qq%'LOCUS pGeneClip hMGFP 5267 bp DNA SYN 28-Aug-2007 DEFINITION pGeneClip hMGFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5267 /organism="pGeneClip hMGFP" /mol_type="other DNA" CDS complement(5099..476) /label="ORF frame 2" /translation="MRPPSLLLKLQTARSWASAPTQPHTPHSLHTRSLAPRTPEPFPA LTHSWDSHFRSAVLNPLPGHGNSFVVKQQVAKQQPLRCLIYIEVALSVMSPTVPCPVP VCLSATQPTNLLPEPPRGSDVCLSLFPSRPCVCSLPKKLVLRDPNSSTLEISGPIRPI VSRITIHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAA*" misc_feature complement(529..546) /label="Sp6_primer" misc_feature complement(560..578) /label="M13_reverse_primer" misc_feature complement(577..599) /label="M13_pUC_rev_primer" promoter complement(613..642) /label="lac_promoter" misc_feature 1018..1305 /label="CAG_enhancer" promoter 963..1523 /label="CMV_immearly_promoter" misc_feature 1480..1500 /label="CMV_fwd_primer" promoter 1469..1550 /label="CMV_promoter" misc_feature 1524..1543 /label="pCEP_fwd_primer" misc_feature 1526..1550 /label="LNCX_primer" misc_feature 1690..1822 /label="chim_int" CDS 1880..2563 /label="ORF frame 2" /translation="MGVIKPDMKIKLRMEGAVNGHKFVIEGDGKGKPFEGKQTMDLTV IEGAPLPFAYDILTTVFDYGNRVFAKYPKDIPDYFKQTFPEGYSWERSMTYEDQGICI ATNDITMMKGVDDCFVYKIRFDGVNFPANGPVMQRKTLKWEPSTEKMYVRDGVLKGDV NMALLLEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHRIEIVSHDKDYNKVKLYEHAEA HSGLPRQAG*" terminator 2589..2637 /label="synth_PA_terminator" rep_origin complement(2815..3434) /label="pBR322_origin" CDS complement(3589..4449) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3589..4449) /label="Ampicillin" /gene="Ampicillin" promoter complement(4491..4519) /label="AmpR_promoter" rep_origin complement(4764..5070) /label="f1_origin" misc_feature complement(5099..5247) /label="lacZ_a" misc_feature 5213..5235 /label="M13_pUC_fwd_primer" misc_feature 5228..5244 /label="M13_forward20_primer" promoter 5251..2 /label="T7_promoter" ORIGIN 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG GACCAGCTTC 61 TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG AGAGGCAGAC GTCACTTCCC 121 CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT TGAGTGGCAG AAAGGCAGAC GGGGACTGGG 181 CAAGGCACTG TCGGTGACAT CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA 241 GGCTGCTGCT TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG AAAGGGCTCG 361 GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG GCGTATGAGG CTGTGTCGGG 421 GCAGAGGCCC AAGATCTCGC AGTCTGGAGT TTCAAAAGTA GACTGGGCGG CCGCATCGAT 481 GTTAACCTCG AGGAGCTCCC AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC 541 CTAAATAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 661 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 721 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG 781 CGCTCTTCCG CTCTTCCGCT TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA 841 GCATAAATCA ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 901 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT GACTAGTTAT 961 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA 1021 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 1081 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 1141 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 1201 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 1261 TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 1321 ATGCGGTTTT GGCAGTACAC CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 1381 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 1441 CCAAAATGTC GTAACAACTG CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG 1501 TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC ACTAGAAGCT 1561 TTATTGCGGT AGTTTATCAC AGTTAAATTG CTAACGCAGT CAGTGCTTCT GACACAACAG 1621 TCTCGAACTT AAGCTGCAGT GACTCTCTTA AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC 1681 ACTGGGCAGG TAAGTATCAA GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC 1741 TTGTCGAGAC AGAGAAGACT CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC 1801 CACTTTGCCT TTCTCTCCAC AGGTGTCCAC TCCCAGTTCA ATTACAGCTC GCTAGCGATC 1861 GCCCGGGGAT ATCGCCACCA TGGGCGTGAT CAAGCCCGAC ATGAAGATCA AGCTGCGGAT 1921 GGAGGGCGCC GTGAACGGCC ACAAATTCGT GATCGAGGGC GACGGGAAAG GCAAGCCCTT 1981 TGAGGGTAAG CAGACTATGG ACCTGACCGT GATCGAGGGC