Sequence of pHIS-2

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Sequence of pHIS-2

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Sequence of pHIS-2 : 7207 BP; 1895 A; 1666 C; 1849 G; 1796 T; 1 other;

EcoRI(1) SmaI(8) SacI(16) .... SacII(39) .......... ..........

1 gaattcccgg ggagctcacg cgttcgcgaa tcgatccgcg gtctagaaat tcctggcatt 60

1 cttaagggcc cctcgagtgc gcaagcgctt agctaggcgc cagatcttta aggaccgtaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atcacataat gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa 120

61 tagtgtatta cttaatatgt aatatatttc attacactaa agaagcttct tatatgattt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaatgagcag gcaagataaa cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg 180

121 tttactcgtc cgttctattt gcttccgttt ctactgtctc gtctttcggg atcatttcgc 180

.......... .......... .......... .......... HIS3 ORF 2 ..........

.......... .......... .......... .......... .......... ..........

181 tattacaaat gaaaccaaga ttcagattgc gatctcttta aagggtggtc ccctagcgat 240

181 ataatgttta ctttggttct aagtctaacg ctagagaaat ttcccaccag gggatcgcta 240

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agagcactcg atcttcccag aaaaagaggc agaagcagta gcagaacagg ccacacaatc 300

241 tctcgtgagc tagaagggtc tttttctccg tcttcgtcat cgtcttgtcc ggtgtgttag 300

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcaagtgatt aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc 360

301 cgttcactaa ttgcaggtgt gtccatatcc caaagacctg gtatactatg tacgagaccg 360

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caagcattcc ggctggtcgc taatcgttga gtgcattggt gacttacaca tagacgacca 420

361 gttcgtaagg ccgaccagcg attagcaact cacgtaacca ctgaatgtgt atctgctggt 420

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcacaccact gaagactgcg ggattgctct cggtcaagct tttaaagagg ccctaggggc 480

421 agtgtggtga cttctgacgc cctaacgaga gccagttcga aaatttctcc gggatccccg 480

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgtgcgtgga gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg cactttccag 540

481 gcacgcacct catttttcca aacctagtcc taaacgcgga aacctactcc gtgaaaggtc 540

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agcggtggta gatctttcga acaggccgta cgcagttgtc gaacttggtt tgcaaaggga 600

541 tcgccaccat ctagaaagct tgtccggcat gcgtcaacag cttgaaccaa acgtttccct 600

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gaaagtagga gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc 660

601 ctttcatcct ctagagagaa cgctctacta gggcgtaaaa gaactttcga aacgtctccg 660

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tagcagaatt accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga 720

661 atcgtcttaa tgggaggtgc aactaacaga cgctccgttc ttactagtag tggcatcact 720

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... KpnI(777)

721 gagtgcgttc aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa 780

721 ctcacgcaag ttccgagaac gccaacggta ttctcttcgg tggagcgggt taccatggtt 780

.......... .......... HIS3 ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgatgttccc tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgcagc 840

781 gctacaaggg aggtggtttc cacaagaata catcactgtg gctaataaat ttcgacgtcg 840

.......... HIS3 ORF 2 .......... ........ HIS3_3UTR_term ........

.......... .......... .......... .......... .......... ..........

841 atacgatata tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg 900

841 tatgctatat atatgtacac atatatacat atggatactt acagtcattc atacatatgc 900

.......... ........ HIS3_3UTR_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 aacagtatga tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg 960

901 ttgtcatact atgacttcta ctgttccatt acgtagtaag atatgcacag taagacttgc 960

.......... ........ HIS3_3UTR_term ........ .......... ..........

.......... .......... .......... .......... .......... XhoI(1011)

961 aggcgcgctt tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgagaaaa 1020

961 tccgcgcgaa aggaaaaaag aaaaacgaaa aagaaaaaaa aagagaactt gagctctttt 1020

.......... ........ HIS3_3UTR_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaaatataaa agagatggag gaacgggaaa aagttagttg tggtgatagg tggcaagtgg 1080

1021 ttttatattt tctctacctc cttgcccttt ttcaatcaac accactatcc accgttcacc 1080

.......... ........ HIS3_3UTR_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tattccgtaa gaacaacaag aaaagcattt catattatgg ctgaactgag c gaacaagtg 1140

1081 ataaggcatt cttgttgttc ttttcgtaaa gtataatacc gacttgactc gcttgttcac 1140

.......... ........ HIS3_3UTR_term ........ ORF 1 ..........

