Sequence of pGlow

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pGlow_TOPO

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pGlow TOPO : 5333 BP; 1277 A; 1362 C; 1338 G; 1356 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctaat acgactcact atagggagac ccaagctggc tagcgtttaa 60

1 ctgcctagcc ctctagatta tgctgagtga tatccctctg ggttcgaccg atcgcaaatt 60

.......... .......... T7_pro .......... .......... ..........

.......... KpnI(75) SpeI(89) .......... .......... ..........

61 acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt gcccttaagg 120

61 tgaattcgaa ccatggctcg agcctaggtg atcaggtcac accaccttaa cgggaattcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcaattctgc agatctagaa tggctagcaa aggagaagaa cttttcactg gagttgtccc 180

121 cgttaagacg tctagatctt accgatcgtt tcctcttctt gaaaagtgac ctcaacaggg 180

.......... .......... ........ ORF 2 GFP_ORF ........ ..........

.......... .......... .......... .......... .......... ..........

181 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 240

181 ttaagaacaa cttaatctac cactacaatt acccgtgttt aaaagacagt cacctctccc 240

........ GFP_R_pri ORF 2 GFP_ORF ..... GFPfusionREV_pri .......

.......... .......... .......... .......... .......... ..........

241 tgaaggtgat gctacatacg gaaagcttac ccttaaattt atttgcacta ctggaaaact 300

241 acttccacta cgatgtatgc ctttcgaatg ggaatttaaa taaacgtgat gaccttttga 300

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

301 acctgttcca tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcccg 360

301 tggacaaggt accggttgtg aacagtgatg aaagagaata ccacaagtta cgaaaagggc 360

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... NdeI(372) .......... .......... .......... ..........

361 ttatccggat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt 420

361 aataggccta gtatactttg ccgtactgaa aaagttctca cggtacgggc ttccaataca 420

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 acaggaacgc actatatctt tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa 480

421 tgtccttgcg tgatatagaa agtttctact gcccttgatg ttctgcgcac gacttcagtt 480

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtttgaaggt gatacccttg ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga 540

481 caaacttcca ctatgggaac aattagcata gctcaatttt ccataactaa aatttcttct 540

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 tggaaacatt ctcggacaca aactcgagta caactataac tcacacaatg tatacatcac 600

541 acctttgtaa gagcctgtgt ttgagctcat gttgatattg agtgtgttac atatgtagtg 600

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca acattgaaga 660

601 ccgtctgttt gttttcttac cttagtttcg attgaagttt taagcggtgt tgtaacttct 660

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 tggatccgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 720

661 acctaggcaa gttgatcgtc tggtaatagt tgttttatga ggttaaccgc taccgggaca 720

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccttttacca gacaaccatt acctgtcgac acaatctgcc ctttcgaaag atcccaacga 780

721 ggaaaatggt ctgttggtaa tggacagctg tgttagacgg gaaagctttc tagggttgct 780

.......... ........ ORF 2 GFP_ORF ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat 840

781 tttcgcactg gtgtaccagg aagaactcaa acattgacga cgaccctaat gtgtaccgta 840

.......... ... ORF 2 GFP_ORF GFP_F_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggatgagctc tacaaataat gaattatcca gcacagtggc ggccgctcga gtctagaggg 900

841 cctactcgag atgtttatta cttaataggt cgtgtcaccg ccggcgagct cagatctccc 900

ORF 2 GFP_ORF ...... .......... .......... .......... ..........

ApaI(902) .......... .......... .......... .......... ..........

901 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 960

901 gggcaaattt gggcgactag tcggagctga cacggaagat caacggtcgg tagacaacaa 960

.......... .......... ........ BGH_rev_pri bGH_PA_term ........

.......... .......... .......... .......... .......... ..........

961 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 1020

961 acggggaggg ggcacggaag gaactgggac cttccacggt gagggtgaca ggaaaggatt 1020

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 1080

1021 attttactcc tttaacgtag cgtaacagac tcatccacag taagataaga ccccccaccc 1080

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 1140

1081 caccccgtcc tgtcgttccc cctcctaacc cttctgttat cgtccgtacg acccctacgc 1140

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 1200

1141 cacccgagat accgaagact ccgcctttct tggtcgaccc cgagatcccc cataggggtg 1200

bGH_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 1260

1201 cgcgggacat cgccgcgtaa ttcgcgccgc ccacaccacc aatgcgcgtc gcactggcga 1260

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1261 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 1320

1261 tgtgaacggt cgcgggatcg cgggcgagga aagcgaaaga agggaaggaa agagcggtgc 1320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt ccgatttagt 1380

1321 aagcggccga aaggggcagt tcgagattta gccccgtagg gaaatcccaa ggctaaatca 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 1440

1381 cgaaatgccg tggagctggg gttttttgaa ctaatcccac taccaagtgc atcacccggt 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 1500

1441 agcgggacta tctgccaaaa agcgggaaac tgcaacctca ggtgcaagaa attatcacct 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 1560

1501 gagaacaagg tttgaccttg ttgtgagttg ggatagagcc agataagaaa actaaatatt 1560

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 1620

1561 ccctaaaacc cctaaagccg gataaccaat tttttactcg actaaattgt ttttaaattg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 1680

1621 cgcttaatta agacacctta cacacagtca atcccacacc tttcaggggt ccgaggggtc 1680

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

1681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 1740

1681 cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtccac acctttcagg 1740

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 1800

1741 ggtccgaggg gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtat 1800

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 1860

1801 cagggcgggg attgaggcgg gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc 1860

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

1861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 1920

1861 ggggtaccga ctgattaaaa aaaataaata cgtctccggc tccggcggag acggagactc 1920

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(1955) .......... SmaI(1979)

1921 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 1980

1921 gataaggtct tcatcactcc tccgaaaaaa cctccggatc cgaaaacgtt tttcgagggc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 2040

1981 cctcgaacat ataggtaaaa gcctagacta gttctctgtc ctactcctag caaagcgtac 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 2100

2041 taacttgttc tacctaacgt gcgtccaaga ggccggcgaa cccacctctc cgataagccg 2100

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 2160

2101 atactgaccc gtgttgtctg ttagccgacg agactacggc ggcacaaggc cgacagtcgc 2160

.......... ........ ORF 1 Neomycin ........ .......... ..........

