LOCUS pHcRed1-N1/1 4700 bp DNA SYN 13-Sep-2007 DEFINITION pHcRed1-N1/1 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4700 /organism="pHcRed1-N1/1" /mol_type="other DNA" misc_feature 65..352 /label="CAG_enhancer" promoter 10..562 /label="CMV_immearly_promoter" misc_feature 519..539 /label="CMV_fwd_primer" promoter 520..589 /label="CMV_promoter" CDS 679..1365 /label="ORF frame 1" /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRI HVTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGI LTAHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNV MALKVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVA RYSDLPEKAN*" misc_feature 1579..1598 /label="EBV_rev_primer" rep_origin complement(1748..2054) /label="f1_origin" promoter 2133..2161 /label="AmpR_promoter" misc_feature complement(2227..2247) /label="pBABE_3_primer" misc_feature complement(2233..2448) /label="SV40_enhancer" promoter 2245..2513 /label="SV40_promoter" rep_origin 2412..2489 /label="SV40_origin" misc_feature 2474..2493 /label="SV40pro_F_primer" CDS 2596..3390 /label="Neomycin" /gene="Neomycin" /note="ORF frame 1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" gene 2599..3387 /label="Neomycin" /gene="Neomycin" terminator 3565..3834 /label="TK_PA_terminator" rep_origin 3982..4601 /label="pBR322_origin" ORIGIN 1 TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG 61 CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT 121 GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA 181 ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC 241 AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA 301 CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC 361 CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG 421 ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG 481 GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT 541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCGG 661 GATCCACCGG TCGCCACCAT GGTGAGCGGC CTGCTGAAGG AGAGTATGCG CATCAAGATG 721 TACATGGAGG GCACCGTGAA CGGCCACTAC TTCAAGTGCG AGGGCGAGGG CGACGGCAAC 781 CCCTTCGCCG GCACCCAGAG CATGAGAATC CACGTGACCG AGGGCGCCCC CCTGCCCTTC 841 GCCTTCGACA TCCTGGCCCC CTGCTGCGAG TACGGCAGCA GGACCTTCGT GCACCACACC 901 GCCGAGATCC CCGACTTCTT CAAGCAGAGC TTCCCCGAGG GCTTCACCTG GGAGAGAACC 961 ACCACCTACG AGGACGGCGG CATCCTGACC GCCCACCAGG ACACCAGCCT GGAGGGCAAC 1021 TGCCTGATCT ACAAGGTGAA GGTGCACGGC ACCAACTTCC CCGCCGACGG CCCCGTGATG 1081 AAGAACAAGA GCGGCGGCTG GGAGCCCAGC ACCGAGGTGG TGTACCCCGA GAACGGCGTG 1141 CTGTGCGGCC GGAACGTGAT GGCCCTGAAG GTGGGCGACC GGCACCTGAT CTGCCACCAC 1201 TACACCAGCT ACCGGAGCAA GAAGGCCGTG CGCGCCCTGA CCATGCCCGG CTTCCACTTC 1261 ACCGACATCC GGCTCCAGAT GCTGCGGAAG AAGAAGGACG AGTACTTCGA GCTGTACGAG 1321 GCCAGCGTGG CCCGGTACAG CGACCTGCCC GAGAAGGCCA ACTGAAGCGG CCGCGACTCT 1381 AGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC 1441 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG 1501 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 1561 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA GGCGTAAATT 1621 GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT 1681 AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG 1741 TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC 1801 AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA 1861 AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA 1921 TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA 1981 GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC 2041 GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC 2101 GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA 2161 TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTCCTGAGGC GGAAAGAACC 2221 AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 2281 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC 2341 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC 2401 TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT 2461 GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA 2521 AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA 2581 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 2641 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 2701 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 2761 GCCCTGAATG AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 2821 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 2881 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 2941 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 3001 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 3061 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 3121 GCGAGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 3181 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG 3241 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 3301 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 3361 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC 3421 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT 3481 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA 3541 TGCTGGAGTT CTTCGCCCAC CCTAGGGGGA GGCTAACTGA AACACGGAAG GAGACAATAC 3601 CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC GGTGTTGGGT 3661 CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA CTCTGTCGAT ACCCCACCGA 3721 GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC CCCACCCCAC CCCCCAAGTT 3781 CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC AGGCCCTGCC ATAGCCTCAG 3841 GTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG 3901 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT 3961 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 4021 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC 4081 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA 4141 CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA 4201 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC 4261 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 4321 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC 4381 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG 4441 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT 4501 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT 4561 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 4621 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA 4681 ACCGTATTAC CGCCATGCAT //\pHcRed1-N1/1 "Times New RomanC\A`CAG_enh"Times New RomanC\ 2CMV_immearly_pro"Times New RomanCɆ\ CMV_fwd_pri"Times New Roman\MCMV_pro"Times New Roman(C\UORF 1"Times New RomanC\+> EBV_rev_pri"Times New RomanCɆ\ f1_origin"Times New Roman\UqAmpR_pro"Times New RomanC\ pBABE_3_pri"Times New RomanC\ SV40_enh"Times New RomanCɆ\ SV40_pro"Times New Roman*\l  SV40_origin"Times New RomanC\  SV40pro_F_pri"Times New RomanC\$ > ORF 1"Times New RomanCɆ\' ; Neomycin"Times New Roman\  TK_PA_term"Times New RomanC\ pBR322_origin"Times New Roman AseI"Times New Roman\ NdeI"Times New Roman\ ͒NheI(591),BglII(609),XhoI(613),SacI(620),HindIII(622),EcoRI(629),PstI(638),SalI(639),KpnI(649),SacII(652),ApaI(657),SmaI(658),BamHI(660),AgeI(666)"Times New RomanO\@GN N:  PmlI"Times New Roman-\iRqMyHy9H  NotI(1368),XbaI(1378),HpaI(1487)"Times New RomanX\} AflII"Times New RomanF\^;g@oEoET StuI(2545),ClaI(2564)"Times New Roman \fc`Qo MscI"Times New Roman \| test"Times New Roman+\$U$\! test"Times New Roman \B