thLOCUS pHT3T7bm+ 3006 bp DNA SYN 11-Sep-2007 DEFINITION pHT3T7bm+ ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3006 /organism="pHT3T7bm+" /mol_type="other DNA" promoter complement(106..125) /label="T3_promoter" misc_feature complement(142..160) /label="M13_reverse_primer" misc_feature complement(159..181) /label="M13_pUC_rev_primer" promoter complement(195..224) /label="lac_promoter" rep_origin complement(533..1152) /label="pBR322_origin" CDS complement(1307..2167) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVREKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(1307..2167) /label="Ampicillin" /gene="Ampicillin" promoter complement(2209..2237) /label="AmpR_promoter" rep_origin 2315..2621 /label="f1_origin" misc_feature complement(2838..2986) /label="lacZ_a" misc_feature 2952..2974 /label="M13_pUC_fwd_primer" misc_feature 2967..2983 /label="M13_forward20_primer" promoter 2990..2 /label="T7_promoter" ORIGIN 1 gggcgaattc ccggggatcc acgcgtaact taagtgcggc cgcggtaccg ggcccgtcga 61 ctctagagct ctgcaggcat gcgatatccc atggaagctt ttgttccctt tagtgagggt 121 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 181 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 241 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 301 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 361 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 421 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 481 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 541 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 601 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 661 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 721 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg 781 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 841 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 901 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 961 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 1021 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 1081 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 1141 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 1201 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 1261 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 1321 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1381 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 1441 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 1501 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 1561 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 1621 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1681 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1741 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 1801 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctctg actggtgagt 1861 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 1921 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 1981 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 2041 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 2101 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 2161 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 2221 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 2281 cccgaaaagt gccacctacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 2341 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 2401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 2461 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga 2521 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 2581 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg 2641 ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt 2701 cgcctgctgg ggcaaaccac cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 2761 aagggcaatc agtatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 2821 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 2881 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 2941 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc 3001 actata // pHT3T7bm+ "Times New RomanC j}T3_pro"Times New RomanC T7_pro"Times New RomanC M13_reverse_pri"Times New RomanCɆ M13_pUC_rev_pri"Times New Roman lac_pro"Times New RomanC  pBR322_origin"Times New RomanC wORF 2"Times New RomanCɆ w Ampicillin"Times New Roman* AmpR_pro"Times New RomanC =  f1_origin"Times New RomanC  lacZ_a"Times New RomanCɆ M13_pUC_fwd_pri"Times New Roman M13_forward20_pri"Times New Roman ͖EcoRI(5),SmaI(12),BamHI(15),AflII(29),NotI(37),EagI(37),SacII(43),KpnI(48),ApaI(54),SalI(56),XbaI(62),SacI(70),PstI(75),EcoRV(85),NcoI(89),HindIII(95)"Times New Roman  H NarI"Times New Roman  b%b test"Times New Roman 8<NN test"Times New Romanb  p