Sequence of pGene_V5-His_C

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Sequence of pGene_V5-His_C_(GeneSwitch)

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Sequence of pGene/V5-His C (GeneSwitch) : 4646 BP; 1052 A; 1238 C; 1186 G; 1170 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ccgagctctt acgcgggtcg aagcggagta ctgtcctccg agtggagtac tgtcctccga 60

1 ggctcgagaa tgcgcccagc ttcgcctcat gacaggaggc tcacctcatg acaggaggct 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcggagtact gtcctccgag tcgagggtcg aagcggagta ctgtcctccg agtggagtac 120

61 cgcctcatga caggaggctc agctcccagc ttcgcctcat gacaggaggc tcacctcatg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgtcctccga gcggagtact gtcctccgag tcgactctag agggtatata atggatctcg 180

121 acaggaggct cgcctcatga caggaggctc agctgagatc tcccatatat tacctagagc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agatatcgga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt 240

181 tctatagcct cgagcaaatc acttggcagt ctagcggacc tctgcggtag gtgcgacaaa 240

.......... .......... LNCX_pri .......... .......... ..........

.......... .......... .......... SacII(280) .......... ..........

241 tgacctccat agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg 300

241 actggaggta tcttctgtgg ccctggctag gtcggaggcg ccggcccttg ccacgtaacc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aacgcgcatt ccccgtgtta attaacaggt aagtgtcttc ctcctgtttc cttcccctgc 360

301 ttgcgcgtaa ggggcacaat taattgtcca ttcacagaag gaggacaaag gaaggggacg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tattctgctc aaccttccta tcagaaactg cagtatctgt atttttgcta gcagtaatac 420

361 ataagacgag ttggaaggat agtctttgac gtcatagaca taaaaacgat cgtcattatg 420

pGENE_fwd_pri ...... .......... .......... .......... ..........

.......... .......... ......... HindIII(453) KpnI(463) BamHI(471)

421 taacggttct ttttttctct tcacaggcca ccaagcttgg taccgagctc ggatccacta 480

421 attgccaaga aaaaaagaga agtgtccggt ggttcgaacc atggctcgag cctaggtgat 480

.......... .......... .......... .......... .......... ..........

.......... EcoRI(494) .......... .......... .......... ..........

481 gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg aggtcaccca 540

481 caggtcacac caccttaaga cgtctatagg tcgtgtcacc gccggcgagc tccagtgggt 540

.......... .......... .......... .......... .......... ..........

BstBI(542) .......... .......... .......... .......... AgeI(592)

541 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat 600

541 aagcttccat tcggataggg attgggagag gagccagagc taagatgcgc atggccagta 600

.......... .......... V5 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc 660

601 gtagtggtag tggtaactca aatttgggcg actagtcgga gctgacacgg aagatcaacg 660

6xHis .......... .......... ... BGH_rev_pri bGH_PA_term ...

.......... .......... .......... .......... .......... ..........

661 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 720

661 gtcggtagac aacaaacggg gagggggcac ggaaggaact gggaccttcc acggtgaggg 720

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 780

721 tgacaggaaa ggattatttt actcctttaa cgtagcgtaa cagactcatc cacagtaaga 780

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 840

781 taagaccccc caccccaccc cgtcctgtcg ttccccctcc taacccttct gttatcgtcc 840

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct 900

841 gtacgacccc tacgccaccc gagataccga agactccgcc tttcttggtc gaccccgaga 900

......... bGH_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 960

901 tcccccatag gggtgcgcgg gacatcgccg cgtaattcgc gccgcccaca ccaccaatgc 960

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

961 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 1020

961 gcgtcgcact ggcgatgtga acggtcgcgg gatcgcgggc gaggaaagcg aaagaaggga 1020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 1080

1021 aggaaagagc ggtgcaagcg gccgaaaggg gcagttcgag atttagcccc cgagggaaat 1080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 1140

1081 cccaaggcta aatcacgaaa tgccgtggag ctggggtttt ttgaactaat cccactacca 1140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 1200

1141 agtgcatcac ccggtagcgg gactatctgc caaaaagcgg gaaactgcaa cctcaggtgc 1200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 1260

1201 aagaaattat cacctgagaa caaggtttga ccttgttgtg agttgggata gagccagata 1260

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 1320

1261 agaaaactaa atattcccta aaacggctaa agccggataa ccaatttttt actcgactaa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 1380

1321 attgttttta aattgcgctt aattaagaca ccttacacac agtcaatccc acacctttca 1380

.......... .......... .......... ..... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

1381 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 1440

1381 ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt 1440

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 1500

1441 ccacaccttt caggggtccg aggggtcgtc cgtcttcata cgtttcgtac gtagagttaa 1500

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact c cgcccagtt 1560

1501 tcagtcgttg gtatcagggc ggggattgag gcgggtaggg cggggattga ggcgggtcaa 1560

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

1561 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 1620

1561 ggcgggtaag aggcggggta ccgactgatt aaaaaaaata aatacgtctc cggctccggc 1620

SV40_enh ..... SV40_pro SV40_origin ... SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

1621 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 1680

1621 ggagacggag actcgataag gtcttcatca ctcctccgaa aaaacctccg gatccgaaaa 1680

SV40_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa 1740

1681 cgtttttcga gggccctcga acatataggt aaaagcctag actagtcgtg cacaactgtt 1740

.......... .......... .......... .......... .......... EM7_pro

.......... .......... .......... .......... .......... ..........

