Sequence of pHcRed1-N1

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Sequence of pHcRed1-N1_1

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Sequence of pHcRed1-N1/1 : 4700 BP; 1116 A; 1277 C; 1255 G; 1052 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI,HindIII EcoRI PstI(638),SalI(639)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

AgeI(666) .......... .......... .......... .......... ..........

661 gatccaccgg tcgccaccat ggtgagcggc ctgctgaagg agagtatgcg catcaagatg 720

661 ctaggtggcc agcggtggta ccactcgccg gacgacttcc tctcatacgc gtagttctac 720

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

721 tacatggagg gcaccgtgaa cggccactac ttcaagtgcg agggcgaggg cgacggcaac 780

721 atgtacctcc cgtggcactt gccggtgatg aagttcacgc tcccgctccc gctgccgttg 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... PmlI(813) .......... ..........

781 cccttcgccg gcacccagag catgagaatc cacgtgaccg agggcgcccc cctgcccttc 840

781 gggaagcggc cgtgggtctc gtactcttag gtgcactggc tcccgcgggg ggacgggaag 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gccttcgaca tcctggcccc ctgctgcgag tacggcagca ggaccttcgt gcaccacacc 900

841 cggaagctgt aggaccgggg gacgacgctc atgccgtcgt cctggaagca cgtggtgtgg 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gccgagatcc ccgacttctt caagcagagc ttccccgagg gcttcacctg ggagagaacc 960

901 cggctctagg ggctgaagaa gttcgtctcg aaggggctcc cgaagtggac cctctcttgg 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 accacctacg aggacggcgg catcctgacc gcccaccagg acaccagcct ggagggcaac 1020

961 tggtggatgc tcctgccgcc gtaggactgg cgggtggtcc tgtggtcgga cctcccgttg 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgcctgatct acaaggtgaa ggtgcacggc accaacttcc ccgccgacgg ccccgtgatg 1080

1021 acggactaga tgttccactt ccacgtgccg tggttgaagg ggcggctgcc ggggcactac 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aagaacaaga gcggcggctg ggagcccagc accgaggtgg tgtaccccga gaacggcgtg 1140

1081 ttcttgttct cgccgccgac cctcgggtcg tggctccacc acatggggct cttgccgcac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctgtgcggcc ggaacgtgat ggccctgaag gtgggcgacc ggcacctgat ctgccaccac 1200

1141 gacacgccgg ccttgcacta ccgggacttc cacccgctgg ccgtggacta gacggtggtg 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tacaccagct accggagcaa gaaggccgtg cgcgccctga ccatgcccgg cttccacttc 1260

1201 atgtggtcga tggcctcgtt cttccggcac gcgcgggact ggtacgggcc gaaggtgaag 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 accgacatcc ggctccagat gctgcggaag aagaaggacg agtacttcga gctgtacgag 1320

1261 tggctgtagg ccgaggtcta cgacgccttc ttcttcctgc tcatgaagct cgacatgctc 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... NotI(1368) ..........

1321 gccagcgtgg cccggtacag cgacctgccc gagaaggcca actgaagcgg ccgcgactct 1380

1321 cggtcgcacc gggccatgtc gctggacggg ctcttccggt tgacttcgcc ggcgctgaga 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 1440

1381 tctagtatta gtcggtatgg tgtaaacatc tccaaaatga acgaaatttt ttggagggtg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... HpaI(1487) ..........

1441 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 1500

1441 tggaggggga cttggacttt gtattttact tacgttaaca acaacaattg aacaaataac 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 1560

1501 gtcgaatatt accaatgttt atttcgttat cgtagtgttt aaagtgttta tttcgtaaaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AflII(1606) .........

1561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt 1620

1561 aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt acatagaatt ccgcatttaa 1620

.......... ......... EBV_rev_pri ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 1680

1621 cattcgcaat tataaaacaa ttttaagcgc aatttaaaaa caatttagtc gagtaaaaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 1740

1681 ttggttatcc ggctttagcc gttttaggga atatttagtt ttcttatctg gctctatccc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 1800

1741 aactcacaac aaggtcaaac cttgttctca ggtgataatt tcttgcacct gaggttgcag 1800

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 1860

1801 tttcccgctt tttggcagat agtcccgcta ccgggtgatg cacttggtag tgggattagt 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 1920

1861 tcaaaaaacc ccagctccac ggcatttcgt gatttagcct tgggatttcc ctcgggggct 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 1980

1921 aaatctcgaa ctgccccttt cggccgcttg caccgctctt tccttccctt ctttcgcttt 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 2040

1981 cctcgcccgc gatcccgcga ccgttcacat cgccagtgcg acgcgcattg gtggtgtggg 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 2100

2041 cggcgcgaat tacgcggcga tgtcccgcgc agtccaccgt gaaaagcccc tttacacgcg 2100

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 2160

2101 ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga gtactctgtt 2160

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2161 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 2220

2161 attgggacta tttacgaagt tattataact ttttccttct caggactccg cctttcttgg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 2280

2221 tcgacacctt acacacagtc aatcccacac ctttcagggg tccgaggggt cgtccgtctt 2280

......... pBABE_3_pri ......... SV40_enh SV40_pro ..........

