Sequence of pIB_His

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Sequence of pIB_His_B

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Sequence of pIB/His B : 3544 BP; 901 A; 883 C; 866 G; 894 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg 60

1 gtactactat ttgttacata ccacgattac aacgaagttg ttgttaagac aacttgacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt 120

61 aaaagtacaa acggttgttc gtggaaatat gagccaccgg aggggtggtg gttgaaaaaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(154) .......... ..........

121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc 180

121 cgtgacgttt ttttgtgcga aaacgtgcgc ccgggtatgt atcatgtttg agatgcaaag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac 240

181 catctgataa aatgtattta tcagatgtgg caacatatgc gaggtttatg tgatggtgtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc 300

241 taacttggaa aaacgtcacg ttttttcatg cacagccgtc agtgcatccg gccggaatag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt 360

301 cccagcgcag gacagtgcat gcttagtgta atagcctggc ctgctcacaa cagaatagca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca 420

361 ctgtcctgcg gtcgaaggac acaacgattg gcgtcggcct gcgttgagga atagccttgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc 480

421 cctgcgcgga ggtatagtcg gcgcgcaata gagtacgcgc actggcctgt gctccgcggg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag 540

481 cagggcgaat agcgcggata tttatgtcgg gcgttgctag accatttgtg tcaacttgtc 540

.......... .......... ........ OpIE2_fwd_pri ........ ..........

BstBI(548) .......... .......... .......... .......... ..........

541 catctgttcg aatttaaagc ttaccatggg gggttctcat catcatcatc atcatggtat 600

541 gtagacaagc ttaaatttcg aatggtaccc cccaagagta gtagtagtag tagtaccata 600

.......... .......... .......... .......... 6xHis ..........

NheI(602) .......... .......... .......... .......... ..........

601 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataaggt 660

601 ccgatcgtac tgaccacctg tcgtttaccc agccctagac atgctgctac tgctattcca 660

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

KpnI2),BamHI(667) EcoRI(682) EcoRV(694) NotI(709) ..........

661 acctaaggat ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag 720

661 tggattccta ggtcacacca ccttaagacg tctataggtc gtgtcaccgc cggcgagctc 720

.......... .......... .......... .......... .......... ..........

XbaI(721) .......... .......... .......... .......... ..........

721 tctagagccg gtcatcatca ccatcaccat tgagtttatc tgactaaatc ttagtttgta 780

721 agatctcggc cagtagtagt ggtagtggta actcaaatag actgatttag aatcaaacat 780

.......... .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttgtcatgtt ttaatacaat atgttatgtt taaatatgtt tttaataaat tttataaaat 840

781 aacagtacaa aattatgtta tacaatacaa atttatacaa aaattattta aaatatttta 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aatttcaact tttattgtaa caacattgtc catttacaca ctcctttcaa gcgcgtggga 900

841 ttaaagttga aaataacatt gttgtaacag gtaaatgtgt gaggaaagtt cgcgcaccct 900

.......... .......... .......... .......... .......... ..........

ClaI(901) .......... .......... .......... .......... ..........

901 tcgatgctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 960

901 agctacgagt gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1020

961 tcttgtacac tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc 1020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1080

1021 gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1140

1081 ccaccgcttt gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1200

1141 acgcgagagg acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1260

1201 cttcgcaccg cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1320

1261 cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1380

1321 cattgatagc agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1440

1381 gaccattgtc ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1500

1441 ccggattgat gccgatgtga tcttcttgtc ataaaccata gacgcgagac gacttcggtc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1560

1501 aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1620

1561 caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1680

1621 gaaactagaa aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa 1680

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tggtcatgcg aaacacgcac ggcgcgcgca cgcagcttag cacaaacgcg tcgttgcacg 1740

1681 accagtacgc tttgtgcgtg ccgcgcgcgt gcgtcgaatc gtgtttgcgc agcaacgtgc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgcccaccgc taaccgcagg ccaatcggtc ggccggcctc atatccgctc accagccgcg 1800

1741 gcgggtggcg attggcgtcc ggttagccag ccggccggag tataggcgag tggtcggcgc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcctatcggg cgcggcttcc gcgcccattt tgaataaata aacgataacg ccgttggtgg 1860

1801 aggatagccc gcgccgaagg cgcgggtaaa acttatttat ttgctattgc ggcaaccacc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgtgaggcat gtaaaaggtt acatcattat cttgttcgcc atccggttgg tataaataga 1920

1861 gcactccgta cattttccaa tgtagtaata gaacaagcgg taggccaacc atatttatct 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgttcatgtt ggtttttgtt tcagttgcaa gttggctgcg gcgcgcgcag cacctttgcc 1980

1921 gcaagtacaa ccaaaaacaa agtcaacgtt caaccgacgc cgcgcgcgtc gtggaaacgg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gggatctgcc gggctgcagc acgtgttgac aattaatcat cggcatagta tatcggcata 2040

1981 ccctagacgg cccgacgtcg tgcacaactg ttaattagta gccgtatcat atagccgtat 2040

.......... .......... .......... .......... EM7_pro ..........

.......... .......... .......... MscI(2074) .......... ..........

2041 gtataatacg acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc 2100

2041 catattatgc tgttccactc cttgatttgg taccggttcg gaaacagagt tcttcttagg 2100

.......... EM7_pro .......... ........ blast(variant) ........

.......... .......... .......... .......... .......... ..........

2101 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc 2160

2101 tgggagtaac tttctcgttg ccgatgttag ttgtcgtagg ggtagagact tctgatgtcg 2160

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat 2220

2161 cagcggtcgc gtcgagagag atcgctgccg gcgtagaagt gaccacagtt acatatagta 2220

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tttactgggg gaccttgcgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct 2280

2221 aaatgacccc ctggaacgcg tcttgagcac cacgacccgt gacgacgacg acgccgtcga 2280

.......... ........ blast(variant) ........ .......... ..........

.......... .......... NruI(2302) .......... .......... ..........

2281 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc 2340

2281 ccgttggact gaacatagca gcgctagcct ttactcttgt ccccgtagaa ctcggggacg 2340

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggacggtgcc gacaggttct tctcgatctg catcctggga tcaaagccat agtgaaggac 2400

2341 cctgccacgg ctgtccaaga agagctagac gtaggaccct agtttcggta tcacttcctg 2400

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg 2460

2401 tcactacctg tcggctgccg tcaaccctaa gcacttaacg acgggagacc aatacacacc 2460

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .... SmaI(2505),BglII(2509) ....

2461 gagggctaag cacttcgtgg ccgaggagca ggactgacac gtcccgggag atctgcatgt 2520

2461 ctcccgattc gtgaagcacc ggctcctcgt cctgactgtg cagggccctc tagacgtaca 2520

blast(variant) ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctactaaact cacaaattag agcttcaatt taattatatc agttattacc cattgaaaaa 2580

2521 gatgatttga gtgtttaatc tcgaagttaa attaatatag tcaataatgg gtaacttttt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 2640

2581 ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa 2640

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 2700

2641 cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 2760

2701 acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 2820

2761 aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc 2820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 2880

2821 ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 2940

2881 tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 3000

2941 ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 3060

3001 gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 3120

3061 gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 3180

3121 ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 3240

3181 gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 3300

3241 aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 3360

3301 cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 3420

3361 aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 3480

3421 atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa 3480

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 3540

3481 tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atct...... .......... .......... .......... .......... .......... 3544

3541 taga...... .......... .......... .......... .......... .......... 3544

.......... .......... .......... .......... .......... ..........