Sequence of pGeneClip

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Sequence of pGeneClip_Puromycin

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Sequence of pGeneClip Puromycin : 4561 BP; 1083 A; 1209 C; 1249 G; 1020 T; 0 other;

...... ApaI(14),EcoRV(18) ...... EcoRI(34) .......... ..........

1 gggcgaattg ggcccgatat ctctagagtc gacgaattcg gatccctaag gaccagcttc 60

1 cccgcttaac ccgggctata gagatctcag ctgcttaagc ctagggattc ctggtcgaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tttgggagag aacagacgca ggggcgggag ggaaaaaggg agaggcagac gtcacttccc 120

61 aaaccctctc ttgtctgcgt ccccgccctc cctttttccc tctccgtctg cagtgaaggg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttggcggct ctggcagcag attggtcggt tgagtggcag aaaggcagac ggggactggg 180

121 gaaccgccga gaccgtcgtc taaccagcca actcaccgtc tttccgtctg cccctgaccc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaggcactg tcggtgacat cacggacagg gcgacttcta tgtagatgag gcagcgcaga 240

181 gttccgtgac agccactgta gtgcctgtcc cgctgaagat acatctactc cgtcgcgtct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggctgctgct tcgccacttg ctgcttcacc acgaaggagt tcccgtgccc tgggagcggg 300

241 ccgacgacga agcggtgaac gacgaagtgg tgcttcctca agggcacggg accctcgccc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttcaggaccg ctgatcggaa gtgagaatcc cagctgtgtg tcagggctgg aaagggctcg 360

301 aagtcctggc gactagcctt cactcttagg gtcgacacac agtcccgacc tttcccgagc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggagtgcgcg gggcaagtga ccgtgtgtgt aaagagtgag gcgtatgagg ctgtgtcggg 420

361 cctcacgcgc cccgttcact ggcacacaca tttctcactc cgcatactcc gacacagccc 420

.......... .......... .......... .......... .......... ..........

.......... BglII(432) .......... .......... NotI(468) ..........

421 gcagaggccc aagatctcgc agtctggagt ttcaaaagta gactgggcgg ccgcatcgat 480

421 cgtctccggg ttctagagcg tcagacctca aagttttcat ctgacccgcc ggcgtagcta 480

.......... .......... .......... .......... .......... ..........

HpaI(483) SacI(497) .......... .......... .......... ..........

481 gttaacctcg aggagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 540

481 caattggagc tcctcgaggg ttgcgcaacc tacgtatcga actcataaga tatcacagtg 540

.......... .......... .......... .......... .......... Sp6_pri

.......... .......... .......... .......... .......... ..........

541 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 600

541 gatttatcga accgcattag taccagtatc gacaaaggac acactttaac aataggcgag 600

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 660

601 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 660

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 720

661 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 780

721 agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg 840

781 gcgagaaggc gactagacgc gtcgtggtac cggactttat tggagacttt ctccttgaac 840

.......... .......... .......... .......... .......... ..........

KpnI(849) .......... .......... .......... .......... ..........

841 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg 900

841 caatccatgg aagactccgc ctttcttggt cgacacctta cacacagtca atcccacacc 900

.......... .......... .......... ......... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

901 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 960

901 tttcaggggt ccgaggggtc gtccgtcttc atacgtttcg tacgtagagt taatcagtcg 960

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 1020

961 ttggtccaca cctttcaggg gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga 1020

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 1080

1021 gttaatcagt cgttggtatc agggcgggga ttgaggcggg tagggcgggg attgaggcgg 1080

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

1081 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 1140

1081 gtcaaggcgg gtaagaggcg gggtaccgac tgattaaaaa aaataaatac gtctccggct 1140

SV40_enh SV40_pro SV40_pro SV40_origin ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

1141 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 1200

1141 ccggcggagc cggagactcg ataaggtctt catcactcct ccgaaaaaac ctccggatcc 1200

.......... SV40_pro SV40_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cttttgcaaa aagcttgatt cttctgacgc tagccaccat gaccgagtac aagcccacgg 1260

1201 gaaaacgttt ttcgaactaa gaagactgcg atcggtggta ctggctcatg ttcgggtgcc 1260

SV40_pro .......... .......... ..... ORF 3 puro(variant) .....

.......... .......... SmaI(1290) .......... .......... ..........

1261 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg 1320

1261 acgcggagcg gtgggcgctg ctgcaggggg cccggcatgc gtgggagcgg cggcgcaagc 1320

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg 1380

1321 ggctgatggg gcggtgcgcg gtgtggcagc tgggcctggc ggtgtagctc gcccagtggc 1380

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 1440

1381 tcgacgttct tgagaaggag tgcgcgcagc ccgagctgta gccgttccac acccagcgcc 1440

.......... ..... ORF 3 puro(variant) ..... .......... ..........

......... SacII(1453) ......... .......... .......... ..........