NarI(2166) .......... .......... .......... .......... ..........

2161 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 2220

2161 gtccccgcgg gccaagaaaa acagttctgg ctggacaggc cacgggactt acttgacgtc 2220

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 2280

2221 ctgctccgtc gcgccgatag caccgaccgg tgctgcccgc aaggaacgcg tcgacacgag 2280

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 2340

2281 ctgcaacagt gacttcgccc ttccctgacc gacgataacc cgcttcacgg ccccgtccta 2340

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 2400

2341 gaggacagta gagtggaacg aggacggctc tttcataggt agtaccgact acgttacgcc 2400

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 2460

2401 gccgacgtat gcgaactagg ccgatggacg ggtaagctgg tggttcgctt tgtagcgtag 2460

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 2520

2461 ctcgctcgtg catgagccta ccttcggcca gaacagctag tcctactaga cctgcttctc 2520

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 2580

2521 gtagtccccg agcgcggtcg gcttgacaag cggtccgagt tccgcgcgta cgggctgccg 2580

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 2640

2581 ctcctagagc agcactgggt accgctacgg acgaacggct tatagtacca ccttttaccg 2640

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 2700

2641 gcgaaaagac ctaagtagct gacaccggcc gacccacacc gcctggcgat agtcctgtat 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 2760

2701 cgcaaccgat gggcactata acgacttctc gaaccgccgc ttacccgact ggcgaaggag 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 2820

2761 cacgaaatgc catagcggcg agggctaagc gtcgcgtagc ggaagatagc ggaagaactg 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 2880

2821 ctcaagaaga ctcgccctga gaccccaagc tttactggct ggttcgctgc gggttggacg 2880

ORF 1 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 2940

2881 gtagtgctct aaagctaagg tggcggcgga agatactttc caacccgaag ccttagcaaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 3000

2941 aggccctgcg gccgacctac taggaggtcg cgcccctaga gtacgacctc aagaagcggg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3060

3001 tggggttgaa caaataacgt cgaatattac caatgtttat ttcgttatcg tagtgtttaa 3060

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa c tcatcaatg 3120

3061 agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt gagtagttac 3120

.......... ......... SV40_PA_term ........ EBV_rev_pri .........

.......... .......... .......... .......... .......... ..........

3121 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 3180

3121 atagaatagt acagacatat ggcagctgga gatcgatctc gaaccgcatt agtaccagta 3180

.......... .......... .......... .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

3181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 3240

3181 tcgacaaagg acacacttta acaataggcg agtgttaagg tgtgttgtat gctcggcctt 3240

M13_reverse_pri M13_pUC_rev_pri ........ .......... lac_pro

.......... .......... .......... .......... .......... ..........

3241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 3300

3241 cgtatttcac atttcggacc ccacggatta ctcactcgat tgagtgtaat taacgcaacg 3300

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 3360

3301 cgagtgacgg gcgaaaggtc agccctttgg acagcacggt cgacgtaatt acttagccgg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 3420

3361 ttgcgcgccc ctctccgcca aacgcataac ccgcgagaag gcgaaggagc gagtgactga 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3480

3421 gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc cgccattatg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3540

3481 ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3600

3541 tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag gcggggggac 3600

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3660

3601 tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt cctgatattt 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3720

3661 ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc tgggacggcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 3780

3721 aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga gttacgagtg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 3840

3781 cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca cacgtgcttg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 3900

3841 gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc aggttgggcc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 3960

3901 attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 4020

3961 tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg tgatcttcct 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4080

4021 gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4140

4081 gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4200

4141 aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4260

4201 gagtcacctt gcttttgagt gcaattccct aaaaccagta ctctaatagt ttttcctaga 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4320

4261 agtggatcta ggaaaattta atttttactt caaaatttag ttagatttca tatatactca 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 4380

4321 tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt cgctagacag 4380

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4381 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 4440

4381 ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc tatgccctcc 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 4500

4441 cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt ggccgaggtc 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 4560

4501 taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 4620

4561 ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca tcaagcggtc 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 4680

4621 aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt gcgagcagca 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 4740

4681 aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt actagggggt 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4800

4741 acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct tcattcaacc 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 4860

4801 ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga cagtacggta 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 4920

4861 ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact cttatcacat 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 4980

4921 acgccgctgg ctcaacgaga acgggccgca gttatgccct attatggcgc ggtgtatcgt 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5040

4981 cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag agttcctaga 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5100

5041 atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact agaagtcgta 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5160

5101 gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta cggcgttttt 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 5220

5161 tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa gttataataa 5220

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5280

5221 cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca taaatctttt 5280

.......... .......... .......... AmpR_pro .......... ..........

......... AatII(5300) ......... .......... .......... ..........

5281 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc....... 5333

5281 tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg cag....... 5333

.......... .......... .......... .......... .......... ..........