Sequence of pGeneClip

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pGeneClip_hMGFP

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pGeneClip hMGFP : 5267 BP; 1333 A; 1296 C; 1374 G; 1264 T; 0 other;

..... ApaI(14) SalI(28) EcoRI(34) BamHI(40) ..... ..........

1 gggcgaattg ggcccgatat ctctagagtc gacgaattcg gatccctaag gaccagcttc 60

1 cccgcttaac ccgggctata gagatctcag ctgcttaagc ctagggattc ctggtcgaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tttgggagag aacagacgca ggggcgggag ggaaaaaggg agaggcagac gtcacttccc 120

61 aaaccctctc ttgtctgcgt ccccgccctc cctttttccc tctccgtctg cagtgaaggg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttggcggct ctggcagcag attggtcggt tgagtggcag aaaggcagac ggggactggg 180

121 gaaccgccga gaccgtcgtc taaccagcca actcaccgtc tttccgtctg cccctgaccc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caaggcactg tcggtgacat cacggacagg gcgacttcta tgtagatgag gcagcgcaga 240

181 gttccgtgac agccactgta gtgcctgtcc cgctgaagat acatctactc cgtcgcgtct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggctgctgct tcgccacttg ctgcttcacc acgaaggagt tcccgtgccc tgggagcggg 300

241 ccgacgacga agcggtgaac gacgaagtgg tgcttcctca agggcacggg accctcgccc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ttcaggaccg ctgatcggaa gtgagaatcc cagctgtgtg tcagggctgg aaagggctcg 360

301 aagtcctggc gactagcctt cactcttagg gtcgacacac agtcccgacc tttcccgagc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggagtgcgcg gggcaagtga ccgtgtgtgt aaagagtgag gcgtatgagg ctgtgtcggg 420

361 cctcacgcgc cccgttcact ggcacacaca tttctcactc cgcatactcc gacacagccc 420

.......... .......... .......... .......... .......... ..........

.......... BglII(432) .......... ..... NotI(468),EagI(468) .....

421 gcagaggccc aagatctcgc agtctggagt ttcaaaagta gactgggcgg ccgcatcgat 480

421 cgtctccggg ttctagagcg tcagacctca aagttttcat ctgacccgcc ggcgtagcta 480

.......... .......... .......... .......... .......... ..........

HpaI(483),XhoI(487) .......... .......... .......... ..........

481 gttaacctcg aggagctccc aacgcgttgg atgcatagct tgagtattct atagtgtcac 540

481 caattggagc tcctcgaggg ttgcgcaacc tacgtatcga actcataaga tatcacagtg 540

.......... .......... .......... .......... .......... Sp6_pri

.......... .......... .......... .......... .......... ..........

541 ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 600

541 gatttatcga accgcattag taccagtatc gacaaaggac acactttaac aataggcgag 600

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

601 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 660

601 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 660

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 720

661 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 780

721 agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgctcttccg ctcttccgct tcaatattgg ccattagcca tattattcat tggttatata 840

781 gcgagaaggc gagaaggcga agttataacc ggtaatcggt ataataagta accaatatat 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac 900

841 cgtatttagt tataaccgat aaccggtaac gtatgcaaca tagatatagt attatacatg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SpeI(952)

901 atttatattg gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat 960

901 taaatataac cgagtacagg ttatactggc ggtacaaccg taactaataa ctgatcaata 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 1020

961 attatcatta gttaatgccc cagtaatcaa gtatcgggta tatacctcaa ggcgcaatgt 1020

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

1021 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 1080

1021 attgaatgcc atttaccggg cggaccgact ggcgggttgc tgggggcggg taactgcagt 1080

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 1140

1081 tattactgca tacaagggta tcattgcggt tatccctgaa aggtaactgc agttacccac 1140

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... NdeI(1187) ..........

1141 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg 1200

1141 ctcataaatg ccatttgacg ggtgaaccgt catgtagttc acatagtata cggttcaggc 1200

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 1260

1201 gggggataac tgcagttact gccatttacc gggcggaccg taatacgggt catgtactgg 1260

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 1320

1261 aatgccctga aaggatgaac cgtcatgtag atgcataatc agtagcgata atggtaccac 1320

.......... ... CMV_immearly_pro CAG_enh ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca 1380

1321 tacgccaaaa ccgtcatgtg gttacccgca cctatcgcca aactgagtgc ccctaaaggt 1380

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 1440

1381 tcagaggtgg ggtaactgca gttaccctca aacaaaaccg tggttttagt tgccctgaaa 1440

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccaaaatgtc gtaacaactg cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg 1500

1441 ggttttacag cattgttgac gctagcgggc ggggcaactg cgtttacccg ccatccgcac 1500

.......... ....... CMV_immearly_pro .. CMV_pro CMV_fwd_pri .....

