Sequence of pGene_V5-His_A

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Sequence of pGene_V5-His_A_(GeneSwitch)

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Sequence of pGene/V5-His A (GeneSwitch) : 4654 BP; 1052 A; 1239 C; 1192 G; 1171 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ccgagctctt acgcgggtcg aagcggagta ctgtcctccg agtggagtac tgtcctccga 60

1 ggctcgagaa tgcgcccagc ttcgcctcat gacaggaggc tcacctcatg acaggaggct 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcggagtact gtcctccgag tcgagggtcg aagcggagta ctgtcctccg agtggagtac 120

61 cgcctcatga caggaggctc agctcccagc ttcgcctcat gacaggaggc tcacctcatg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgtcctccga gcggagtact gtcctccgag tcgactctag agggtatata atggatctcg 180

121 acaggaggct cgcctcatga caggaggctc agctgagatc tcccatatat tacctagagc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agatatcgga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt 240

181 tctatagcct cgagcaaatc acttggcagt ctagcggacc tctgcggtag gtgcgacaaa 240

.......... .......... LNCX_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg 300

241 actggaggta tcttctgtgg ccctggctag gtcggaggcg ccggcccttg ccacgtaacc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aacgcgcatt ccccgtgtta attaacaggt aagtgtcttc ctcctgtttc cttcccctgc 360

301 ttgcgcgtaa ggggcacaat taattgtcca ttcacagaag gaggacaaag gaaggggacg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NheI(407) ..........

361 tattctgctc aaccttccta tcagaaactg cagtatctgt atttttgcta gcagtaatac 420

361 ataagacgag ttggaaggat agtctttgac gtcatagaca taaaaacgat cgtcattatg 420

pGENE_fwd_pri ...... .......... .......... .......... ..........

.......... .......... ......... HindIII(453) KpnI(463) BamHI(471)

421 taacggttct ttttttctct tcacaggcca ccaagcttgg taccgagctc ggatccacta 480

421 attgccaaga aaaaaagaga agtgtccggt ggttcgaacc atggctcgag cctaggtgat 480

.......... .......... .......... .......... .......... ..........

.......... EcoRI(494) .......... .......... .......... ..........

481 gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 540

481 caggtcacac caccttaaga cgtctatagg tcgtgtcacc gccggcgagc tcagatctcc 540

.......... .......... .......... .......... .......... ..........

ApaI(543),BstBI(546) .......... .......... .......... AgeI(596)

541 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta 600

541 cgggcgccaa gcttccattc ggatagggat tgggagagga gccagagcta agatgcgcat 600

.......... .......... .......... V5 .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt 660

601 ggccagtagt agtggtagtg gtaactcaaa tttgggcgac tagtcggagc tgacacggaa 660

6xHis .......... .......... ........ BGH_rev_pri bGH_PA_term

.......... .......... .......... .......... .......... ..........

661 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 720

661 gatcaacggt cggtagacaa caaacgggga gggggcacgg aaggaactgg gaccttccac 720

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 780

721 ggtgagggtg acaggaaagg attattttac tcctttaacg tagcgtaaca gactcatcca 780

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 840

781 cagtaagata agacccccca ccccaccccg tcctgtcgtt ccccctccta acccttctgt 840

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 900

841 tatcgtccgt acgaccccta cgccacccga gataccgaag actccgcctt tcttggtcga 900

......... bGH_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 960

901 ccccgagatc ccccataggg gtgcgcggga catcgccgcg taattcgcgc cgcccacacc 960

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

961 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 1020

961 accaatgcgc gtcgcactgg cgatgtgaac ggtcgcggga tcgcgggcga ggaaagcgaa 1020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 1080

1021 agaagggaag gaaagagcgg tgcaagcggc cgaaaggggc agttcgagat ttagcccccg 1080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 1140

1081 agggaaatcc caaggctaaa tcacgaaatg ccgtggagct ggggtttttt gaactaatcc 1140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 1200

1141 cactaccaag tgcatcaccc ggtagcggga ctatctgcca aaaagcggga aactgcaacc 1200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 1260

1201 tcaggtgcaa gaaattatca cctgagaaca aggtttgacc ttgttgtgag ttgggataga 1260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 1320

1261 gccagataag aaaactaaat attccctaaa acggctaaag ccggataacc aattttttac 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 1380

1321 tcgactaaat tgtttttaaa ttgcgcttaa ttaagacacc ttacacacag tcaatcccac 1380

.......... .......... .......... ..... pBABE_3_pri SV40_enh .....

.......... .......... .......... .......... .......... ..........

1381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 1440

1381 acctttcagg ggtccgaggg gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag 1440

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 1500

1441 tcgttggtcc acacctttca ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt 1500

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc c cctaactcc 1560

1501 agagttaatc agtcgttggt atcagggcgg ggattgaggc gggtagggcg gggattgagg 1560

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

1561 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 1620

1561 cgggtcaagg cgggtaagag gcggggtacc gactgattaa aaaaaataaa tacgtctccg 1620

SV40_enh ..... SV40_pro SV40_origin ..... ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

1621 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 1680

1621 gctccggcgg agacggagac tcgataaggt cttcatcact cctccgaaaa aacctccgga 1680

.......... SV40_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt 1740

1681 tccgaaaacg tttttcgagg gccctcgaac atataggtaa aagcctagac tagtcgtgca 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac 1800

1741 caactgttaa ttagtagccg tatcatatag ccgtatcata ttatgctgtt ccactccttg 1800

.......... .......... EM7_pro .......... .......... ..........

