Sequence of pHcRed1-C1

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Sequence of pHcRed1-C1

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Sequence of pHcRed1-C1 : 4698 BP; 1117 A; 1276 C; 1251 G; 1054 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... ..... NheI(591) AgeI(600)

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccggtcgcca ccatggtgag cggcctgctg aaggagagta tgcgcatcaa gatgtacatg 660

601 ggccagcggt ggtaccactc gccggacgac ttcctctcat acgcgtagtt ctacatgtac 660

.......... .......... ORF 1 ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

661 gagggcaccg tgaacggcca ctacttcaag tgcgagggcg agggcgacgg caaccccttc 720

661 ctcccgtggc acttgccggt gatgaagttc acgctcccgc tcccgctgcc gttggggaag 720

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... PmlI(747) .......... .......... ..........

721 gccggcaccc agagcatgag aatccacgtg accgagggcg cccccctgcc cttcgccttc 780

721 cggccgtggg tctcgtactc ttaggtgcac tggctcccgc ggggggacgg gaagcggaag 780

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gacatcctgg ccccctgctg cgagtacggc agcaggacct tcgtgcacca caccgccgag 840

781 ctgtaggacc gggggacgac gctcatgccg tcgtcctgga agcacgtggt gtggcggctc 840

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atccccgact tcttcaagca gagcttcccc gagggcttca cctgggagag aaccaccacc 900

841 taggggctga agaagttcgt ctcgaagggg ctcccgaagt ggaccctctc ttggtggtgg 900

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tacgaggacg gcggcatcct gaccgcccac caggacacca gcctggaggg caactgcctg 960

901 atgctcctgc cgccgtagga ctggcgggtg gtcctgtggt cggacctccc gttgacggac 960

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atctacaagg tgaaggtgca cggcaccaac ttccccgccg acggccccgt gatgaagaac 1020

961 tagatgttcc acttccacgt gccgtggttg aaggggcggc tgccggggca ctacttcttg 1020

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aagagcggcg gctgggagcc cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc 1080

1021 ttctcgccgc cgaccctcgg gtcgtggctc caccacatgg ggctcttgcc gcacgacacg 1080

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggccggaacg tgatggccct gaaggtgggc gaccggcacc tgatctgcca ccactacacc 1140

1081 ccggccttgc actaccggga cttccacccg ctggccgtgg actagacggt ggtgatgtgg 1140

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agctaccgga gcaagaaggc cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac 1200

1141 tcgatggcct cgttcttccg gcacgcgcgg gactggtacg ggccgaaggt gaagtggctg 1200

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atccggctcc agatgctgcg gaagaagaag gacgagtact tcgagctgta cgaggccagc 1260

1201 taggccgagg tctacgacgc cttcttcttc ctgctcatga agctcgacat gctccggtcg 1260

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .... BglII(1306),XhoI(1310) ....

1261 gtggcccggt acagcgacct gcccgagaag gccaactccg gactcagatc tcgagctcaa 1320

1261 caccgggcca tgtcgctgga cgggctcttc cggttgaggc ctgagtctag agctcgagtt 1320

.......... ......... ORF 1 ORF 1 ......... .......... ..........

EcoRI PstI,SalI KpnI,SacII,ApaI,SmaI,BamHI XbaI BclI

1321 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg 1380

1321 cgaagcttaa gacgtcagct gccatggcgc ccgggcccta ggtggcctag atctattgac 1380

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac 1440

1381 tagtattagt cggtatggtg taaacatctc caaaatgaac gaaatttttt ggagggtgtg 1440

ORF 1 ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... HpaI(1485) ..........

1441 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca 1500

1441 gagggggact tggactttgt attttactta cgttaacaac aacaattgaa caaataacgt 1500

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 1560

1501 cgaatattac caatgtttat ttcgttatcg tagtgtttaa agtgtttatt tcgtaaaaaa 1560

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt 1620

1561 agtgacgtaa gatcaacacc aaacaggttt gagtagttac atagaattgc gcatttaaca 1620

.......... ... SV40_PA_term EBV_rev_pri ... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 1680

1621 ttcgcaatta taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 1740

1681 ggttatccgg ctttagccgt tttagggaat atttagtttt cttatctggc tctatcccaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 1800

1741 ctcacaacaa ggtcaaacct tgttctcagg tgataatttc ttgcacctga ggttgcagtt 1800

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 1860

1801 tcccgctttt tggcagatag tcccgctacc gggtgatgca cttggtagtg ggattagttc 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 1920

1861 aaaaaacccc agctccacgg catttcgtga tttagccttg ggatttccct cgggggctaa 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 1980

1921 atctcgaact gcccctttcg gccgcttgca ccgctctttc cttcccttct ttcgctttcc 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 2040

1981 tcgcccgcga tcccgcgacc gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg 2100

2041 gcgcgaatta cgcggcgatg tcccgcgcag tccaccgtga aaagcccctt tacacgcgcc 2100

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 2160

2101 ttggggataa acaaataaaa agatttatgt aagtttatac ataggcgagt actctgttat 2160

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2161 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag 2220

2161 tgggactatt tacgaagtta ttataacttt ttccttctca ggactccgcc tttcttggtc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 2280

2221 gacaccttac acacagtcaa tcccacacct ttcaggggtc cgaggggtcg tccgtcttca 2280

......... pBABE_3_pri ......... SV40_enh SV40_pro ..........