.......... .......... .......... .......... .......... ..........

1501 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc actagaagct 1560

1501 atgccaccct ccagatatat tcgtctcgag caaatcactt ggcagtctag tgatcttcga 1560

. CMV_immearly_pro CMV_pro pCEP_fwd_pri LNCX_pri . ..........

.......... .......... .......... .......... .......... ..........

1561 ttattgcggt agtttatcac agttaaattg ctaacgcagt cagtgcttct gacacaacag 1620

1561 aataacgcca tcaaatagtg tcaatttaac gattgcgtca gtcacgaaga ctgtgttgtc 1620

.......... .......... .......... .......... .......... ..........

.......... PstI(1638) .......... .......... .......... ..........

1621 tctcgaactt aagctgcagt gactctctta aggtagcctt gcagaagttg gtcgtgaggc 1680

1621 agagcttgaa ttcgacgtca ctgagagaat tccatcggaa cgtcttcaac cagcactccg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc 1740

1681 tgacccgtcc attcatagtt ccaatgttct gtccaaattc ctctggttat ctttgacccg 1740

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc 1800

1741 aacagctctg tctcttctga gaacgcaaag actatccgtg gataaccaga atgactgtag 1800

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc gctagcgatc 1860

1801 gtgaaacgga aagagaggtg tccacaggtg agggtcaagt taatgtcgag cgatcgctag 1860

.......... chim_int .......... .......... .......... ..........

SmaI(1864) .......... BclI(1887) .......... .......... ..........

1861 gcccggggat atcgccacca tgggcgtgat caagcccgac atgaagatca agctgcggat 1920

1861 cgggccccta tagcggtggt acccgcacta gttcgggctg tacttctagt tcgacgccta 1920

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggagggcgcc gtgaacggcc acaaattcgt gatcgagggc gacgggaaag gcaagccctt 1980

1921 cctcccgcgg cacttgccgg tgtttaagca ctagctcccg ctgccctttc cgttcgggaa 1980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgagggtaag cagactatgg acctgaccgt gatcgagggc gcccccctgc ccttcgctta 2040

1981 actcccattc gtctgatacc tggactggca ctagctcccg cggggggacg ggaagcgaat 2040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tgacattctc accaccgtgt tcgactacgg taaccgtgtc ttcgccaagt accccaagga 2100

2041 actgtaagag tggtggcaca agctgatgcc attggcacag aagcggttca tggggttcct 2100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catccctgac tacttcaagc agaccttccc cgagggctac tcgtgggagc gaagcatgac 2160

2101 gtagggactg atgaagttcg tctggaaggg gctcccgatg agcaccctcg cttcgtactg 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atacgaggac cagggaatct gtatcgctac aaacgacatc accatgatga agggtgtgga 2220

2161 tatgctcctg gtcccttaga catagcgatg tttgctgtag tggtactact tcccacacct 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgactgcttc gtgtacaaaa tccgcttcga cggggtcaac ttccctgcta atggcccggt 2280

2221 gctgacgaag cacatgtttt aggcgaagct gccccagttg aagggacgat taccgggcca 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gatgcagcgc aagaccctaa agtgggagcc cagtaccgag aagatgtacg tgcgggacgg 2340

2281 ctacgtcgcg ttctgggatt tcaccctcgg gtcatggctc ttctacatgc acgccctgcc 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgtactgaag ggcgatgtta atatggcact gctcttggag ggaggcggcc actaccgctg 2400

2341 gcatgacttc ccgctacaat tataccgtga cgagaacctc cctccgccgg tgatggcgac 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgacttcaag accacctaca aagccaagaa ggtggtgcag cttcccgact accacttcgt 2460

2401 gctgaagttc tggtggatgt ttcggttctt ccaccacgtc gaagggctga tggtgaagca 2460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggaccaccgc atcgagatcg tgagccacga caaggactac aacaaagtca agctgtacga 2520

2461 cctggtggcg tagctctagc actcggtgct gttcctgatg ttgtttcagt tcgacatgct 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcacgccgaa gcccacagcg gactaccccg ccaggccggc taatagttct agaaccggtc 2580

2521 cgtgcggctt cgggtgtcgc ctgatggggc ggtccggccg attatcaaga tcttggccag 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... ......... BstBI(2639)

2581 atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc 2640

2581 taccggcgtt attttataga aataaaagta atgtagacac acaaccaaaa aacacacaag 2640

.......... .......... ........ synth_PA_term ........ ..........

.......... .......... .......... .......... .......... ..........