MscI(1806) .......... .......... .......... .......... ..........

1801 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga 1860

1801 atttggtacc ggttcaactg gtcacggcaa ggccacgagt ggcgcgcgct gcagcggcct 1860

.......... .......... ......... bleo sh_ble ......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc 1920

1861 cgccagctca agacctggct ggccgagccc aagagggccc tgaagcacct cctgctgaag 1920

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg 1980

1921 cggccacacc aggccctgct gcactgggac aagtagtcgc gccaggtcct ggtccaccac 1980

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg 2040

1981 ggcctgttgt gggaccggac ccacacccac gcgccggacc tgctcgacat gcggctcacc 2040

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc 2100

2041 agcctccagc acaggtgctt gaaggccctg cggaggcccg gccggtactg gctctagccg 2100

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc 2160

2101 ctcgtcggca cccccgccct caagcgggac gcgctgggcc ggccgttgac gcacgtgaag 2160

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat 2220

2161 caccggctcc tcgtcctgac tgtgcacgat gctctaaagc taaggtggcg gcggaagata 2220

bleo sh_ble ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 2280

2221 ctttccaacc cgaagcctta gcaaaaggcc ctgcggccga cctactagga ggtcgcgccc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 2340

2281 ctagagtacg acctcaagaa gcgggtgggg ttgaacaaat aacgtcgaat attaccaatg 2340

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

2341 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2400

2341 tttatttcgt tatcgtagtg tttaaagtgt ttatttcgta aaaaaagtga cgtaagatca 2400

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacatctagc 2460

2401 acaccaaaca ggtttgagta gttacataga atagtacaga catatggcag ctgtagatcg 2460

EBV_rev_pri SV40_PA_term ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 2520

2461 atctcgaacc gcattagtac cagtatcgac aaaggacaca ctttaacaat aggcgagtgt 2520

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

2521 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 2580

2521 taaggtgtgt tgtatgctcg gccttcgtat ttcacatttc ggaccccacg gattactcac 2580

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 2640

2581 tcgattgagt gtaattaacg caacgcgagt gacgggcgaa aggtcagccc tttggacagc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 2700

2641 acggtcgacg taattactta gccggttgcg cgcccctctc cgccaaacgc ataacccgcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 2760

2701 agaaggcgaa ggagcgagtg actgagcgac gcgagccagc aagccgacgc cgctcgccat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 2820

2761 agtcgagtga gtttccgcca ttatgccaat aggtgtctta gtcccctatt gcgtcctttc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 2880

2821 ttgtacactc gttttccggt cgttttccgg tccttggcat ttttccggcg caacgaccgc 2880

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2881 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 2940

2881 aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt tagctgcgag ttcagtctcc 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 3000

2941 accgctttgg gctgtcctga tatttctatg gtccgcaaag ggggaccttc gagggagcac 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 3060

3001 gcgagaggac aaggctggga cggcgaatgg cctatggaca ggcggaaaga gggaagccct 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 3120

3061 tcgcaccgcg aaagagtatc gagtgcgaca tccatagagt caagccacat ccagcaagcg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 3180

3121 aggttcgacc cgacacacgt gcttgggggg caagtcgggc tggcgacgcg gaataggcca 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 3240

3181 ttgatagcag aactcaggtt gggccattct gtgctgaata gcggtgaccg tcgtcggtga 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 3300

3241 ccattgtcct aatcgtctcg ctccatacat ccgccacgat gtctcaagaa cttcaccacc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 3360

3301 ggattgatgc cgatgtgatc ttcttgtcat aaaccataga cgcgagacga cttcggtcaa 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 3420

3361 tggaagcctt tttctcaacc atcgagaact aggccgtttg tttggtggcg accatcgcca 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 3480

3421 aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 3540

3481 tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 3600

3541 tactctaata gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 3660

3601 agttagattt catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc 3660

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3661 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgcg 3720

3661 cgtggataga gtcgctagac agataaagca agtaggtatc aacggactga ggggcagcgc 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 3780

3721 atctattgat gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 3840

3781 ctgggtgcga gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 3900

3841 gcgtcttcac caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... FspI(3930) .......... .......... ..........

3901 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 3960

3901 cgatctcatt catcaagcgg tcaattatca aacgcgttgc aacaacggta acgatgtccg 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 4020

3961 tagcaccaca gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 4080

4021 tccgctcaat gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 4140

4081 tagcaacagt cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 4200

4141 ttaagagaat gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 4260

4201 ttcagtaaga ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttatgcc 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 4320

4261 ctattatggc gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 4380

4321 cccgcttttg agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 4440

4381 cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 4500

4441 ccttccgttt tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 4560

4501 gagaaggaaa aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg 4560

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4561 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 4620

4561 tataaactta cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtgccacctg acgtcgacgg atcgggagat cgta...... .......... .......... 4654

4621 cacggtggac tgcagctgcc tagccctcta gcat...... .......... .......... 4654

.......... .......... .......... .......... .......... ..........