Sequence of pGeneClip

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Sequence of pGeneClip_Neomycin

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Sequence of pGeneClip Neomycin : 4758 BP; 1140 A; 1201 C; 1295 G; 1122 T; 0 other;

...... ApaI(14),EcoRV(18) SalI(28) EcoRI(34) ...... ..........

1 gggcgaattg ggcccgatat ctctagagtc gacgaattcg gatccctaag gaccagcttc 60

1 cccgcttaac ccgggctata gagatctcag ctgcttaagc ctagggattc ctggtcgaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... AatII(112)

61 tttgggagag aacagacgca ggggcgggag ggaaaaaggg agaggcagac gtcacttccc 120

61 aaaccctctc ttgtctgcgt ccccgccctc cctttttccc tctccgtctg cagtgaaggg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttggcggct ctggcagcag attggtcggt tgagtggcag aaaggcagac ggggactggg 180

121 gaaccgccga gaccgtcgtc taaccagcca actcaccgtc tttccgtctg cccctgaccc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaggcactg tcggtgacat cacggacagg gcgacttcta tgtagatgag gcagcgcaga 240

181 gttccgtgac agccactgta gtgcctgtcc cgctgaagat acatctactc cgtcgcgtct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggctgctgct tcgccacttg ctgcttcacc acgaaggagt tcccgtgccc tgggagcggg 300

241 ccgacgacga agcggtgaac gacgaagtgg tgcttcctca agggcacggg accctcgccc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttcaggaccg ctgatcggaa gtgagaatcc cagctgtgtg tcagggctgg aaagggctcg 360

301 aagtcctggc gactagcctt cactcttagg gtcgacacac agtcccgacc tttcccgagc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggagtgcgcg gggcaagtga ccgtgtgtgt aaagagtgag gcgtatgagg ctgtgtcggg 420

361 cctcacgcgc cccgttcact ggcacacaca tttctcactc cgcatactcc gacacagccc 420

.......... .......... .......... .......... .......... ..........

.......... BglII(432) .......... .......... NotI(468) ..........

421 gcagaggccc aagatctcgc agtctggagt ttcaaaagta gactgggcgg ccgcatcgat 480

421 cgtctccggg ttctagagcg tcagacctca aagttttcat ctgacccgcc ggcgtagcta 480

.......... .......... .......... .......... .......... ..........

HpaI(483) SacI(497) .......... .......... .......... ..........

481 gttaacctcg aggagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 540

481 caattggagc tcctcgaggg ttgcgcaacc tacgtatcga actcataaga tatcacagtg 540

.......... .......... .......... .......... .......... Sp6_pri

.......... .......... .......... .......... .......... ..........

541 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 600

541 gatttatcga accgcattag taccagtatc gacaaaggac acactttaac aataggcgag 600

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 660

601 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 660

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 720

661 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 780

721 agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg 840

781 gcgagaaggc gactagacgc gtcgtggtac cggactttat tggagacttt ctccttgaac 840

.......... .......... .......... .......... .......... ..........

KpnI(849) .......... .......... .......... .......... ..........

841 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 900

841 caatccatgg aagactccgc ctttcttggt cgacacctta cacacagtca atcccacacc 900

.......... .......... .......... ......... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 960

901 tttcaggggt ccgaggggtc gtccgtcttc atacgtttcg tacgtagagt taatcagtcg 960

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 1020

961 ttggtccaca cctttcaggg gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga 1020

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 1080

1021 gttaatcagt cgttggtatc agggcgggga ttgaggcggg tagggcgggg attgaggcgg 1080

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

1081 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 1140

1081 gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa aaataaatac gtctccggct 1140

SV40_enh SV40_pro SV40_pro SV40_origin ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

1141 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 1200

1141 ccggcggagc cggagactcg ataaggtctt catcactcct ccgaaaaaac ctccggatcc 1200

.......... SV40_pro SV40_pro .......... .......... ..........

.......... .......... NheI(1229) .......... SmaI(1242) ..........

1201 cttttgcaaa aagcttgatt cttctgacgc tagcgatcgc ccgggccacc atgattgaac 1260

1201 gaaaacgttt ttcgaactaa gaagactgcg atcgctagcg ggcccggtgg tactaacttg 1260

SV40_pro .......... .......... .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1261 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 1320

1261 ttctacctaa cgtgcgtcca agaggccggc gaacccacct ctccgataag ccgatactga 1320

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 1380

1321 cccgtgttgt ctgttagccg acgagactac ggcggcacaa ggccgacagt cgcgtccccg 1380

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 1440

1381 cgggccaaga aaaacagttc tggctggaca ggccacggga cttacttgac gtcctgctcc 1440

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... MscI(1461) .......... .......... ..........