.......... .......... .......... .......... .......... ..........

2281 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 2340

2281 tacgtttcgt acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 2400

2341 cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 2460

2401 tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact 2460

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 2520

2461 gattaaaaaa aataaatacg tctccggctc cggcggagcc ggagactcga taaggtcttc 2520

... SV40_origin SV40_pro SV40pro_F_pri ... .......... ..........

.......... .......... StuI(2543) .......... ClaI(2562) ..........

2521 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg 2580

2521 atcactcctc cgaaaaaacc tccggatccg aaaacgtttc tagctagttc tctgtcctac 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 2640

2581 tcctagcaaa gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc ggcgaaccca 2640

.......... .......... ........ ORF 2 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

2641 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 2700

2641 cctctccgat aagccgatac tgacccgtgt tgtctgttag ccgacgagac tacggcggca 2700

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 2760

2701 caaggccgac agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg acaggccacg 2760

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... MscI(2804) ..........

2761 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 2820

2761 ggacttactt gacgttctgc tccgtcgcgc cgatagcacc gaccggtgct gcccgcaagg 2820

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 2880

2821 aacgcgtcga cacgagctgc aacagtgact tcgcccttcc ctgaccgacg ataacccgct 2880

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 2940

2881 tcacggcccc gtcctagagg acagtagagt ggaacgagga cggctctttc ataggtagta 2940

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 3000

2941 ccgactacgt tacgccgccg acgtatgcga actaggccga tggacgggta agctggtggt 3000

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 3060

3001 tcgctttgta gcgtagctcg ctcgtgcatg agcctacctt cggccagaac agctagtcct 3060

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 3120

3061 actagacctg cttctcgtag tccccgagcg cggtcggctt gacaagcggt ccgagttccg 3120

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 3180

3121 ctcgtacggg ctgccgctcc tagagcagca ctgggtaccg ctacggacga acggcttata 3180

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 3240

3181 gtaccacctt ttaccggcga aaagacctaa gtagctgaca ccggccgacc cacaccgcct 3240

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 3300

3241 ggcgatagtc ctgtatcgca accgatgggc actataacga cttctcgaac cgccgcttac 3300

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 3360

3301 ccgactggcg aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg cgtagcggaa 3360

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 3420

3361 gatagcggaa gaactgctca agaagactcg ccctgagacc ccaagcttta ctggctggtt 3420

........ ORF 2 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 3480

3421 cgctgcgggt tggacggtag tgctctaaag ctaaggtggc ggcggaagat actttccaac 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 3540

3481 ccgaagcctt agcaaaaggc cctgcggccg acctactagg aggtcgcgcc cctagagtac 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg 3600

3541 gacctcaaga agcgggtggg atccccctcc gattgacttt gtgccttcct ctgttatggc 3600

.......... .......... .......... .......... TK_PA_term ..........

.......... .......... .......... .......... .......... ..........

3601 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg 3660

3601 cttccttggg cgcgatactg ccgttatttt tctgtcttat tttgcgtgcc acaacccagc 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga 3720

3661 aaacaagtat ttgcgcccca agccagggtc ccgaccgtga gacagctatg gggtggctct 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg 3780

3721 ggggtaaccc cggttatgcg ggcgcaaaga aggaaaaggg gtggggtggg gggttcaagc 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt 3840

3781 ccacttccgg gtcccgagcg tcggttgcag ccccgccgtc cgggacggta tcggagtcca 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 3900

3841 atgagtatat atgaaatcta actaaatttt gaagtaaaaa ttaaattttc ctagatccac 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 3960

3901 ttctaggaaa aactattaga gtactggttt tagggaattg cactcaaaag caaggtgact 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 4020

3961 cgcagtctgg ggcatctttt ctagtttcct agaagaactc taggaaaaaa agacgcgcat 4020

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4021 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 4080

4021 tagacgacga acgtttgttt ttttggtggc gatggtcgcc accaaacaaa cggcctagtt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 4140

4081 ctcgatggtt gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta tggtttatga 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 4200

4141 caggaagatc acatcggcat caatccggtg gtgaagttct tgagacatcg tggcggatgt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 4260

4201 atggagcgag acgattagga caatggtcac cgacgacggt caccgctatt cagcacagaa 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 4320

4261 tggcccaacc tgagttctgc tatcaatggc ctattccgcg tcgccagccc gacttgcccc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 4380

4321 ccaagcacgt gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc tatggatgtc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 4440

4381 gcactcgata ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc cataggccat 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 4500

4441 tcgccgtccc agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt gcggaccata 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 4560

4501 gaaatatcag gacagcccaa agcggtggag actgaactcg cagctaaaaa cactacgagc 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 4620

4561 agtccccccg cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc caaggaccgg 4620

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 4680

4621 aaaacgaccg gaaaacgagt gtacaagaaa ggacgcaata ggggactaag acacctattg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgtattaccg ccatgcat.. .......... .......... .......... .......... 4698

4681 gcataatggc ggtacgta.. .......... .......... .......... .......... 4698

.......... .......... .......... .......... .......... ..........