ObObiLOCUS pIB/His B 3544 bp DNA SYN 11-Sep-2007 DEFINITION pIB/His B ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3544 /organism="pIB/His B" /mol_type="other DNA" misc_feature 511..530 /label="OpIE2_fwd_primer" misc_feature 578..595 /label="6xHis" misc_feature 598..616 /label="Xpress_fwd_primer" misc_feature 599..631 /label="T7_gene10_leader" misc_feature 599..631 /label="T7_leader" misc_feature 635..658 /label="Xpress_EK" misc_feature 733..750 /label="6xHis" rep_origin complement(1010..1629) /label="pBR322_origin" promoter 2003..2070 /label="EM7_promoter" gene 2071..2469 /label="blast(variant)" /gene="blast(variant)" CDS 2589..3449 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 2589..3449 /label="Ampicillin" /gene="Ampicillin" ORIGIN 1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG 61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT 121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC 181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC 241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC 301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT 361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA 421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC 481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG 541 CATCTGTTCG AATTTAAAGC TTACCATGGG GGGTTCTCAT CATCATCATC ATCATGGTAT 601 GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG TACGACGATG ACGATAAGGT 661 ACCTAAGGAT CCAGTGTGGT GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG 721 TCTAGAGCCG GTCATCATCA CCATCACCAT TGAGTTTATC TGACTAAATC TTAGTTTGTA 781 TTGTCATGTT TTAATACAAT ATGTTATGTT TAAATATGTT TTTAATAAAT TTTATAAAAT 841 AATTTCAACT TTTATTGTAA CAACATTGTC CATTTACACA CTCCTTTCAA GCGCGTGGGA 901 TCGATGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 961 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 1021 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 1081 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 1141 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 1201 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 1261 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 1321 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 1381 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 1441 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 1501 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 1561 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 1621 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 1681 TGGTCATGCG AAACACGCAC GGCGCGCGCA CGCAGCTTAG CACAAACGCG TCGTTGCACG 1741 CGCCCACCGC TAACCGCAGG CCAATCGGTC GGCCGGCCTC ATATCCGCTC ACCAGCCGCG 1801 TCCTATCGGG CGCGGCTTCC GCGCCCATTT TGAATAAATA AACGATAACG CCGTTGGTGG 1861 CGTGAGGCAT GTAAAAGGTT ACATCATTAT CTTGTTCGCC ATCCGGTTGG TATAAATAGA 1921 CGTTCATGTT GGTTTTTGTT TCAGTTGCAA GTTGGCTGCG GCGCGCGCAG CACCTTTGCC 1981 GGGATCTGCC GGGCTGCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA 2041 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGC CTTTGTCTCA AGAAGAATCC 2101 ACCCTCATTG AAAGAGCAAC GGCTACAATC AACAGCATCC CCATCTCTGA AGACTACAGC 2161 GTCGCCAGCG CAGCTCTCTC TAGCGACGGC CGCATCTTCA CTGGTGTCAA TGTATATCAT 2221 TTTACTGGGG GACCTTGCGC AGAACTCGTG GTGCTGGGCA CTGCTGCTGC TGCGGCAGCT 2281 GGCAACCTGA CTTGTATCGT CGCGATCGGA AATGAGAACA GGGGCATCTT GAGCCCCTGC 2341 GGACGGTGCC GACAGGTTCT TCTCGATCTG CATCCTGGGA TCAAAGCCAT AGTGAAGGAC 2401 AGTGATGGAC AGCCGACGGC AGTTGGGATT CGTGAATTGC TGCCCTCTGG TTATGTGTGG 2461 GAGGGCTAAG CACTTCGTGG CCGAGGAGCA GGACTGACAC GTCCCGGGAG ATCTGCATGT 2521 CTACTAAACT CACAAATTAG AGCTTCAATT TAATTATATC AGTTATTACC CATTGAAAAA 2581 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 2641 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 2701 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 2761 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 2821 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 2881 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 2941 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 3001 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 3061 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 3121 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 3181 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 3241 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 3301 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 3361 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 3421 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 3481 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 3541 ATCT //  pIB/His B "Times New RomanObC OpIE2_fwd_pri"Times New RomanObCBS6xHis"Times New RomanObCɆVhXpress_fwd_pri"Times New RomanObbwT7_gene10_leader"Times New RomanObCbw T7_leader"Times New RomanObC{ Xpress_EK"Times New RomanObCɆ6xHis"Times New RomanOb] pBR322_origin"Times New RomanObCEM7_pro"Times New RomanObC blast(variant)"Times New RomanObCɆ y ORF 3"Times New RomanOb y  Ampicillin"Times New Roman ObApaI"Times New RomanVMC4C Ob+NarI(475),BstBI(548),HindIII(557),NheI(602)"Times New Roman$+22N{ ObHKpnI(662),BamHI(667),EcoRI(682),EcoRV(694),NotI(709),XhoI(715),XbaI(721)"Times New Roman h NWll ObClaI"Times New Romanb5l5v5v.< ObPmlI(2002),MscI(2074)"Times New Roman- >M0M0N ObPvuII(2278),NruI(2302)"Times New Roman~ ObSmaI(2505),BglII(2509)"Times New Roman M{ Obtest"Times New Romanl;sGk