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Backbone size: 4870
GenBank Accession Number:D00054
Comments:PHY300PLK is one of the smallest hybrids of a new series of chimeric plasmids using the parental plasmids, pACYC177 of E.coli and pAM-alpha of Streptococcus faecalis. This shuttle vector for E.coli and B.subtilis contains an RNA primer gene for ori-177; the R-Tc gene; the R-Ap gene; Rep-alpha-1 gene, which is the plasmid replication gene; two replication origins, one for E.coli and the other for B. subtilis; and a polylinker. Twenty-three unique recognition restriction endonuclease sites have been found in pHY300PLK. Hosts: E.coli, B.subtilis, E.faecalis strain DS-5, S.faecalis. Related vectors: pACYC177, pAMalpha, pHY320PLK. (Information source: VectorDB.)