{G{GC)LOCUS pGlow TOPO 5333 bp DNA SYN 27-Aug-2007 DEFINITION pGlow TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5333 /organism="pGlow TOPO" /mol_type="other DNA" promoter 17..35 /label="T7_promoter" misc_feature complement(173..201) /label="GFP_R_primer" misc_feature complement(217..236) /label="GFPfusionREV_primer" CDS 140..859 /label="GFP_ORF" /gene="GFP_ORF" /note="ORF frame 2" /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITHGMDELYK*" gene 140..859 /label="GFP_ORF" /gene="GFP_ORF" misc_feature 796..816 /label="GFP_F_primer" misc_feature complement(923..940) /label="BGH_rev_primer" terminator 926..1153 /label="bGH_PA_terminator" rep_origin 1216..1522 /label="f1_origin" misc_feature complement(1636..1656) /label="pBABE_3_primer" misc_feature complement(1642..1858) /label="SV40_enhancer" promoter 1654..1923 /label="SV40_promoter" rep_origin 1822..1899 /label="SV40_origin" misc_feature 1884..1903 /label="SV40pro_F_primer" CDS 2038..2832 /label="Neomycin" /gene="Neomycin" /note="ORF frame 1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 2041..2829 /label="Neomycin" /gene="Neomycin" terminator 3009..3128 /label="SV40_PA_terminator" misc_feature 3097..3116 /label="EBV_rev_primer" misc_feature complement(3172..3190) /label="M13_reverse_primer" misc_feature complement(3189..3211) /label="M13_pUC_rev_primer" promoter complement(3225..3254) /label="lac_promoter" rep_origin complement(3563..4182) /label="pBR322_origin" CDS complement(4337..5197) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4337..5197) /label="Ampicillin" /gene="Ampicillin" promoter complement(5239..5267) /label="AmpR_promoter" ORIGIN 1 GACGGATCGG GAGATCTAAT ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA 61 ACTTAAGCTT GGTACCGAGC TCGGATCCAC TAGTCCAGTG TGGTGGAATT GCCCTTAAGG 121 GCAATTCTGC AGATCTAGAA TGGCTAGCAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC 181 AATTCTTGTT GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG 241 TGAAGGTGAT GCTACATACG GAAAGCTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT 301 ACCTGTTCCA TGGCCAACAC TTGTCACTAC TTTCTCTTAT GGTGTTCAAT GCTTTTCCCG 361 TTATCCGGAT CATATGAAAC GGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGTTATGT 421 ACAGGAACGC ACTATATCTT TCAAAGATGA CGGGAACTAC AAGACGCGTG CTGAAGTCAA 481 GTTTGAAGGT GATACCCTTG TTAATCGTAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA 541 TGGAAACATT CTCGGACACA AACTCGAGTA CAACTATAAC TCACACAATG TATACATCAC 601 GGCAGACAAA CAAAAGAATG GAATCAAAGC TAACTTCAAA ATTCGCCACA ACATTGAAGA 661 TGGATCCGTT CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT 721 CCTTTTACCA GACAACCATT ACCTGTCGAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA 781 AAAGCGTGAC CACATGGTCC TTCTTGAGTT TGTAACTGCT GCTGGGATTA CACATGGCAT 841 GGATGAGCTC TACAAATAAT GAATTATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG 901 CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 961 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 1021 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 1081 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 1141 GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC 1201 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 1261 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 1321 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGCATCC CTTTAGGGTT CCGATTTAGT 1381 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 1441 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 1501 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 1561 GGGATTTTGG GGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 1621 GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 1681 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC 1741 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA 1801 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG 1861 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG 1921 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG 1981 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG 2041 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC 2101 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG 2161 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 2221 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC 2281 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT 2341 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG 2401 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC 2461 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 2521 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC 2581 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC 2641 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA 2701 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC 2761 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 2821 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC 2881 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT 2941 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC 3001 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 3061 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 3121 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT 3181 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA 3241 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC 3301 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC 3361 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT 3421 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 3481 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 3541 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 3601 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 3661 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 3721 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC 3781 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 3841 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 3901 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 3961 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 4021 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 4081 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4141 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 4201 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4261 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 4321 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 4381 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 4441 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 4501 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 4561 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 4621 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 4681 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 4741 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 4801 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 4861 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 4921 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 4981 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 5041 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 5101 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 5161 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 5221 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 5281 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTC // pGlow TOPO "Times New Roman"{GC#T7_pro"Times New Roman{G[ORF 2"Times New Roman{GC[GFP_ORF"Times New Roman{GC GFP_R_pri"Times New Roman*{GCɆGFPfusionREV_pri"Times New Roman*{GC0 GFP_F_pri"Times New Roman*{GCɆ BGH_rev_pri"Times New Roman{G bGH_PA_term"Times New Roman{GC f1_origin"Times New Roman{GCdx pBABE_3_pri"Times New Roman{GCɆjBSV40_enh"Times New Roman{GvSV40_pro"Times New Roman*{GCk SV40_origin"Times New Roman{GC\o SV40pro_F_pri"Times New Roman{GCɆ ORF 1"Times New Roman{G Neomycin"Times New Roman{GC 8  SV40_PA_term"Times New Roman{GC ,  EBV_rev_pri"Times New Roman{GCɆd v M13_reverse_pri"Times New Roman{Gu M13_pUC_rev_pri"Times New Roman{GC lac_pro"Times New Roman{GC V pBR322_origin"Times New Roman{GCɆMORF 2"Times New Roman{GM Ampicillin"Times New Roman{GCwAmpR_pro"Times New Roman {GKpnI(75),SpeI(89)"Times New RomanK{q^@^ {GNdeI"Times New Romant}n%} {GNotI(880),ApaI(902)"Times New Romanpdltt {GStuI(1955),SmaI(1979)"Times New RomanIQXX% {GNarI"Times New Romanv!!*&2&2cA {GAatII"Times New Roman**{nU}FU {Gtest"Times New Roman;IVV9 {Gtest"Times New Romand_(( {Gtest"Times New Roman; %$5ErEz