1741 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat 1800

1741 aattagtagc cgtatcatat agccgtatca tattatgctg ttccactcct tgatttggta 1800

.......... .......... EM7_pro .......... .......... ..........

MscI(1802) .......... .......... .......... .......... ..........

1801 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga 1860

1801 ccggttcaac tggtcacggc aaggccacga gtggcgcgcg ctgcagcggc ctcgccagct 1860

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt 1920

1861 caagacctgg ctggccgagc ccaagagggc cctgaagcac ctcctgctga agcggccaca 1920

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa 1980

1921 ccaggccctg ctgcactggg acaagtagtc gcgccaggtc ctggtccacc acggcctgtt 1980

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt 2040

1981 gtgggaccgg acccacaccc acgcgccgga cctgctcgac atgcggctca ccagcctcca 2040

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc 2100

2041 gcacaggtgc ttgaaggccc tgcggaggcc cggccggtac tggctctagc cgctcgtcgg 2100

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga 2160

2101 cacccccgcc ctcaagcggg acgcgctggg ccggccgttg acgcacgtga agcaccggct 2160

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt 2220

2161 cctcgtcctg actgtgcacg atgctctaaa gctaaggtgg cggcggaaga tactttccaa 2220

bleo sh_ble ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 2280

2221 cccgaagcct tagcaaaagg ccctgcggcc gacctactag gaggtcgcgc ccctagagta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 2340

2281 cgacctcaag aagcgggtgg ggttgaacaa ataacgtcga atattaccaa tgtttatttc 2340

.......... .......... .......... ......... SV40_PA_term .........

.......... .......... .......... .......... .......... ..........

2341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 2400

2341 gttatcgtag tgtttaaagt gtttatttcg taaaaaaagt gacgtaagat caacaccaaa 2400

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacatcta gctagagctt 2460

2401 caggtttgag tagttacata gaatagtaca gacatatggc agctgtagat cgatctcgaa 2460

EBV_rev_pri SV40_PA_term ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 2520

2461 ccgcattagt accagtatcg acaaaggaca cactttaaca ataggcgagt gttaaggtgt 2520

....... M13_reverse_pri .... M13_pUC_rev_pri ....... ..........

.......... .......... .......... .......... .......... ..........

2521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 2580

2521 gttgtatgct cggccttcgt atttcacatt tcggacccca cggattactc actcgattga 2580

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 2640

2581 gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca gcacggtcga 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 2700

2641 cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg cgagaaggcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2760

2701 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2820

2761 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2880

2821 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 2880

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2940

2881 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3000

2941 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3060

3001 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3120

3061 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3180

3121 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3240

3181 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3300

3241 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3360

3301 gccgatgtga tcttcttgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 3420

3361 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caaaaaaaca 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 3480

3421 aacgttcgtc gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 3540

3481 atgccccaga ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtactctaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 3600

3541 tagtttttcc tagaagtgga tctaggaaaa tttaattttt acttcaaaat ttagttagat 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 3660

3601 ttcatatata ctcatttgaa ccagactgtc aatggttacg aattagtcac tccgtggata 3660

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3661 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg cgtagataac 3720

3661 gagtcgctag acagataaag caagtaggta tcaacggact gaggggcagc gcatctattg 3720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 3780

3721 atgctatgcc ctcccgaatg gtagaccggg gtcacgacgt tactatggcg ctctgggtgc 3780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 3840

3781 gagtggccga ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc 3840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 3900

3841 accaggacgt tgaaataggc ggaggtaggt cagataatta acaacggccc ttcgatctca 3900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... FspI(3926) .......... .......... ..........

3901 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 3960

3901 ttcatcaagc ggtcaattat caaacgcgtt gcaacaacgg taacgatgtc cgtagcacca 3960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 4020

3961 cagtgcgagc agcaaaccat accgaagtaa gtcgaggcca agggttgcta gttccgctca 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 4080

4021 atgtactagg gggtacaaca cgttttttcg ccaatcgagg aagccaggag gctagcaaca 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 4140

4081 gtcttcattc aaccggcgtc acaatagtga gtaccaatac cgtcgtgacg tattaagaga 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 4200

4141 atgacagtac ggtaggcatt ctacgaaaag acactgacca ctcatgagtt ggttcagtaa 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 4260

4201 gactcttatc acatacgccg ctggctcaac gagaacgggc cgcagttatg ccctattatg 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 4320

4261 gcgcggtgta tcgtcttgaa attttcacga gtagtaacct tttgcaagaa gccccgcttt 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 4380

4321 tgagagttcc tagaatggcg acaactctag gtcaagctac attgggtgag cacgtgggtt 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 4440

4381 gactagaagt cgtagaaaat gaaagtggtc gcaaagaccc actcgttttt gtccttccgt 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 4500

4441 tttacggcgt tttttccctt attcccgctg tgcctttaca acttatgagt atgagaagga 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 4560

4501 aaaagttata ataacttcgt aaatagtccc aataacagag tactcgccta tgtataaact 4560

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4561 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 4620

4561 tacataaatc tttttatttg tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgacgtcgac ggatcgggag atcgta.... .......... .......... .......... 4646

4621 actgcagctg cctagccctc tagcat.... .......... .......... .......... 4646

.......... .......... .......... .......... .......... ..........