.......... .......... .......... .......... ......... AflII(1191)

1141 caaaatttaa gcatcaacga caacaacgag aatggttatg ttcctcctca cttaagagga 1200

1141 gttttaaatt cgtagttgct gttgttgctc ttaccaatac aaggaggagt gaattctcct 1200

.......... ..... HIS3_3UTR_term ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaaccaagaa gtgccagaaa taacatgagc aactacaata acaacaacgg cggctacaac 1260

1201 tttggttctt cacggtcttt attgtactcg ttgatgttat tgttgttgcc gccgatgttg 1260

.......... ..... HIS3_3UTR_term ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggtggccgtg gcggtggcag cttctttagc aacaaccgtc gtggtggtta cggcaacggt 1320

1261 ccaccggcac cgccaccgtc gaagaaatcg ttgttggcag caccaccaat gccgttgcca 1320

.......... ..... HIS3_3UTR_term ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggtttcttcg gtggaaacaa cggtggcagc agatctaacg gccgttctgg tggtagatgg 1380

1321 ccaaagaagc cacctttgtt gccaccgtcg tctagattgc cggcaagacc accatctacc 1380

.......... ..... HIS3_3UTR_term ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcgatggca aacatgtccc agctccaaga aacgaaaagg ccgagatcgc catatttggt 1440

1381 tagctaccgt ttgtacaggg tcgaggttct ttgcttttcc ggctctagcg gtataaacca 1440

.......... ..... HIS3_3UTR_term ORF 1 ORF 2 ..... ..........

BamHI(1449) ........ .......... .......... .......... ..........

1441 gtccccgagg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc 1500

1441 caggggctcc taggagatgc ggcctgcgta gcaccggccg tagtggccgc ggtgtccacg 1500

HIS3_3UTR_term ... ORF 1 ORF 2 tet(1191-300) ....... ..........

.......... .......... .......... .......... .......... ..........

1501 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg 1560

1501 ccaacgaccg cggatatagc ggctgtagtg gctacccctt ctagcccgag cggtgaagcc 1560

..... ORF 1 ORF 2 tet(1191-300) pBRrevBam_pri ..... ..........

.......... .......... .......... .......... .......... ..........

1561 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt 1620

1561 cgagtactcg cgaacaaagc cgcacccata ccaccgtccg gggcaccggc cccctgacaa 1620

.......... .. ORF 1 ORF 2 tet(1191-300) .. .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct 1680

1621 cccgcggtag aggaacgtac gtggtaagga acgccgccgc cacgagttgc cggagttgga 1680

.......... .. ORF 1 ORF 2 tet(1191-300) .. .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac cgatgccctt 1740

1681 tgatgacccg acgaaggatt acgtcctcag cgtattccct ctcgcagctg gctacgggaa 1740

..... ORF 1 ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 1800

1741 ctctcggaag ttgggtcagt cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg 1800

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 1860

1801 tgaatactga cagaagaaat agtacgttga gcatcctgtc cacggccgtc gcgagaccca 1860

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 1920

1861 gtaaaagccg ctcctggcga aagcgacctc gcgctgctac tagccggaca gcgaacgcca 1920

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 1980

1921 taagccttag aacgtgcggg agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa 1980

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 2040

1981 gccgctcttc gtccggtaat agcggccgta ccgccggctg cgcgacccga tgcagaacga 2040

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 2100

2041 ccgcaagcgc tgcgctccga cctaccggaa ggggtaatac taagaagagc gaaggccgcc 2100

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 2160

2101 gtagccctac gggcgcaacg tccggtacga caggtccgtc catctactgc tggtagtccc 2160

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat 2220

2161 tgtcgaagtt cctagcgagc gccgagaatg gtcggattga agctagtaac ctggcgacta 2220

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 2280

2221 gcagtgccgc taaatacggc ggagccgctc gtgtaccttg cccaaccgta cctaacatcc 2280

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 2340

2281 gcggcgggat atggaacaga cggaggggcg caacgcagcg ccacgtacct cggcccggtg 2340

.......... ..... ORF 2 tet(1191-300) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga 2400

