Sequence of pHcRed1-1

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pHcRed1-1

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pHcRed1-1 : 4118 BP; 970 A; 1134 C; 1114 G; 900 T; 0 other;

.......... ..... BglII(27),XhoI(31) SacI(38),HindIII(40) EcoRI(47)

1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 60

1 atcaataatg atcgcgatgg cctgagtcta gagctcgagt tcgaagctta agacgtcagc 60

.......... .......... .......... .......... .......... ..........

KpnI,SacII0),ApaI(75),SmaI(76),BamHI(78) AgeI(84) ....

61 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcggcct gctgaaggag 120

61 tgccatggcg cccgggccct aggtggccag cggtggtacc actcgccgga cgacttcctc 120

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

121 agtatgcgca tcaagatgta catggagggc accgtgaacg gccactactt caagtgcgag 180

121 tcatacgcgt agttctacat gtacctcccg tggcacttgc cggtgatgaa gttcacgctc 180

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(231)

181 ggcgagggcg acggcaaccc cttcgccggc acccagagca tgagaatcca cgtgaccgag 240

181 ccgctcccgc tgccgttggg gaagcggccg tgggtctcgt actcttaggt gcactggctc 240

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcgcccccc tgcccttcgc cttcgacatc ctggccccct gctgcgagta cggcagcagg 300

241 ccgcgggggg acgggaagcg gaagctgtag gaccggggga cgacgctcat gccgtcgtcc 300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 accttcgtgc accacaccgc cgagatcccc gacttcttca agcagagctt ccccgagggc 360

301 tggaagcacg tggtgtggcg gctctagggg ctgaagaagt tcgtctcgaa ggggctcccg 360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttcacctggg agagaaccac cacctacgag gacggcggca tcctgaccgc ccaccaggac 420

361 aagtggaccc tctcttggtg gtggatgctc ctgccgccgt aggactggcg ggtggtcctg 420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 accagcctgg agggcaactg cctgatctac aaggtgaagg tgcacggcac caacttcccc 480

421 tggtcggacc tcccgttgac ggactagatg ttccacttcc acgtgccgtg gttgaagggg 480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gccgacggcc ccgtgatgaa gaacaagagc ggcggctggg agcccagcac cgaggtggtg 540

481 cggctgccgg ggcactactt cttgttctcg ccgccgaccc tcgggtcgtg gctccaccac 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 taccccgaga acggcgtgct gtgcggccgg aacgtgatgg ccctgaaggt gggcgaccgg 600

541 atggggctct tgccgcacga cacgccggcc ttgcactacc gggacttcca cccgctggcc 600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cacctgatct gccaccacta caccagctac cggagcaaga aggccgtgcg cgccctgacc 660

601 gtggactaga cggtggtgat gtggtcgatg gcctcgttct tccggcacgc gcgggactgg 660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atgcccggct tccacttcac cgacatccgg ctccagatgc tgcggaagaa gaaggacgag 720

661 tacgggccga aggtgaagtg gctgtaggcc gaggtctacg acgccttctt cttcctgctc 720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tacttcgagc tgtacgaggc cagcgtggcc cggtacagcg acctgcccga gaaggccaac 780

721 atgaagctcg acatgctccg gtcgcaccgg gccatgtcgc tggacgggct cttccggttg 780

.......... .......... ORF 1 .......... .......... ..........

NotI(786) .......... .......... .......... .......... ..........

781 tgaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 840

781 acttcgccgg cgctgagatc tagtattagt cggtatggtg taaacatctc caaaatgaac 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 900

841 gaaatttttt ggagggtgtg gagggggact tggactttgt attttactta cgttaacaac 900

.......... .......... .......... .......... .......... ..........

HpaI(905) .......... .......... .......... .......... ..........

901 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 960

901 aacaattgaa caaataacgt cgaatattac caatgtttat ttcgttatcg tagtgtttaa 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 1020

961 agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt gagtagttac 1020

.......... .......... .......... ......... EBV_rev_pri .........

AflII(1024) ........ .......... .......... .......... ..........

1021 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 1080

1021 atagaattcc gcatttaaca ttcgcaatta taaaacaatt ttaagcgcaa tttaaaaaca 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 1140

1081 atttagtcga gtaaaaaatt ggttatccgg ctttagccgt tttagggaat atttagtttt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 1200

1141 cttatctggc tctatcccaa ctcacaacaa ggtcaaacct tgttctcagg tgataatttc 1200

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

1201 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 1260

1201 ttgcacctga ggttgcagtt tcccgctttt tggcagatag tcccgctacc gggtgatgca 1260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 1320

1261 cttggtagtg ggattagttc aaaaaacccc agctccacgg catttcgtga tttagccttg 1320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 1380

1321 ggatttccct cgggggctaa atctcgaact gcccctttcg gccgcttgca ccgctctttc 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 1440

1381 cttcccttct ttcgctttcc tcgcccgcga tcccgcgacc gttcacatcg ccagtgcgac 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 1500

1441 gcgcattggt ggtgtgggcg gcgcgaatta cgcggcgatg tcccgcgcag tccaccgtga 1500

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 1560

1501 aaagcccctt tacacgcgcc ttggggataa acaaataaaa agatttatgt aagtttatac 1560

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 1620

1561 ataggcgagt actctgttat tgggactatt tacgaagtta ttataacttt ttccttctca 1620

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 1680

1621 ggactccgcc tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc 1680

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

1681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 1740

1681 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac 1740

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 1800

1741 ctttcagggg tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc 1800

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 1860

1801 gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 1860

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

1861 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 1920

1861 taagaggcgg ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc 1920

.......... ..... SV40_pro SV40_origin ... SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

1921 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 1980

1921 ggagactcga taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttc 1980

SV40_pro .......... .......... .......... .......... ..........

