer" promoter 894..1162 /label="SV40_promoter" rep_origin 1061..1138 /label="SV40_origin" promoter 1014..1216 /label="SV40_promoter" misc_feature 1123..1142 /label="SV40pro_F_primer" CDS 1251..2045 /label="Neomycin" /gene="Neomycin" /note="ORF frame 3" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 1254..2042 /label="Neomycin" /gene="Neomycin" terminator 2080..2128 /label="synth_PA_terminator" rep_origin complement(2306..2925) /label="pBR322_origin" CDS complement(3080..3940) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3080..3940) /label="Ampicillin" /gene="Ampicillin" promoter complement(3982..4010) /label="AmpR_promoter" rep_origin complement(4255..4561) /label="f1_origin" misc_feature complement(4590..4738) /label="lacZ_a" misc_feature 4704..4726 /label="M13_pUC_fwd_primer" misc_feature 4719..4735 /label="M13_forward20_primer" promoter 4742..2 /label="T7_promoter" ORIGIN 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG GACCAGCTTC 61 TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG AGAGGCAGAC GTCACTTCCC 121 CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT TGAGTGGCAG AAAGGCAGAC GGGGACTGGG 181 CAAGGCACTG TCGGTGACAT CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA 241 GGCTGCTGCT TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG AAAGGGCTCG 361 GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG GCGTATGAGG CTGTGTCGGG 421 GCAGAGGCCC AAGATCTCGC AGTCTGGAGT TTCAAAAGTA GACTGGGCGG CCGCATCGAT 481 GTTAACCTCG AGGAGCTCCC AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC 541 CTAAATAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 661 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 721 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 781 CGCTCTTCCG CTGATCTGCG CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG 841 GTTAGGTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 901 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 961 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 1021 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 1081 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 1141 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 1201 CTTTTGCAAA AAGCTTGATT CTTCTGACGC TAGCGATCGC CCGGGCCACC ATGATTGAAC 1261 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT 1321 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC 1381 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG 1441 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 1501 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT 1561 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC 1621 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG 1681 CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG 1741 GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC 1801 TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT 1861 CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG 1921 CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT 1981 ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT 2041 TCTGACTCGA GTTTAAACTC TAGAACCGGT CATGGCCGCA ATAAAATATC TTTATTTTCA 2101 TTACATCTGT GTGTTGGTTT TTTGTGTGTT CGAACTAGAA GCTTGCTTCC TCGCTCACTG 2161 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 2221 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 2281 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 2341 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 2401 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 2461 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 2521 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 2581 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 2641 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 2701 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 2761 GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 2821 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 2881 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 2941 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 3001 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 3061 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 3121 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 3181 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 3241 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 3301 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 3361 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 3421 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 3481 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 3541 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 3601 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 3661 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 3721 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 3781 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 3841 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 3901 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 3961 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 4021 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GATGCGGTGT 4081 GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA AGCGTTAATA 4141 TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG 4201 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC 4261 CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA 4321 CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT 4381 CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC 4441 GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA 4501 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG 4561 CGCCGCTACA GGGCGCGTCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC 4621 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT 4681 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT 4741 GTAATACGAC TCACTATA // pGeneClip Neomycin "Times New Roman CORF 3"Times New RomanCɆT7_pro"Times New RomanC"Sp6_pri"Times New RomanCɆ0BM13_reverse_pri"Times New RomanAWM13_pUC_rev_pri"Times New Roman)Celac_pro"Times New RomanCl pBABE_3_pri"Times New RomanCɆrISV40_enh"Times New Roman)~SV40_pro"Times New Roman<CSV40_pro"Times New RomanC%r SV40_origin"Times New RomanQCɆcv SV40pro_F_pri"Times New Roman(ORF 3"Times New Roman*CNeomycin"Times New RomanC P synth_PA_term"Times New RomanCɆ m  pBR322_origin"Times New Roman dORF 2"Times New Roman*C d Ampicillin"Times New RomanCAmpR_pro"Times New RomanCɆ f1_origin"Times New RomanlacZ_a"Times New Roman)C`vM13_pUC_fwd_pri"Times New RomanCoM13_forward20_pri"Times New Roman :ApaI(14),EcoRV(18),SalI(28),EcoRI(34),BamHI(40),AatII(112)"Times New Roman =&r 2BglII(432),NotI(468),ClaI(476),HpaI(483),SacI(497)"Times New Roman4:??| KpnI"Times New RomanQvql]l  StuI(1194),NheI(1229),SmaI(1242)"Times New Roman  NarI(1379),PstI(1432),MscI(1461)"Times New Romanc- AgeI(2065),BstBI(2130)"Times New Roman #(BB test"Times New Romann%%=kZ test"Times New RomanEW2e$yy8d test"Times New RomanRjR