Sequence of pIB-E

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Sequence of pIB-E_Echo

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Sequence of pIB-E Echo : 2537 BP; 632 A; 659 C; 609 G; 637 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac 60

1 cctagtacta ctatttgtta cataccacga ttacaacgaa gttgttgtta agacaacttg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt 120

61 acacaaaagt acaaacggtt gttcgtggaa atatgagcca ccggaggggt ggtggttgaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(158) .......... ..........

121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg 180

121 aaaacgtgac gtttttttgt gcgaaaacgt gcgcccgggt atgtatcatg tttgagatgc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc 240

181 aaagcatctg ataaaatgta tttatcagat gtggcaacat atgcgaggtt tatgtgatgg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct 300

241 tgtgtaactt ggaaaaacgt cacgtttttt catgcacagc cgtcagtgca tccggccgga 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta 360

301 atagcccagc gcaggacagt gcatgcttag tgtaatagcc tggcctgctc acaacagaat 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg 420

361 agcactgtcc tgcggtcgaa ggacacaacg attggcgtcg gcctgcgttg aggaatagcc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc 480

421 ttgtcctgcg cggaggtata gtcggcgcgc aatagagtac gcgcactggc ctgtgctccg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga 540

481 cgggcagggc gaatagcgcg gatatttatg tcgggcgttg ctagaccatt tgtgtcaact 540

.......... .......... .......... ........ OpIE2_fwd_pri ........

.......... BstBI(552) .......... .......... .......... ..........

541 acagcatctg ttcgaattta aagcttatta cctcatatag catacattat acgaagttat 600

541 tgtcgtagac aagcttaaat ttcgaataat ggagtatatc gtatgtaata tgcttcaata 600

.......... .......... .......... .......... loxH ..........

EcoRV(603) .......... .......... .......... .......... ..........

601 gatatctgac taaatcttag tttgtattgt catgttttaa tacaatatgt tatgtttaaa 660

601 ctatagactg atttagaatc aaacataaca gtacaaaatt atgttataca atacaaattt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tatgttttta ataaatttta taaaataatt tcaactttta ttgtaacaac attgtccatt 720

661 atacaaaaat tatttaaaat attttattaa agttgaaaat aacattgttg taacaggtaa 720

.......... .......... .......... .......... .......... ..........

.......... .......... ClaI(748) .......... .......... ..........

721 tacacactcc tttcaagcgc gtgcggatcg atgctcactc aaaggcggta atacggttat 780

721 atgtgtgagg aaagttcgcg cacgcctagc tacgagtgag tttccgccat tatgccaata 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 840

781 ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc gttttccggt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 900

841 ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg ggactgctcg 900

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 960

901 tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat atttctatgg 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 1020

961 tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac ggcgaatggc 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctctatcc tcacgctgta 1080

1021 ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagatagg agtgcgacat 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... ......... ApaLI(1125) .........

1081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 1140

1081 ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg cttggggggc 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 1200

1141 aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg ggccattctg 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 1260

1201 tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc tccatacatc 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 1320

1261 cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct tcttgtcata 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1380

1321 aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca tcgagaacta 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1440

1381 ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc gtctaatgcg 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1500

1441 cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga ctgcgagtca 1500

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggaacgaaaa ctcacgttaa gggattttgg tcatgcgaaa cacgcacggc gcgcgcacgc 1560

1501 ccttgctttt gagtgcaatt ccctaaaacc agtacgcttt gtgcgtgccg cgcgcgtgcg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agcttagcac aaacgcgtcg ttgcacgcgc ccaccgctaa ccgcaggcca atcggtcggc 1620

1561 tcgaatcgtg tttgcgcagc aacgtgcgcg ggtggcgatt ggcgtccggt tagccagccg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cggcctcata tccgctcacc agccgcgtcc tatcgggcgc ggcttccgcg cccattttga 1680

1621 gccggagtat aggcgagtgg tcggcgcagg atagcccgcg ccgaaggcgc gggtaaaact 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ataaataaac gataacgccg ttggtggcgt gaggcatgta aaaggttaca tcattatctt 1740

1681 tatttatttg ctattgcggc aaccaccgca ctccgtacat tttccaatgt agtaatagaa 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gttcgccatc cggttggtat aaatagacgt tcatgttggt ttttgtttca gttgcaagtt 1800

1741 caagcggtag gccaaccata tttatctgca agtacaacca aaaacaaagt caacgttcaa 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .... PstI(1845),PmlI(1849) ....

1801 ggctgcggcg cgcgcagcac ctttgccggg atctgccggg ctgcagcacg tgttgacaat 1860

1801 ccgacgccgc gcgcgtcgtg gaaacggccc tagacggccc gacgtcgtgc acaactgtta 1860

.......... .......... .......... .......... .......... EM7_pro

.......... .......... .......... .......... .......... ..........

1861 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg 1920

1861 attagtagcc gtatcatata gccgtatcat attatgctgt tccactcctt gatttggtac 1920

.......... .......... EM7_pro .......... .......... ..........

MscI(1921) .......... .......... .......... .......... ..........

1921 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac 1980

1921 cggttcggaa acagagttct tcttaggtgg gagtaacttt ctcgttgccg atgttagttg 1980

.......... .......... blast .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc 2040

1981 tcgtaggggt agagacttct gatgtcgcag cggtcgcgtc gagagagatc gctgccggcg 2040

.......... .......... blast .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg 2100

2041 tagaagtgac cacagttaca tatagtaaaa tgaccccctg gaacacgtct tgagcaccac 2100

.......... .......... blast .......... .......... ..........

.......... ......... PvuII(2125) ......... NruI(2149) ..........

2101 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat 2160

2101 gacccgtgac gacgacgacg ccgtcgaccg ttggactgaa catagcagcg ctagccttta 2160

.......... .......... blast .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gagaacaggg gcatcttgag cccctgcgga cggtgtcgac aggtgcttct cgatctgcat 2220

2161 ctcttgtccc cgtagaactc ggggacgcct gccacagctg tccacgaaga gctagacgta 2220

.......... .......... blast .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cctgggatca aagcgatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt 2280

2221 ggaccctagt ttcgctatca cttcctgtca ctacctgtcg gctgccgtca accctaagca 2280

.......... .......... blast .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg aggagcagga 2340

2281 cttaacgacg ggagaccaat acacaccctc ccgattcgtg aagcaccggc tcctcgtcct 2340

.......... blast .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctgaccgacg ccgaccaaca ccgccggtcc gacgcggccc gacgggtccg aggggggtcg 2400

2341 gactggctgc ggctggttgt ggcggccagg ctgcgccggg ctgcccaggc tccccccagc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acctcgaaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 2460

2401 tggagctttg aacaaataac gtcgaatatt accaatgttt atttcgttat cgtagtgttt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 2520

2461 aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt ttgagtagtt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgtatcttat catgtct... .......... .......... .......... .......... 2537

2521 acatagaata gtacaga... .......... .......... .......... .......... 2537

.......... .......... .......... .......... .......... ..........