ClaI(1982) .......... .......... .......... .......... ..........

1981 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 2040

1981 tagctagttc tctgtcctac tcctagcaaa gcgtactaac ttgttctacc taacgtgcgt 2040

.......... .......... .......... ........ ORF 1 Neomycin ........

.......... .......... .......... .......... .......... ..........

2041 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 2100

2041 ccaagaggcc ggcgaaccca cctctccgat aagccgatac tgacccgtgt tgtctgttag 2100

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 2160

2101 ccgacgagac tacggcggca caaggccgac agtcgcgtcc ccgcgggcca agaaaaacag 2160

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 2220

2161 ttctggctgg acaggccacg ggacttactt gacgttctgc tccgtcgcgc cgatagcacc 2220

.......... ........ ORF 1 Neomycin ........ .......... ..........

MscI(2224) .......... .......... .......... .......... ..........

2221 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 2280

2221 gaccggtgct gcccgcaagg aacgcgtcga cacgagctgc aacagtgact tcgcccttcc 2280

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 2340

2281 ctgaccgacg ataacccgct tcacggcccc gtcctagagg acagtagagt ggaacgagga 2340

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 2400

2341 cggctctttc ataggtagta ccgactacgt tacgccgccg acgtatgcga actaggccga 2400

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 2460

2401 tggacgggta agctggtggt tcgctttgta gcgtagctcg ctcgtgcatg agcctacctt 2460

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 2520

2461 cggccagaac agctagtcct actagacctg cttctcgtag tccccgagcg cggtcggctt 2520

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 2580

2521 gacaagcggt ccgagttccg ctcgtacggg ctgccgctcc tagagcagca ctgggtaccg 2580

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 2640

2581 ctacggacga acggcttata gtaccacctt ttaccggcga aaagacctaa gtagctgaca 2640

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 2700

2641 ccggccgacc cacaccgcct ggcgatagtc ctgtatcgca accgatgggc actataacga 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 2760

2701 cttctcgaac cgccgcttac ccgactggcg aaggagcacg aaatgccata gcggcgaggg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 2820

2761 ctaagcgtcg cgtagcggaa gatagcggaa gaactgctca agaagactcg ccctgagacc 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 2880

2821 ccaagcttta ctggctggtt cgctgcgggt tggacggtag tgctctaaag ctaaggtggc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 2940

2881 ggcggaagat actttccaac ccgaagcctt agcaaaaggc cctgcggccg acctactagg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 3000

2941 aggtcgcgcc cctagagtac gacctcaaga agcgggtggg atccccctcc gattgacttt 3000

.......... .......... .......... .......... .......... TK_PA_term

.......... .......... .......... .......... .......... ..........

3001 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 3060

3001 gtgccttcct ctgttatggc cttccttggg cgcgatactg ccgttatttt tctgtcttat 3060

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 3120

3061 tttgcgtgcc acaacccagc aaacaagtat ttgcgcccca agccagggtc ccgaccgtga 3120

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 3180

3121 gacagctatg gggtggctct ggggtaaccc cggttatgcg ggcgcaaaga aggaaaaggg 3180

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 3240

3181 gtggggtggg gggttcaagc ccacttccgg gtcccgagcg tcggttgcag ccccgccgtc 3240

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 3300

3241 cgggacggta tcggagtcca atgagtatat atgaaatcta actaaatttt gaagtaaaaa 3300

TK_PA_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 3360

3301 ttaaattttc ctagatccac ttctaggaaa aactattaga gtactggttt tagggaattg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 3420

3361 cactcaaaag caaggtgact cgcagtctgg ggcatctttt ctagtttcct agaagaactc 3420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 3480

3421 taggaaaaaa agacgcgcat tagacgacga acgtttgttt ttttggtggc gatggtcgcc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 3540

3481 accaaacaaa cggcctagtt ctcgatggtt gagaaaaagg cttccattga ccgaagtcgt 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 3600

3541 ctcgcgtcta tggtttatga caggaagatc acatcggcat caatccggtg gtgaagttct 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 3660

3601 tgagacatcg tggcggatgt atggagcgag acgattagga caatggtcac cgacgacggt 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 3720

3661 caccgctatt cagcacagaa tggcccaacc tgagttctgc tatcaatggc ctattccgcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 3780

3721 tcgccagccc gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct tgctggatgt 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 3840

3781 ggcttgactc tatggatgtc gcactcgata ctctttcgcg gtgcgaaggg cttccctctt 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3900

3841 tccgcctgtc cataggccat tcgccgtccc agccttgtcc tctcgcgtgc tccctcgaag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3960

3901 gtcccccttt gcggaccata gaaatatcag gacagcccaa agcggtggag actgaactcg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 4020

3961 cagctaaaaa cactacgagc agtccccccg cctcggatac ctttttgcgg tcgttgcgcc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 4080

4021 ggaaaaatgc caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa ggacgcaata 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccctgattc tgtggataac cgtattaccg ccatgcat.. .......... .......... 4118

4081 ggggactaag acacctattg gcataatggc ggtacgta.. .......... .......... 4118

.......... .......... .......... .......... .......... ..........