2641 gaactagaag cttgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 2700

2641 cttgatcttc gaacgaagga gcgagtgact gagcgacgcg agccagcaag ccgacgccgc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 2760

2701 tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc ccctattgcg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 2820

2761 tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 2880

2821 cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag ctgcgagttc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 2940

2881 agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg gaccttcgag 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3000

2941 ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc ggaaagaggg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 3060

3001 aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc atagagtcaa gccacatcca 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3120

3061 gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3180

3121 taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agccactggt aacgggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 3240

3181 tcggtgacca ttgccctaat cgtctcgctc catacatccg ccacgatgtc tcaagaactt 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 3300

3241 caccaccgga ttgatgccga tgtgatcttc ttgtcataaa ccatagacgc gagacgactt 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 3360

3301 cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 3420

3361 atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 3480

3421 tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc 3480

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 3540

3481 ctaaaaccag tactctaata gtttttccta gaagtggatc taggaaaatt taatttttac 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 3600

3541 ttcaaaattt agttagattt catatatact catttgaacc agactgtcaa tggttacgaa 3600

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

3601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 3660

3601 ttagtcactc cgtggataga gtcgctagac agataaagca agtaggtatc aacggactga 3660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 3720

3661 ggggcagcac atctattgat gctatgccct cccgaatggt agaccggggt cacgacgtta 3720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 3780

3721 ctatggcgct ctgggtgcga gtggccgagg tctaaatagt cgttatttgg tcggtcggcc 3780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 3840

3781 ttcccggctc gcgtcttcac caggacgttg aaataggcgg aggtaggtca gataattaac 3840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 3900

3841 aacggccctt cgatctcatt catcaagcgg tcaattatca aacgcgttgc aacaacggta 3900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 3960

3901 acgatgtccg tagcaccaca gtgcgagcag caaaccatac cgaagtaagt cgaggccaag 3960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4020

3961 ggttgctagt tccgctcaat gtactagggg gtacaacacg ttttttcgcc aatcgaggaa 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4080

4021 gccaggaggc tagcaacagt cttcattcaa ccggcgtcac aatagtgagt accaataccg 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4140

4081 tcgtgacgta ttaagagaat gacagtacgg taggcattct acgaaaagac actgaccact 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4200

4141 catgagttgg ttcagtaaga ctcttatcac atacgccgct ggctcaacga gaacgggccg 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4260

4201 cagttatgcc ctattatggc gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 4320

4261 tgcaagaagc cccgcttttg agagttccta gaatggcgac aactctaggt caagctacat 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 4380

4321 tgggtgagca cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc aaagacccac 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 4440

4381 tcgtttttgt ccttccgttt tacggcgttt tttcccttat tcccgctgtg cctttacaac 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 4500

4441 ttatgagtat gagaaggaaa aagttataat aacttcgtaa atagtcccaa taacagagta 4500

ORF 1 Ampicillin .... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

4501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 4560

4501 ctcgcctatg tataaactta cataaatctt tttatttgtt tatccccaag gcgcgtgtaa 4560

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta aggagaaaat 4620

4561 aggggctttt cacggtggac tacgccacac tttatggcgt gtctacgcat tcctctttta 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta 4680

4621 tggcgtagtc ctttaacatt cgcaattata aaacaatttt aagcgcaatt taaaaacaat 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 4740

4681 ttagtcgagt aaaaaattgg ttatccggct ttagccgttt tagggaatat ttagttttct 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 4800

4741 tatctggctc tatcccaact cacaacaagg tcaaaccttg ttctcaggtg ataatttctt 4800

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

4801 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 4860

4801 gcacctgagg ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg gtgatgcact 4860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 4920

4861 tggtagtggg attagttcaa aaaaccccag ctccacggca tttcgtgatt tagccttggg 4920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 4980

4921 atttccctcg ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc gctctttcct 4980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 5040

4981 tcccttcttt cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc agtgcgacgc 5040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc 5100

5041 gcattggtgg tgtgggcggc gcgaattacg cggcgatgtc ccgcgcaggt aagcggtaag 5100

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 5160

5101 tccgacgcgt tgacaaccct tcccgctagc cacgcccgga gaagcgataa tgcggtcgac 5160

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 5220

5161 cgctttcccc ctacacgacg ttccgctaat tcaacccatt gcggtcccaa aagggtcagt 5220

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgacgttgta aaacgacggc cagtgaattg taatacgact cactata... .......... 5267

5221 gctgcaacat tttgctgccg gtcacttaac attatgctga gtgatat... .......... 5267

M13_pUC_fwd_pri lacZ_a M13_forward20_pri .......... ..........