QQgLOCUS pIB/His C 3542 bp DNA SYN 07-Sep-2007 DEFINITION pIB/His C ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3542 /organism="pIB/His C" /mol_type="other DNA" misc_feature 511..530 /label="OpIE2_fwd_primer" misc_feature 578..595 /label="6xHis" misc_feature 598..616 /label="Xpress_fwd_primer" misc_feature 599..631 /label="T7_gene10_leader" misc_feature 599..631 /label="T7_leader" misc_feature 635..658 /label="Xpress_EK" misc_feature 731..748 /label="6xHis" rep_origin complement(1008..1627) /label="pBR322_origin" promoter 2001..2068 /label="EM7_promoter" gene 2069..2467 /label="blast(variant)" /gene="blast(variant)" CDS 2587..3447 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 2587..3447 /label="Ampicillin" /gene="Ampicillin" ORIGIN 1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG 61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT 121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC 181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC 241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC 301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT 361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA 421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCGCG TGACCGGACA CGAGGCGCCC 481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG 541 CATCTGTTCG AATTTAAAGC TTACCATGGG GGGTTCTCAT CATCATCATC ATCATGGTAT 601 GGCTAGCATG ACTGGTGGAC AGCAAATGGG TCGGGATCTG TACGACGATG ACGATAAGGT 661 ACCAGGATCC AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC 721 TAGAGCCGGT CATCATCACC ATCACCATTG AGTTTATCTG ACTAAATCTT AGTTTGTATT 781 GTCATGTTTT AATACAATAT GTTATGTTTA AATATGTTTT TAATAAATTT TATAAAATAA 841 TTTCAACTTT TATTGTAACA ACATTGTCCA TTTACACACT CCTTTCAAGC GCGTGGGATC 901 GATGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG 961 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 1021 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG 1081 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 1141 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA 1201 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC 1261 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT 1321 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT 1381 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 1441 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT 1501 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT 1561 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT 1621 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 1681 GTCATGCGAA ACACGCACGG CGCGCGCACG CAGCTTAGCA CAAACGCGTC GTTGCACGCG 1741 CCCACCGCTA ACCGCAGGCC AATCGGTCGG CCGGCCTCAT ATCCGCTCAC CAGCCGCGTC 1801 CTATCGGGCG CGGCTTCCGC GCCCATTTTG AATAAATAAA CGATAACGCC GTTGGTGGCG 1861 TGAGGCATGT AAAAGGTTAC ATCATTATCT TGTTCGCCAT CCGGTTGGTA TAAATAGACG 1921 TTCATGTTGG TTTTTGTTTC AGTTGCAAGT TGGCTGCGGC GCGCGCAGCA CCTTTGCCGG 1981 GATCTGCCGG GCTGCAGCAC GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT 2041 ATAATACGAC AAGGTGAGGA ACTAAACCAT GGCCAAGCCT TTGTCTCAAG AAGAATCCAC 2101 CCTCATTGAA AGAGCAACGG CTACAATCAA CAGCATCCCC ATCTCTGAAG ACTACAGCGT 2161 CGCCAGCGCA GCTCTCTCTA GCGACGGCCG CATCTTCACT GGTGTCAATG TATATCATTT 2221 TACTGGGGGA CCTTGCGCAG AACTCGTGGT GCTGGGCACT GCTGCTGCTG CGGCAGCTGG 2281 CAACCTGACT TGTATCGTCG CGATCGGAAA TGAGAACAGG GGCATCTTGA GCCCCTGCGG 2341 ACGGTGCCGA CAGGTTCTTC TCGATCTGCA TCCTGGGATC AAAGCCATAG TGAAGGACAG 2401 TGATGGACAG CCGACGGCAG TTGGGATTCG TGAATTGCTG CCCTCTGGTT ATGTGTGGGA 2461 GGGCTAAGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT CCCGGGAGAT CTGCATGTCT 2521 ACTAAACTCA CAAATTAGAG CTTCAATTTA ATTATATCAG TTATTACCCA TTGAAAAAGG 2581 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 2641 CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG 2701 GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 2761 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA 2821 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT 2881 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 2941 GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA 3001 ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 3061 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 3121 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT 3181 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 3241 CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT 3301 GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 3361 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 3421 GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG 3481 ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT 3541 CT //  pIB/His C "Times New RomanQC OpIE2_fwd_pri"Times New RomanQCBS6xHis"Times New RomanQCɆVhXpress_fwd_pri"Times New RomanQWwT7_gene10_leader"Times New RomanQCbw T7_leader"Times New RomanQC{ Xpress_EK"Times New RomanQCɆ6xHis"Times New RomanQ[ pBR322_origin"Times New RomanQCEM7_pro"Times New RomanQC blast(variant)"Times New RomanQCɆ w ORF 1"Times New RomanQ w  Ampicillin"Times New Roman QApaI"Times New RomanXOE6E Q+NarI(475),BstBI(548),HindIII(557),NheI(602)"Times New RomanHOVVP QHKpnI(662),BamHI(665),EcoRI(680),EcoRV(692),NotI(707),XhoI(713),XbaI(719)"Times New Roman : r{ QClaI"Times New Roman221*8 QPmlI(2000),MscI(2072)"Times New RomanQ_k&k&D QPvuII(2276),NruI(2300)"Times New Roman QSmaI(2503),BglII(2507)"Times New Roman q} Qtest"Times New Romanv2RlP66