.......... .......... .......... .......... .......... ..........

2281 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 2340

2281 catacgtttc gtacgtagag ttaatcagtc gttggtccac acctttcagg ggtccgaggg 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 2400

2341 gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtat cagggcgggg 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 2460

2401 attgaggcgg gtagggcggg gattgaggcg ggtcaaggcg ggtaagaggc ggggtaccga 2460

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2461 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 2520

2461 ctgattaaaa aaaataaata cgtctccggc tccggcggag ccggagactc gataaggtct 2520

... SV40_origin SV40_pro SV40pro_F_pri ... .......... ..........

.......... .......... StuI(2545) .......... ClaI(2564) ..........

2521 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 2580

2521 tcatcactcc tccgaaaaaa cctccggatc cgaaaacgtt tctagctagt tctctgtcct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 2640

2581 actcctagca aagcgtacta acttgttcta cctaacgtgc gtccaagagg ccggcgaacc 2640

.......... .......... ........ ORF 1 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

2641 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 2700

2641 cacctctccg ataagccgat actgacccgt gttgtctgtt agccgacgag actacggcgg 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 2760

2701 cacaaggccg acagtcgcgt ccccgcgggc caagaaaaac agttctggct ggacaggcca 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... MscI(2806) ..........

2761 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 2820

2761 cgggacttac ttgacgttct gctccgtcgc gccgatagca ccgaccggtg ctgcccgcaa 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 2880

2821 ggaacgcgtc gacacgagct gcaacagtga cttcgccctt ccctgaccga cgataacccg 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 2940

2881 cttcacggcc ccgtcctaga ggacagtaga gtggaacgag gacggctctt tcataggtag 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 3000

2941 taccgactac gttacgccgc cgacgtatgc gaactaggcc gatggacggg taagctggtg 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 3060

3001 gttcgctttg tagcgtagct cgctcgtgca tgagcctacc ttcggccaga acagctagtc 3060

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 3120

3061 ctactagacc tgcttctcgt agtccccgag cgcggtcggc ttgacaagcg gtccgagttc 3120

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 3180

3121 cgctcgtacg ggctgccgct cctagagcag cactgggtac cgctacggac gaacggctta 3180

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3240

3181 tagtaccacc ttttaccggc gaaaagacct aagtagctga caccggccga cccacaccgc 3240

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3300

3241 ctggcgatag tcctgtatcg caaccgatgg gcactataac gacttctcga accgccgctt 3300

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3360

3301 acccgactgg cgaaggagca cgaaatgcca tagcggcgag ggctaagcgt cgcgtagcgg 3360

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3420

3361 aagatagcgg aagaactgct caagaagact cgccctgaga ccccaagctt tactggctgg 3420

........ ORF 1 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3480

3421 ttcgctgcgg gttggacggt agtgctctaa agctaaggtg gcggcggaag atactttcca 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3540

3481 acccgaagcc ttagcaaaag gccctgcggc cgacctacta ggaggtcgcg cccctagagt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 3600

3541 acgacctcaa gaagcgggtg ggatccccct ccgattgact ttgtgccttc ctctgttatg 3600

.......... .......... .......... .......... TK_PA_term ..........

.......... .......... .......... .......... .......... ..........

3601 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 3660

3601 gccttccttg ggcgcgatac tgccgttatt tttctgtctt attttgcgtg ccacaaccca 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3720

3661 gcaaacaagt atttgcgccc caagccaggg tcccgaccgt gagacagcta tggggtggct 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3780

3721 ctggggtaac cccggttatg cgggcgcaaa gaaggaaaag gggtggggtg gggggttcaa 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 3840

3781 gcccacttcc gggtcccgag cgtcggttgc agccccgccg tccgggacgg tatcggagtc 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 3900

3841 caatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt tcctagatcc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 3960

3901 acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa agcaaggtga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 4020

3961 ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc 4020

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 4080

4021 attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 4140

4081 ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 4200

4141 gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 4260

4201 gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 4320

4261 aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 4380

4321 ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 4440

4381 tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 4500

4441 attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 4560

4501 tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 4620

4561 gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 4680

4621 ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta agacacctat 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 accgtattac cgccatgcat .......... .......... .......... .......... 4700

4681 tggcataatg gcggtacgta .......... .......... .......... .......... 4700

.......... .......... .......... .......... .......... ..........