1441 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 1500

1441 gtcgcgccga tagcaccgac cggtgctgcc cgcaaggaac gcgtcgacac gagctgcaac 1500

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 1560

1501 agtgacttcg cccttccctg accgacgata acccgcttca cggccccgtc ctagaggaca 1560

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 1620

1561 gtagagtgga acgaggacgg ctctttcata ggtagtaccg actacgttac gccgccgacg 1620

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 1680

1621 tatgcgaact aggccgatgg acgggtaagc tggtggttcg ctttgtagcg tagctcgctc 1680

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 1740

1681 gtgcatgagc ctaccttcgg ccagaacagc tagtcctact agacctgctt ctcgtagtcc 1740

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 1800

1741 ccgagcgcgg tcggcttgac aagcggtccg agttccgcgc gtacgggctg ccgctcctag 1800

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 1860

1801 agcagcactg ggtaccgcta cggacgaacg gcttatagta ccacctttta ccggcgaaaa 1860

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 1920

1861 gacctaagta gctgacaccg gccgacccac accgcctggc gatagtcctg tatcgcaacc 1920

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 1980

1921 gatgggcact ataacgactt ctcgaaccgc cgcttacccg actggcgaag gagcacgaaa 1980

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 2040

1981 tgccatagcg gcgagggcta agcgtcgcgt agcggaagat agcggaagaa ctgctcaaga 2040

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tctgactcga gtttaaactc tagaaccggt catggccgca ataaaatatc tttattttca 2100

2041 agactgagct caaatttgag atcttggcca gtaccggcgt tattttatag aaataaaagt 2100

.......... .......... .......... ........ synth_PA_term ........

.......... ......... BstBI(2130) ......... .......... ..........

2101 ttacatctgt gtgttggttt tttgtgtgtt cgaactagaa gcttgcttcc tcgctcactg 2160

2101 aatgtagaca cacaaccaaa aaacacacaa gcttgatctt cgaacgaagg agcgagtgac 2160

........ synth_PA_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 2220

2161 tgagcgacgc gagccagcaa gccgacgccg ctcgccatag tcgagtgagt ttccgccatt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 2280

2221 atgccaatag gtgtcttagt cccctattgc gtcctttctt gtacactcgt tttccggtcg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2340

2281 ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc gaggcggggg 2340

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2400

2341 gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc tgtcctgata 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2460

2401 tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa ggctgggacg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2520

2461 gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa agagtatcga 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2580

2521 gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg acacacgtgc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2640

2581 ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa ctcaggttgg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2700

2641 gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa tcgtctcgct 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2760

2701 ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg atgtgatctt 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2820

2761 cttgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt tctcaaccat 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2880

2821 cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa acgttcgtcg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2940

2881 tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga tgccccagac 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 3000

2941 tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtactctaat agtttttcct 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 3060

3001 agaagtggat ctaggaaaat ttaattttta cttcaaaatt tagttagatt tcatatatac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 3120

3061 tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga 3120

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3121 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3180

3121 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3240

3181 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3300

3241 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3360

3301 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3420

3361 gtcaattatc aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3480

3421 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3540

3481 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3600

3541 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3660

3601 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3720

3661 catacgccgc tggctcaacg agaacgggcc gcagttatgc cctattatgg cgcggtgtat 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3780

3721 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3840

3781 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3900

3841 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3960

3901 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 3960

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 4020

3961 taacttcgta aatagtccca ataacagagt actcgcctat gtataaactt acataaatct 4020

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt 4080

4021 ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt tcacggtgga ctacgccaca 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata 4140

4081 ctttatggcg tgtctacgca ttcctctttt atggcgtagt cctttaacat tcgcaattat 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 4200

4141 aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag taaaaaattg gttatccggc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 4260

4201 tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac tcacaacaag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 4320

4261 gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt cccgcttttt 4320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 4380

4321 ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gattagttca aaaaacccca 4380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 4440

4381 gctccacggc atttcgtgat ttagccttgg gatttccctc gggggctaaa tctcgaactg 4440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 4500

4441 cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct cgcccgcgat 4500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 4560

4501 cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg cgcgaattac 4560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc 4620

4561 gcggcgatgt cccgcgcagg taagcggtaa gtccgacgcg ttgacaaccc ttcccgctag 4620

.......... .......... .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

4621 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 4680

4621 ccacgcccgg agaagcgata atgcggtcga ccgctttccc cctacacgac gttccgctaa 4680

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 4740

4681 ttcaacccat tgcggtccca aaagggtcag tgctgcaaca ttttgctgcc ggtcacttaa 4740

.......... ... lacZ_a M13_pUC_fwd_pri M13_forward20_pri ...

.......... .......... .......... .......... .......... ..........

4741 gtaatacgac tcactata.. .......... .......... .......... .......... 4758

4741 cattatgctg agtgatat.. .......... .......... .......... .......... 4758

.......... .......... .......... .......... .......... ..........