2341 gagctggact taccttcggc cgccgtggag cgattgccta agtggtgagg ttcttaacct 2400

ORF 2 tet(1191-300) .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc 2460

2401 cggttagtta agaacgcctc ttgacactta cgcgtttggt tgggaaccgt cttgtatagg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atcgcgtccg ccatctccag cagccgcacg cggcgcatcg gggggggggt ttcaattcaa 2520

2461 tagcgcaggc ggtagaggtc gtcggcgtgc gccgcgtagc ccccccccca aagttaagtt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttcatcattt tttttttatt cttttttttg atttcggttt ctttgaaatt tttttaacga 2580

2521 aagtagtaaa aaaaaaataa gaaaaaaaac taaagccaaa gaaactttaa aaaaattgct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cattactata tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac 2640

2581 gtaatgatat atatattata tccttcgtaa attatcttgt cgtagcatta tatacacatg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg 2700

2641 aaacgtcaat actgcggtct accgtcatca ccttctataa gaaataactt tttatcgaac 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc 2760

2701 agtggaatgc atgttagaac taggcctcga aaagaaaaaa acggctaatt cttaattaag 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg 2820

2761 ccagcttttt tcttttcctc tcccggttct ccctcccgta accactgata actcgtgcac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg 2880

2821 tcatatgcac taattcgtgt gtttccgtcg aacctcatac agacaataat taaagtgtcc 2880

.......... .......... .......... .......... ORF 2 TRP1 ..........

.......... .......... .......... .......... .......... ..........

2881 tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc 2940

2881 atcaagacca ggtaaccact ttcaaacgcc gaacgtctcg tgtctccggc gtcttacacg 2940

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat 3000

2941 agatctaagg ctacgactga acgacccata atatacacac gggttatctt tctcttgtta 3000

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg 3060

3001 actgggccaa taacgttcct tttaaagttc agaacatttt cgtatatttt tatcaagtcc 3060

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct 3120

3061 gtgaggcttt atgaaccaac cgcacaaagc attagttgga ttcctcctac aaaaccgaga 3120

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... ......... EcoRV(3142) ......... .......... ..........

3121 ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata 3180

3121 ccagttacta atgccgtaac tatagcaggt tgacgtacct ctactcagca ccgttcttat 3180

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat 3240

3181 ggttctcaag gagccaaacg gtcaataatt ttctgagcat aaaggttttc tgacgttgta 3240

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc 3300

3241 tgatgagtca cgtcgaagtg tctttggagt aagcaaataa gggaacaaac taagtcttcg 3300

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga 3360

3301 tccaccctgt ccacttgaaa acctaacctt gagctaaaga ctgacccaac cttccgttct 3360

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga 3420

3361 ctcggggctt tcgaatgtaa aatacaatcg accacctgac tgcggtcttt tacaaccact 3420

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg 3480

3421 acgcgaatct aatttaccgc aataaccaca actacattcg cctccacacc tctgtttacc 3480

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac 3540

3481 acattttctg agattgtttt atcgtttaaa gcagttttta cgattcttta tccaataatg 3540

.......... .......... ORF 2 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgagtagtat ttatttaagt attgtttgtg cacttgccct agagtcgacc tgcaggcatg 3600

3541 actcatcata aataaattca taacaaacac gtgaacggga tctcagctgg acgtccgtac 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc 3660

3601 gttcgaaaac aagggaaatc actcccaatt aaagctcgaa ccgcattagt accagtatcg 3660

.......... .......... T3_pro .......... ....... M13_reverse_pri

.......... .......... .......... .......... .......... ..........

