Uk!Uk!7$LOCUS pGeneClip Puromycin 4561 bp DNA SYN 14-Sep-2007 DEFINITION pGeneClip Puromycin ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4561 /organism="pGeneClip Puromycin" /mol_type="other DNA" CDS complement(4393..476) /label="ORF frame 1" /translation="MRPPSLLLKLQTARSWASAPTQPHTPHSLHTRSLAPRTPEPFPA LTHSWDSHFRSAVLNPLPGHGNSFVVKQQVAKQQPLRCLIYIEVALSVMSPTVPCPVP VCLSATQPTNLLPEPPRGSDVCLSLFPSRPCVCSLPKKLVLRDPNSSTLEISGPIRPI VSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA*" misc_feature complement(529..546) /label="Sp6_primer" misc_feature complement(560..578) /label="M13_reverse_primer" misc_feature complement(577..599) /label="M13_pUC_rev_primer" promoter complement(613..642) /label="lac_promoter" misc_feature complement(876..896) /label="pBABE_3_primer" misc_feature complement(882..1097) /label="SV40_enhancer" promoter 894..1162 /label="SV40_promoter" rep_origin 1061..1138 /label="SV40_origin" promoter 1014..1216 /label="SV40_promoter" misc_feature 1123..1142 /label="SV40pro_F_primer" CDS 1239..1838 /label="puro(variant)" /gene="puro(variant)" /note="ORF frame 3" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" gene 1239..1838 /label="puro(variant)" /gene="puro(variant)" terminator 1883..1931 /label="synth_PA_terminator" rep_origin complement(2109..2728) /label="pBR322_origin" CDS complement(2883..3743) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(2883..3743) /label="Ampicillin" /gene="Ampicillin" promoter complement(3785..3813) /label="AmpR_promoter" rep_origin complement(4058..4364) /label="f1_origin" misc_feature complement(4393..4541) /label="lacZ_a" misc_feature 4507..4529 /label="M13_pUC_fwd_primer" misc_feature 4522..4538 /label="M13_forward20_primer" promoter 4545..2 /label="T7_promoter" ORIGIN 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG GACCAGCTTC 61 TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG AGAGGCAGAC GTCACTTCCC 121 CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT TGAGTGGCAG AAAGGCAGAC GGGGACTGGG 181 CAAGGCACTG TCGGTGACAT CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA 241 GGCTGCTGCT TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG AAAGGGCTCG 361 GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG GCGTATGAGG CTGTGTCGGG 421 GCAGAGGCCC AAGATCTCGC AGTCTGGAGT TTCAAAAGTA GACTGGGCGG CCGCATCGAT 481 GTTAACCTCG AGGAGCTCCC AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC 541 CTAAATAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 661 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 721 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 781 CGCTCTTCCG CTGATCTGCG CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG 841 GTTAGGTACC TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 901 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 961 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 1021 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 1081 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 1141 GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 1201 CTTTTGCAAA AAGCTTGATT CTTCTGACGC TAGCCACCAT GACCGAGTAC AAGCCCACGG 1261 TGCGCCTCGC CACCCGCGAC GACGTCCCCC GGGCCGTACG CACCCTCGCC GCCGCGTTCG 1321 CCGACTACCC CGCCACGCGC CACACCGTCG ACCCGGACCG CCACATCGAG CGGGTCACCG 1381 AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG TGGGTCGCGG 1441 ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG GGGGCGGTGT 1501 TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC GCGCAGCAAC 1561 AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC CTGGCCACCG 1621 TCGGCGTGTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG CTCCCCGGAG 1681 TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG CCCCGCAACC 1741 TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG CCCGAAGGAC 1801 CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACT GCAGGATCCT CGAGTTTAAA 1861 CTCTAGAACC GGTCATGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG 1921 TTTTTTGTGT GTTCGAACTA GAAGCTTGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 1981 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 2041 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 2101 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 2161 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 2221 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 2281 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 2341 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 2401 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 2461 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 2521 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 2581 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 2641 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA 2701 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA 2761 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT 2821 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 2881 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC 2941 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC 3001 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA 3061 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC 3121 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 3181 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA 3241 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA 3301 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA 3361 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT 3421 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 3481 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG 3541 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA 3601 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC 3661 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG 3721 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 3781 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 3841 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGATGCGG TGTGAAATAC CGCACAGATG 3901 CGTAAGGAGA AAATACCGCA TCAGGAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG 3961 TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT 4021 TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT 4081 CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT 4141 GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA 4201 CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC 4261 GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA 4321 GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG 4381 TCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC 4441 TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG 4501 GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTGTAATAC GACTCACTAT 4561 A //pGeneClip Puromycin "Times New Roman!Uk!C)ORF 1"Times New RomanUk!CɆT7_pro"Times New RomanUk!C"Sp6_pri"Times New RomanUk!CɆ0BM13_reverse_pri"Times New RomanUk!AWM13_pUC_rev_pri"Times New Roman)Uk!Celac_pro"Times New RomanUk!Cl pBABE_3_pri"Times New RomanUk!CɆrISV40_enh"Times New Roman(Uk!~SV40_pro"Times New Roman=Uk!CSV40_pro"Times New RomanUk!C%r SV40_origin"Times New RomanPUk!CɆcv SV40pro_F_pri"Times New Roman(Uk!.ORF 3"Times New Roman*Uk!C. puro(variant)"Times New RomanUk!C[ synth_PA_term"Times New RomanUk!CɆ=  pBR322_origin"Times New RomanUk!C ORF 3"Times New RomanUk!CC  Ampicillin"Times New Roman(Uk!CAmpR_pro"Times New RomanUk!CɆ  f1_origin"Times New RomanUk!)lacZ_a"Times New Roman)Uk!CM13_pUC_fwd_pri"Times New RomanUk!CM13_forward20_pri"Times New Roman Uk!ApaI(14),EcoRV(18),EcoRI(34)"Times New Roman PWUKAAA Uk!2BglII(432),NotI(468),ClaI(476),HpaI(483),SacI(497)"Times New Roman| tk Mk Uk!KpnI"Times New RomanQXajj Uk!NheI(1229),SmaI(1290)"Times New RomaniWsX|Y|Jh Uk!SacII"Times New RomanW`ii Uk!MscI"Times New RomanN<DKK Uk!!PstI(1843),AgeI(1868),BstBI(1933)"Times New Roman3 22y0 Uk!test"Times New Roman|U_wnn Uk!test"Times New RomanS*8NNa Uk!test"Times New RomanhK#r0