GCCCCCCTGC CCTTCGCTTA 2041 TGACATTCTC ACCACCGTGT TCGACTACGG TAACCGTGTC TTCGCCAAGT ACCCCAAGGA 2101 CATCCCTGAC TACTTCAAGC AGACCTTCCC CGAGGGCTAC TCGTGGGAGC GAAGCATGAC 2161 ATACGAGGAC CAGGGAATCT GTATCGCTAC AAACGACATC ACCATGATGA AGGGTGTGGA 2221 CGACTGCTTC GTGTACAAAA TCCGCTTCGA CGGGGTCAAC TTCCCTGCTA ATGGCCCGGT 2281 GATGCAGCGC AAGACCCTAA AGTGGGAGCC CAGTACCGAG AAGATGTACG TGCGGGACGG 2341 CGTACTGAAG GGCGATGTTA ATATGGCACT GCTCTTGGAG GGAGGCGGCC ACTACCGCTG 2401 CGACTTCAAG ACCACCTACA AAGCCAAGAA GGTGGTGCAG CTTCCCGACT ACCACTTCGT 2461 GGACCACCGC ATCGAGATCG TGAGCCACGA CAAGGACTAC AACAAAGTCA AGCTGTACGA 2521 GCACGCCGAA GCCCACAGCG GACTACCCCG CCAGGCCGGC TAATAGTTCT AGAACCGGTC 2581 ATGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGTTC 2641 GAACTAGAAG CTTGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 2701 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 2761 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 2821 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 2881 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 2941 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 3001 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 3061 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 3121 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 3181 AGCCACTGGT AACGGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 3241 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 3301 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 3361 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 3421 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 3481 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 3541 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 3601 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 3661 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 3721 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 3781 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 3841 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 3901 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 3961 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 4021 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 4081 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 4141 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 4201 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 4261 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 4321 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 4381 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 4441 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 4501 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 4561 TCCCCGAAAA GTGCCACCTG ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT 4621 ACCGCATCAG GAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA 4681 AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA 4741 ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA 4801 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA 4861 ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC 4921 TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA 4981 AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG 5041 CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCA TTCGCCATTC 5101 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 5161 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 5221 CGACGTTGTA AAACGACGGC CAGTGAATTG TAATACGACT CACTATA //pGeneClip hMGFP "Times New Roman!qCORF 2"Times New RomanqCɆT7_pro"Times New RomanqC"Sp6_pri"Times New RomanqCɆ0BM13_reverse_pri"Times New RomanqAWM13_pUC_rev_pri"Times New RomanqCelac_pro"Times New RomanqCɆCMV_immearly_pro"Times New Roman(qCCAG_enh"Times New RomanqCCMV_pro"Times New Romanq CMV_fwd_pri"Times New RomanqC pCEP_fwd_pri"Times New RomanqCɆLNCX_pri"Times New Roman*qchim_int"Times New RomanqCX ORF 2"Times New RomanqC M  synth_PA_term"Times New RomanqCɆ j  pBR322_origin"Times New RomanqaORF 1"Times New Roman(qCa Ampicillin"Times New RomanqCAmpR_pro"Times New RomanqCɆ f1_origin"Times New RomanqlacZ_a"Times New RomanqC]sM13_pUC_fwd_pri"Times New RomanqCl|M13_forward20_pri"Times New Roman* q%ApaI(14),SalI(28),EcoRI(34),BamHI(40)"Times New Roman =9FL q