3661 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 3720

3661 acaaaggaca cactttaaca ataggcgagt gttaaggtgt gttgtatgct cggccttcgt 3720

....... M13_pUC_rev_pri ....... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

3721 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 3780

3721 atttcacatt tcggacccca cggattactc actcgattga gtgtaattaa cgcaacgcga 3780

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 3840

3781 gtgacgggcg aaaggtcagc cctttggaca gcacggtcga cgtaattact tagccggttg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 3900

3841 cgcgcccctc tccgccaaac gcataacccg cgagaaggcg aaggagcgag tgactgagcg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 3960

3901 acgcgagcca gcaagccgac gccgctcgcc atagtcgagt gagtttccgc cattatgcca 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 4020

3961 ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 4080

4021 ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc 4080

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4081 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 4140

4081 tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt gggctgtcct gatatttcta 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 4200

4141 tggtccgcaa agggggacct tcgagggagc acgcgagagg acaaggctgg gacggcgaat 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 4260

4201 ggcctatgga caggcggaaa gagggaagcc cttcgcaccg cgaaagagta tcgagtgcga 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 4320

4261 catccataga gtcaagccac atccagcaag cgaggttcga cccgacacac gtgcttgggg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 4380

4321 ggcaagtcgg gctggcgacg cggaataggc cattgatagc agaactcagg ttgggccatt 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 4440

4381 ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 4500

4441 atccgccacg atgtctcaag aacttcacca ccggattgat gccgatgtga tcttcctgtc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 4560

4501 ataaaccata gacgcgagac gacttcggtc aatggaagcc tttttctcaa ccatcgagaa 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 4620

4561 ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc gtcgtctaat 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 4680

4621 gcgcgtcttt ttttcctaga gttcttctag gaaactagaa aagatgcccc agactgcgag 4680

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 4740

4681 tcaccttgct tttgagtgca attccctaaa accagtactc taatagtttt tcctagaagt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 4800

4741 ggatctagga aaatttaatt tttacttcaa aatttagtta gatttcatat atactcattt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cttggtctga cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat 4860

4801 gaaccagact gtcagtcttc ttgagcagtt cttccgctat cttccgctac gcgacgctta 4860

.......... .......... ... ORF 2 Neomycin(variant) ... ..........

.......... .......... .......... .......... .......... ..........

4861 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt 4920

4861 gccctcgccg ctatggcatt tcgtgctcct tcgccagtcg ggtaagcggc ggttcgagaa 4920

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc 4980

4921 gtcgttatag tgcccatcgg ttgcgataca ggactatcgc caggcggtgt gggtcggccg 4980

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat 5040

4981 gtgtcagcta cttaggtctt ttcgccggta aaaggtggta ctataagccg ttcgtccgta 5040

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgtcatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca 5100

5041 gcagtaccca gtgctgctct aggagcggca gcccgtacga gcggaactcg gaccgcttgt 5100

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg 5160

5101 caagccgacc gcgctcgggg actacgagaa gcaggtctag taggactagc tgttctggcc 5160

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg 5220

5161 gaaggtaggc tcatgcacga gcgagctacg ctacaaagcg aaccaccagc ttacccgtcc 5220

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg 5280

5221 atcggcctag ttcgcatacg tcggcggcgt aacgtagtcg gtactaccta tgaaagagcc 5280

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt 5340

5281 gtcctcgttc cactctactg tcctctagga cggggccgtg aagcgggtta tcgtcggtca 5340

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca 5400

5341 gggaagggcg aagtcactgt tgcagctcgt gtcgacgcgt tccttgcggg cagcaccggt 5400

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct 5460

5401 cggtgctatc ggcgcgacgg agcagaacgt caagtaagtc ccgtggcctg tccagccaga 5460

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc 5520

5461 actgtttttc ttggcccgcg gggacgcgac tgtcggcctt gtgccgccgt agtctcgtcg 5520

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac 5580

5521 gctaacagac aacacgggtc agtatcggct tatcggagag gtgggttcgc cggcctcttg 5580

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat tgaagcattt 5640

5581 gacgcacgtt aggtagaaca agttagtatg agaaggaaaa agttataata acttcgtaaa 5640

Neomycin(variant) ORF 2 .. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 5700

5641 tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt ttatttgttt 5700

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 taggggttcc gcgcacattt ccccgaaaag tgccacctgc ggacggatcg cttgcctgta 5760

5701 atccccaagg cgcgtgtaaa ggggcttttc acggtggacg cctgcctagc gaacggacat 5760

.......... .......... .......... .......... CEN6_ARS4 ..........

