Sequence of pHT3T7bm+

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Sequence of pHT3T7bm+

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Sequence of pHT3T7bm+ : 3006 BP; 712 A; 778 C; 755 G; 761 T; 0 other;

EcoRI SmaI,BamHI AflII NotI,EagI SacII3),KpnI(48)

1 gggcgaattc ccggggatcc acgcgtaact taagtgcggc cgcggtaccg ggcccgtcga 60

1 cccgcttaag ggcccctagg tgcgcattga attcacgccg gcgccatggc ccgggcagct 60

.......... .......... .......... .......... .......... ..........

XbaI SacI,PstI(75) EcoRV(85),NcoI(89) HindIII(95) ..

61 ctctagagct ctgcaggcat gcgatatccc atggaagctt ttgttccctt tagtgagggt 120

61 gagatctcga gacgtccgta cgctataggg taccttcgaa aacaagggaa atcactccca 120

.......... .......... .......... .......... .......... T3_pro

.......... .......... .......... .......... .......... ..........

121 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 180

121 attaaagctc gaaccgcatt agtaccagta tcgacaaagg acacacttta acaataggcg 180

.......... .......... .... M13_reverse_pri M13_pUC_rev_pri ....

.......... .......... .......... .......... .......... ..........

181 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 240

181 agtgttaagg tgtgttgtat gctcggcctt cgtatttcac atttcggacc ccacggatta 240

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 300

241 ctcactcgat tgagtgtaat taacgcaacg cgagtgacgg gcgaaaggtc agccctttgg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 360

301 acagcacggt cgacgtaatt acttagccgg ttgcgcgccc ctctccgcca aacgcataac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 420

361 ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag ccagcaagcc gacgccgctc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 480

421 gccatagtcg agtgagtttc cgccattatg ccaataggtg tcttagtccc ctattgcgtc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 540

481 ctttcttgta cactcgtttt ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 600

541 accgcaaaaa ggtatccgag gcggggggac tgctcgtagt gtttttagct gcgagttcag 600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 660

601 tctccaccgc tttgggctgt cctgatattt ctatggtccg caaaggggga ccttcgaggg 660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 720

661 agcacgcgag aggacaaggc tgggacggcg aatggcctat ggacaggcgg aaagagggaa 720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg 780

721 gcccttcgca ccgcgaaaga gttacgagtg cgacatccat agagtcaagc cacatccagc 780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 840

781 aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt cgggctggcg acgcggaata 840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 900

841 ggccattgat agcagaactc aggttgggcc attctgtgct gaatagcggt gaccgtcgtc 900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 960

901 ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc acgatgtctc aagaacttca 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 1020

961 ccaccggatt gatgccgatg tgatcttcct gtcataaacc atagacgcga gacgacttcg 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 1080

1021 gtcaatggaa gcctttttct caaccatcga gaactaggcc gtttgtttgg tggcgaccat 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 1140

1081 cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 1200

1141 taggaaacta gaaaagatgc cccagactgc gagtcacctt gcttttgagt gcaattccct 1200

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 1260

1201 aaaaccagta ctctaatagt ttttcctaga agtggatcta ggaaaattta atttttactt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 1320

1261 caaaatttag ttagatttca tatatactca tttgaaccag actgtcaatg gttacgaatt 1320

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

1321 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 1380

1321 agtcactccg tggatagagt cgctagacag ataaagcaag taggtatcaa cggactgagg 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 1440

1381 ggcagcacat ctattgatgc tatgccctcc cgaatggtag accggggtca cgacgttact 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 1500

1441 atggcgctct gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 1560

1501 cccggctcgc gtcttcacca ggacgttgaa ataggcggag gtaggtcaga taattaacaa 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 1620

1561 cggcccttcg atctcattca tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 1680

1621 gatgtccgta gcaccacagt gcgagcagca aaccataccg aagtaagtcg aggccaaggg 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 1740

1681 ttgctagttc cgctcaatgt actagggggt acaacacgtt ttttcgccaa tcgaggaagc 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 1800

1741 caggaggcta gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac caataccgtc 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctctg actggtgagt 1860

1801 gtgacgtatt aagagaatga cagtacggta ggcattctac gaaaagagac tgaccactca 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 1920

1861 tgagttggtt cagtaagact cttatcacat acgccgctgg ctcaacgaga acgggccgca 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 1980

1921 gttatgccct attatggcgc ggtgtatcgt cttgaaattt tcacgagtag taaccttttg 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 2040

1981 caagaagccc cgcttttgag agttcctaga atggcgacaa ctctaggtca agctacattg 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 2100

2041 ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 2160

2101 gtttttgtcc ttccgtttta cggcgttttt tcccttattc ccgctgtgcc tttacaactt 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 2220

2161 atgagtatga gaaggaaaaa gttataataa cttcgtaaat agtcccaata acagagtact 2220

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

2221 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 2280

2221 cgcctatgta taaacttaca taaatctttt tatttgttta tccccaaggc gcgtgtaaag 2280

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cccgaaaagt gccacctacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 2340

2281 gggcttttca cggtggatgc gcgggacatc gccgcgtaat tcgcgccgcc cacaccacca 2340

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

2341 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 2400

2341 atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc gggcgaggaa agcgaaagaa 2400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 2460

2401 gggaaggaaa gagcggtgca agcggccgaa aggggcagtt cgagatttag cccccgaggg 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga 2520

2461 aaatcccaag gctaaatcac gaaatgccgt ggagctgggg ttttttgaac taaacccact 2520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 2580

2521 accaagtgca tcacccggta gcgggactat ctgccaaaaa gcgggaaact gcaacctcag 2580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg 2640

2581 gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt tgtgagttgg gatagagccc 2640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt 2700

2641 gataagaaaa ctaaatattc cctaaaacgg ctaaagcctt ggtggtagtt tgtcctaaaa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgcctgctgg ggcaaaccac cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 2760

2701 gcggacgacc ccgtttggtg gcacctggcg aacgacgttg agagagtccc ggtccgccac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aagggcaatc agtatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 2820

2761 ttcccgttag tcatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta 2820

.......... .......... .......... .......... .......... ..........

NarI(2824) .......... .......... .......... .......... ..........

2821 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 2880

2821 gtccgcggta agcggtaagt ccgacgcgtt gacaaccctt cccgctagcc acgcccggag 2880

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 2940

2881 aagcgataat gcggtcgacc gctttccccc tacacgacgt tccgctaatt caacccattg 2940

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc 3000

2941 cggtcccaaa agggtcagtg ctgcaacatt ttgctgccgg tcacttaaca ttatgctgag 3000

.......... lacZ_a M13_pUC_fwd_pri M13_forward20_pri ..........

.......... .......... .......... .......... .......... ..........

3001 actata.... .......... .......... .......... .......... .......... 3006

3001 tgatat.... .......... .......... .......... .......... .......... 3006

.......... .......... .......... .......... .......... ..........