[5[5LOCUS pHcRed1-1 4118 bp DNA SYN 12-Sep-2007 DEFINITION pHcRed1-1 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4118 /organism="pHcRed1-1" /mol_type="other DNA" CDS 97..783 /label="ORF frame 1" /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRI HVTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGI LTAHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNV MALKVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVA RYSDLPEKAN*" misc_feature 997..1016 /label="EBV_rev_primer" rep_origin complement(1166..1472) /label="f1_origin" promoter 1551..1579 /label="AmpR_promoter" misc_feature complement(1645..1665) /label="pBABE_3_primer" misc_feature complement(1651..1866) /label="SV40_enhancer" promoter 1663..1931 /label="SV40_promoter" rep_origin 1830..1907 /label="SV40_origin" misc_feature 1892..1911 /label="SV40pro_F_primer" CDS 2014..2808 /label="Neomycin" /gene="Neomycin" /note="ORF frame 1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 2017..2805 /label="Neomycin" /gene="Neomycin" terminator 2983..3252 /label="TK_PA_terminator" rep_origin 3400..4019 /label="pBR322_origin" ORIGIN 1 TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA AGCTTCGAAT TCTGCAGTCG 61 ACGGTACCGC GGGCCCGGGA TCCACCGGTC GCCACCATGG TGAGCGGCCT GCTGAAGGAG 121 AGTATGCGCA TCAAGATGTA CATGGAGGGC ACCGTGAACG GCCACTACTT CAAGTGCGAG 181 GGCGAGGGCG ACGGCAACCC CTTCGCCGGC ACCCAGAGCA TGAGAATCCA CGTGACCGAG 241 GGCGCCCCCC TGCCCTTCGC CTTCGACATC CTGGCCCCCT GCTGCGAGTA CGGCAGCAGG 301 ACCTTCGTGC ACCACACCGC CGAGATCCCC GACTTCTTCA AGCAGAGCTT CCCCGAGGGC 361 TTCACCTGGG AGAGAACCAC CACCTACGAG GACGGCGGCA TCCTGACCGC CCACCAGGAC 421 ACCAGCCTGG AGGGCAACTG CCTGATCTAC AAGGTGAAGG TGCACGGCAC CAACTTCCCC 481 GCCGACGGCC CCGTGATGAA GAACAAGAGC GGCGGCTGGG AGCCCAGCAC CGAGGTGGTG 541 TACCCCGAGA ACGGCGTGCT GTGCGGCCGG AACGTGATGG CCCTGAAGGT GGGCGACCGG 601 CACCTGATCT GCCACCACTA CACCAGCTAC CGGAGCAAGA AGGCCGTGCG CGCCCTGACC 661 ATGCCCGGCT TCCACTTCAC CGACATCCGG CTCCAGATGC TGCGGAAGAA GAAGGACGAG 721 TACTTCGAGC TGTACGAGGC CAGCGTGGCC CGGTACAGCG ACCTGCCCGA GAAGGCCAAC 781 TGAAGCGGCC GCGACTCTAG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG 841 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG 901 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT 961 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG 1021 TATCTTAAGG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT 1081 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA 1141 GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG 1201 AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT 1261 GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC 1321 CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG 1381 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG 1441 CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC AGGTGGCACT 1501 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG 1561 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT 1621 CCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 1681 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 1741 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 1801 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 1861 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 1921 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG 1981 ATCGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA 2041 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC 2101 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC 2161 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG 2221 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG 2281 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT 2341 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT 2401 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA 2461 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA 2521 CTGTTCGCCA GGCTCAAGGC GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC 2581 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT 2641 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT 2701 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC 2761 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG 2821 GGTTCGAAAT GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG 2881 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 2941 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC TAGGGGGAGG CTAACTGAAA 3001 CACGGAAGGA GACAATACCG GAAGGAACCC GCGCTATGAC GGCAATAAAA AGACAGAATA 3061 AAACGCACGG TGTTGGGTCG TTTGTTCATA AACGCGGGGT TCGGTCCCAG GGCTGGCACT 3121 CTGTCGATAC CCCACCGAGA CCCCATTGGG GCCAATACGC CCGCGTTTCT TCCTTTTCCC 3181 CACCCCACCC CCCAAGTTCG GGTGAAGGCC CAGGGCTCGC AGCCAACGTC GGGGCGGCAG 3241 GCCCTGCCAT AGCCTCAGGT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 3301 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 3361 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 3421 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 3481 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 3541 GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 3601 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 3661 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 3721 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 3781 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3841 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3901 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3961 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 4021 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT 4081 CCCCTGATTC TGTGGATAAC CGTATTACCG CCATGCAT //  pHcRed1-1 "Times New Roman[5CaORF 1"Times New Roman[5C EBV_rev_pri"Times New Roman[5CɆ f1_origin"Times New Roman[5+AmpR_pro"Times New Roman[5Cm pBABE_3_pri"Times New Roman[5CsJSV40_enh"Times New Roman[5CɆSV40_pro"Times New Roman*[5&s SV40_origin"Times New Roman[5Cdw SV40pro_F_pri"Times New Roman[5C ORF 1"Times New Roman[5CɆ Neomycin"Times New Roman[5  TK_PA_term"Times New Roman[5CH  pBR322_origin"Times New Roman [5{BglII(27),XhoI(31),SacI(38),HindIII(40),EcoRI(47),PstI(56),SalI(57),KpnI(67),SacII(70),ApaI(75),SmaI(76),BamHI(78),AgeI(84)"Times New Roman3E [5PmlI"Times New Roman99' [5NotI(786),XbaI(796),HpaI(905)"Times New RomanLT]]- [5AflII"Times New RomanYdbdjcj\j [5StuI(1963),ClaI(1982)"Times New Roman2;CC [5MscI"Times New RomanY-U5S=2=tL [5test"Times New Roman #55