.......... .......... .......... .......... .......... ..........

5761 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta 5820

5761 tgaatgtgcg cggagcatag aaaattacta ccttattaaa cccttaaatg agacacaaat 5820

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagagtaga agagttacgg 5880

5821 aaataaaaat acaaaacata aacctaaaat ctttcattta tttctcatct tctcaatgcc 5880

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat 5940

5881 ttacttcttt ttttttattt gtttccaaat tttttaaagt tgtttttcgc atgaaatgta 5940

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta 6000

5941 tatataaata atctgttctt ttcgtctaat ttatctatat gtaagctaat tgctattcat 6000

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc 6060

6001 tttacatttt agtgtcctaa aagcacacac cagaagatgt gtctgttcta ctttgttaag 6060

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ggcattaata cctgagagca ggaagagcaa gataaaaggn agtatttgtt ggcgatcccc 6120

6061 ccgtaattat ggactctcgt ccttctcgtt ctattttccn tcataaacaa ccgctagggg 6120

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac 6180

6121 gatctcagaa aatgtagaag ccttttgttt ttgataaaaa agaaattaaa gaaaaaaatg 6180

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc 6240

6181 aaagataaaa attaaatata taaatataat tttttaaatt taatattaat aaaaatatcg 6240

.......... .......... CEN6_ARS4 .......... .......... ..........

.......... ......... AatII(6262) ......... .......... ..........

6241 acgtgatgaa aaggaccgac gtctaagaaa ccattattat catgacatta acctataaaa 6300

6241 tgcactactt ttcctggctg cagattcttt ggtaataata gtactgtaat tggatatttt 6300

CEN6_ARS4 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 6360

6301 tatccgcata gtgctccggg aaagcagagc gcgcaaagcc actactgcca cttttggaga 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 6420

6361 ctgtgtacgt cgagggcctc tgccagtgtc gaacagacat tcgcctacgg ccctcgtctg 6420

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 6480

6421 ttcgggcagt cccgcgcagt cgcccacaac cgcccacagc cccgaccgaa ttgatacgcc 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 6540

6481 gtagtctcgt ctaacatgac tctcacgtgg tatacgccac actttatggc gtgtctacgc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt 6600

6541 attcctcttt tatggcgtag tcctttaaca tttgcaatta taaaacaatt ttaagcgcaa 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 6660

6601 tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg ctttagccgt tttagggaat 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 6720

6661 atttagtttt cttatctggc tctatcccaa ctcacaacaa ggtcaaacct tgttctcagg 6720

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

6721 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 6780

6721 tgataatttc ttgcacctga ggttgcagtt tcccgctttt tggcagatag tcccgctacc 6780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 6840

6781 gggtgatgca cttggtagtg ggattagttc aaaaaacccc agctccacgg catttcgtga 6840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 6900

6841 tttagccttg ggatttccct cgggggctaa atctcgaact gcccctttcg gccgcttgca 6900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 6960

6901 ccgctctttc cttcccttct ttcgctttcc tcgcccgcga tcccgcgacc gttcacatcg 6960

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 7020

6961 ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta cgcggcgatg tcccgcgcag 7020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 7080

7021 cgcggtaagc ggtaagtccg acgcgttgac aacccttccc gctagccacg cccggagaag 7080

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 7140

7081 cgataatgcg gtcgaccgct ttccccctac acgacgttcc gctaattcaa cccattgcgg 7140

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact 7200

7141 tcccaaaagg gtcagtgctg caacattttg ctgccggtca cttaacatta tgctgagtga 7200

... M13_pUC_fwd_pri lacZ_a M13_forward20_pri ... ..........

.......... .......... .......... .......... .......... ..........

7201 atagggc... .......... .......... .......... .......... .......... 7207

7201 tatcccg... .......... .......... .......... .......... .......... 7207

.......... .......... .......... .......... .......... ..........