f f!LOCUS pGene/V5-His C (GeneSwitch) 4646 bp DNA SYN 31-Oct-2007 DEFINITION pGene/V5-His C (GeneSwitch) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4646 /organism="pGene/V5-His C (GeneSwitch)" /mol_type="other DNA" misc_feature 190..214 /label="LNCX_primer" misc_feature 357..375 /label="pGENE_fwd_primer" misc_feature 547..588 /label="V5" misc_feature 598..615 /label="6xHis" misc_feature complement(638..655) /label="BGH_rev_primer" terminator 641..868 /label="bGH_PA_terminator" rep_origin 931..1237 /label="f1_origin" misc_feature complement(1351..1371) /label="pBABE_3_primer" misc_feature complement(1357..1572) /label="SV40_enhancer" promoter 1369..1637 /label="SV40_promoter" rep_origin 1536..1613 /label="SV40_origin" misc_feature 1598..1617 /label="SV40pro_F_primer" promoter 1731..1798 /label="EM7_promoter" gene 1799..2170 /label="bleo" /gene="bleo" misc_feature 1799..2173 /label="sh_ble" terminator 2306..2425 /label="SV40_PA_terminator" misc_feature 2394..2413 /label="EBV_rev_primer" misc_feature complement(2469..2487) /label="M13_reverse_primer" misc_feature complement(2486..2508) /label="M13_pUC_rev_primer" promoter complement(2522..2551) /label="lac_promoter" rep_origin complement(2860..3476) /label="pBR322_origin" CDS complement(3631..4491) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYATGSQATMDERNRQIA EIGASLIKHW*" gene complement(3631..4491) /label="Ampicillin" /gene="Ampicillin" promoter complement(4533..4561) /label="AmpR_promoter" ORIGIN 1 CCGAGCTCTT ACGCGGGTCG AAGCGGAGTA CTGTCCTCCG AGTGGAGTAC TGTCCTCCGA 61 GCGGAGTACT GTCCTCCGAG TCGAGGGTCG AAGCGGAGTA CTGTCCTCCG AGTGGAGTAC 121 TGTCCTCCGA GCGGAGTACT GTCCTCCGAG TCGACTCTAG AGGGTATATA ATGGATCTCG 181 AGATATCGGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT 241 TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGC GGCCGGGAAC GGTGCATTGG 301 AACGCGCATT CCCCGTGTTA ATTAACAGGT AAGTGTCTTC CTCCTGTTTC CTTCCCCTGC 361 TATTCTGCTC AACCTTCCTA TCAGAAACTG CAGTATCTGT ATTTTTGCTA GCAGTAATAC 421 TAACGGTTCT TTTTTTCTCT TCACAGGCCA CCAAGCTTGG TACCGAGCTC GGATCCACTA 481 GTCCAGTGTG GTGGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG AGGTCACCCA 541 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT 601 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 661 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 721 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 781 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 841 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG CTGGGGCTCT 901 AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 961 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 1021 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 1081 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 1141 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 1201 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 1261 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 1321 TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG TGTGGAAAGT 1381 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA 1441 GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 1501 AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT 1561 CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG 1621 CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT 1681 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA 1741 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT 1801 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA 1861 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT 1921 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA 1981 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT 2041 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC 2101 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA 2161 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT 2221 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT 2281 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG 2341 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT 2401 GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACATCTA GCTAGAGCTT 2461 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA 2521 CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT 2581 CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT 2641 GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 2701 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA 2761 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 2821 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 2881 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 2941 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 3001 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 3061 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 3121 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 3181 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 3241 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 3301 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 3361 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT 3421 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 3481 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 3541 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 3601 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 3661 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG CGTAGATAAC 3721 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 3781 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 3841 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 3901 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 3961 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 4021 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 4081 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 4141 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 4201 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 4261 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 4321 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 4381 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 4441 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 4501 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 4561 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 4621 TGACGTCGAC GGATCGGGAG ATCGTA // &pGene/V5-His C (GeneSwitch) "Times New Roman! fC&LNCX_pri"Times New Roman fC&ew pGENE_fwd_pri"Times New Roman fCɆ&#LV5"Times New Roman f&Vg6xHis"Times New Roman fC&~ BGH_rev_pri"Times New Roman fC&d bGH_PA_term"Times New Roman fCɆ& f1_origin"Times New Roman f&G[ pBABE_3_pri"Times New Roman fC&M$SV40_enh"Times New Roman fC&YeSV40_pro"Times New Roman* fCɆ&M SV40_origin"Times New Roman f&>Q SV40pro_F_pri"Times New Roman fC&EM7_pro"Times New Roman fC&zbleo"Times New Roman fCɆ&}sh_ble"Times New Roman( f& y  SV40_PA_term"Times New Roman fC&Z m  EBV_rev_pri"Times New Roman fC& M13_reverse_pri"Times New Roman fCɆ& M13_pUC_rev_pri"Times New Roman f& lac_pro"Times New Roman fC&,  pBR322_origin"Times New Roman fC&/ORF 1"Times New Roman fCɆ&/ Ampicillin"Times New Roman( f&AmpR_pro"Times New Roman fXbaI(156),SacII(280)"Times New Roman& "i fUNheI(407),HindIII(453),KpnI(463),BamHI(471),SpeI(477),EcoRI(494),NotI(521),BstBI(542)"Times New Roman&glqq1 fAgeI"Times New RomanP& fStuI(1669),MscI(1802)"Times New Roman&; fFspI"Times New RomanV&2*!! ftest"Times New Roman&CM??\ ftest"Times New Roman&~ ~& ftest"Times New Roman&~~ ftest"Times New Roman &bu