1441 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 1500

1441 tgctgccgcg gcgccaccgc cagacctggt gcggcctctc gcagcttcgc ccccgccaca 1500

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 1560

1501 agcggctcta gccgggcgcg taccggctca actcgccaag ggccgaccgg cgcgtcgttg 1560

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... MscI(1614)

1561 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 1620

1561 tctaccttcc ggaggaccgc ggcgtggccg ggttcctcgg gcgcaccaag gaccggtggc 1620

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcggcgtgtc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 1680

1621 agccgcacag cgggctggtg gtcccgttcc cagacccgtc gcggcagcac gaggggcctc 1680

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 1740

1681 acctccgccg gctcgcgcgg ccccacgggc ggaaggacct ctggaggcgc ggggcgttgg 1740

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 1800

1741 aggggaagat gctcgccgag ccgaagtggc agtggcggct gcagctccac gggcttcctg 1800

.......... ..... ORF 3 puro(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgact gcaggatcct cgagtttaaa 1860

1801 gcgcgtggac cacgtactgg gcgttcgggc cacggactga cgtcctagga gctcaaattt 1860

..... ORF 3 puro(variant) ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctctagaacc ggtcatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg 1920

1861 gagatcttgg ccagtaccgg cgttatttta tagaaataaa agtaatgtag acacacaacc 1920

.......... .......... .......... ........ synth_PA_term ........

......... BstBI(1933) ......... .......... .......... ..........

1921 ttttttgtgt gttcgaacta gaagcttgct tcctcgctca ctgactcgct gcgctcggtc 1980

1921 aaaaaacaca caagcttgat cttcgaacga aggagcgagt gactgagcga cgcgagccag 1980

synth_PA_term ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 2040

1981 caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 2100

2041 agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 2160

2101 tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt 2160

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 2220

2161 ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 2280

2221 gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 2340

2281 aggcggaaag agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 2400

2341 tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 2460

2401 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 2520

2461 agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 2580

2521 tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcttgtca taaaccatag 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 2640

2581 acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 2700

2641 gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 2760

2701 tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt 2760

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 2820

2761 ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2880

2821 aatttaattt ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2940

2881 tcaatggtta cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg 2940

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 3000

2941 tatcaacgga ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 3060

3001 gggtcacgac gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 3120

3061 ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 3180

3121 gtcagataat taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3240

3181 ttgcaacaac ggtaacgatg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3300

3241 aagtcgaggc caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3360

3301 cgccaatcga ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3420

3361 gagtaccaat accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3480

3421 agacactgac cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 3540

3481 acgagaacgg gccgcagtta tgccctatta tggcgcggtg tatcgtcttg aaattttcac 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3600

3541 gagtagtaac cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 3660

3601 aggtcaagct acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 3720

3661 tcgcaaagac ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3780

3721 tgtgccttta caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc 3780

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3840

3781 ccaataacag agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc 3840

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gttccgcgca catttccccg aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg 3900

3841 caaggcgcgt gtaaaggggc ttttcacggt ggactacgcc acactttatg gcgtgtctac 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg 3960

3901 gcattcctct tttatggcgt agtcctttaa cattcgcaat tataaaacaa ttttaagcgc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct 4020

3961 aatttaaaaa caatttagtc gagtaaaaaa ttggttatcc ggctttagcc gttttaggga 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt 4080

4021 atatttagtt ttcttatctg gctctatccc aactcacaac aaggtcaaac cttgttctca 4080

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

4081 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat 4140

4081 ggtgataatt tcttgcacct gaggttgcag tttcccgctt tttggcagat agtcccgcta 4140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca 4200

4141 ccgggtgatg cacttggtag tgggattagt tcaaaaaacc ccagctccac ggcatttcgt 4200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac 4260

4201 gatttagcct tgggatttcc ctcgggggct aaatctcgaa ctgccccttt cggccgcttg 4260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta 4320

4261 caccgctctt tccttccctt ctttcgcttt cctcgcccgc gatcccgcga ccgttcacat 4320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg 4380

4321 cgccagtgcg acgcgcattg gtggtgtggg cggcgcgaat tacgcggcga tgtcccgcgc 4380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc 4440

4381 aggtaagcgg taagtccgac gcgttgacaa cccttcccgc tagccacgcc cggagaagcg 4440

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag 4500

4441 ataatgcggt cgaccgcttt ccccctacac gacgttccgc taattcaacc cattgcggtc 4500

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat 4560

4501 ccaaaagggt cagtgctgca acattttgct gccggtcact taacattatg ctgagtgata 4560

M13_pUC_fwd_pri lacZ_a M13_forward20_pri .......... ..........

.......... .......... .......... .......... .......... ..........

4561 a......... .......... .......... .......... .......... .......... 4561

4561 t......... .......... .......... .......... .......... .......... 4561

.......... .......... .......... .......... .......... ..........