Sequence of pIB_His

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Sequence of pIB_His_C

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Sequence of pIB/His C : 3542 BP; 900 A; 883 C; 866 G; 893 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg 60

1 gtactactat ttgttacata ccacgattac aacgaagttg ttgttaagac aacttgacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt 120

61 aaaagtacaa acggttgttc gtggaaatat gagccaccgg aggggtggtg gttgaaaaaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(154) .......... ..........

121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc 180

121 cgtgacgttt ttttgtgcga aaacgtgcgc ccgggtatgt atcatgtttg agatgcaaag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac 240

181 catctgataa aatgtattta tcagatgtgg caacatatgc gaggtttatg tgatggtgtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc 300

241 taacttggaa aaacgtcacg ttttttcatg cacagccgtc agtgcatccg gccggaatag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt 360

301 cccagcgcag gacagtgcat gcttagtgta atagcctggc ctgctcacaa cagaatagca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca 420

361 ctgtcctgcg gtcgaaggac acaacgattg gcgtcggcct gcgttgagga atagccttgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc 480

421 cctgcgcgga ggtatagtcg gcgcgcaata gagtacgcgc actggcctgt gctccgcggg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag 540

481 cagggcgaat agcgcggata tttatgtcgg gcgttgctag accatttgtg tcaacttgtc 540

.......... .......... ........ OpIE2_fwd_pri ........ ..........

BstBI(548) .......... .......... .......... .......... ..........

541 catctgttcg aatttaaagc ttaccatggg gggttctcat catcatcatc atcatggtat 600

541 gtagacaagc ttaaatttcg aatggtaccc cccaagagta gtagtagtag tagtaccata 600

.......... .......... .......... .......... 6xHis ..........

NheI(602) .......... .......... .......... .......... ..........

601 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataaggt 660

601 ccgatcgtac tgaccacctg tcgtttaccc agccctagac atgctgctac tgctattcca 660

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

KpnI,BamHI EcoRI EcoRV NotI7) XhoI(713) XbaI(719)

661 accaggatcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc 720

661 tggtcctagg tcacaccacc ttaagacgtc tataggtcgt gtcaccgccg gcgagctcag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tagagccggt catcatcacc atcaccattg agtttatctg actaaatctt agtttgtatt 780

721 atctcggcca gtagtagtgg tagtggtaac tcaaatagac tgatttagaa tcaaacataa 780

.......... 6xHis .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtcatgtttt aatacaatat gttatgttta aatatgtttt taataaattt tataaaataa 840

781 cagtacaaaa ttatgttata caatacaaat ttatacaaaa attatttaaa atattttatt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(899)

841 tttcaacttt tattgtaaca acattgtcca tttacacact cctttcaagc gcgtgggatc 900

841 aaagttgaaa ataacattgt tgtaacaggt aaatgtgtga ggaaagttcg cgcaccctag 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gatgctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 960

901 ctacgagtga gtttccgcca ttatgccaat aggtgtctta gtcccctatt gcgtcctttc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 1020

961 ttgtacactc gttttccggt cgttttccgg tccttggcat ttttccggcg caacgaccgc 1020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 1080

1021 aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt tagctgcgag ttcagtctcc 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 1140

1081 accgctttgg gctgtcctga tatttctatg gtccgcaaag ggggaccttc gagggagcac 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 1200

1141 gcgagaggac aaggctggga cggcgaatgg cctatggaca ggcggaaaga gggaagccct 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 1260

1201 tcgcaccgcg aaagagtatc gagtgcgaca tccatagagt caagccacat ccagcaagcg 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 1320

1261 aggttcgacc cgacacacgt gcttgggggg caagtcgggc tggcgacgcg gaataggcca 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 1380

1321 ttgatagcag aactcaggtt gggccattct gtgctgaata gcggtgaccg tcgtcggtga 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 1440

1381 ccattgtcct aatcgtctcg ctccatacat ccgccacgat gtctcaagaa cttcaccacc 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 1500

1441 ggattgatgc cgatgtgatc ttcttgtcat aaaccataga cgcgagacga cttcggtcaa 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 1560

1501 tggaagcctt tttctcaacc atcgagaact aggccgtttg tttggtggcg accatcgcca 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 1620

1561 ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt tcttctagga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 1680

1621 aactagaaaa gatgccccag actgcgagtc accttgcttt tgagtgcaat tccctaaaac 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtcatgcgaa acacgcacgg cgcgcgcacg cagcttagca caaacgcgtc gttgcacgcg 1740

1681 cagtacgctt tgtgcgtgcc gcgcgcgtgc gtcgaatcgt gtttgcgcag caacgtgcgc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cccaccgcta accgcaggcc aatcggtcgg ccggcctcat atccgctcac cagccgcgtc 1800

1741 gggtggcgat tggcgtccgg ttagccagcc ggccggagta taggcgagtg gtcggcgcag 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctatcgggcg cggcttccgc gcccattttg aataaataaa cgataacgcc gttggtggcg 1860

1801 gatagcccgc gccgaaggcg cgggtaaaac ttatttattt gctattgcgg caaccaccgc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgaggcatgt aaaaggttac atcattatct tgttcgccat ccggttggta taaatagacg 1920

1861 actccgtaca ttttccaatg tagtaataga acaagcggta ggccaaccat atttatctgc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttcatgttgg tttttgtttc agttgcaagt tggctgcggc gcgcgcagca cctttgccgg 1980

1921 aagtacaacc aaaaacaaag tcaacgttca accgacgccg cgcgcgtcgt ggaaacggcc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatctgccgg gctgcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt 2040

1981 ctagacggcc cgacgtcgtg cacaactgtt aattagtagc cgtatcatat agccgtatca 2040

.......... .......... .......... EM7_pro .......... ..........

.......... .......... .......... MscI(2072) .......... ..........

2041 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac 2100

2041 tattatgctg ttccactcct tgatttggta ccggttcgga aacagagttc ttcttaggtg 2100

.......... EM7_pro .......... ........ blast(variant) ........

.......... .......... .......... .......... .......... ..........

2101 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt 2160

2101 ggagtaactt tctcgttgcc gatgttagtt gtcgtagggg tagagacttc tgatgtcgca 2160

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt 2220

2161 gcggtcgcgt cgagagagat cgctgccggc gtagaagtga ccacagttac atatagtaaa 2220

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tactggggga ccttgcgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg 2280

2221 atgaccccct ggaacgcgtc ttgagcacca cgacccgtga cgacgacgac gccgtcgacc 2280

.......... ........ blast(variant) ........ .......... ..........

.......... NruI(2300) .......... .......... .......... ..........

2281 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg 2340

2281 gttggactga acatagcagc gctagccttt actcttgtcc ccgtagaact cggggacgcc 2340

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acggtgccga caggttcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag 2400

2341 tgccacggct gtccaagaag agctagacgt aggaccctag tttcggtatc acttcctgtc 2400

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga 2460

2401 actacctgtc ggctgccgtc aaccctaagc acttaacgac gggagaccaa tacacaccct 2460

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .... SmaI(2503),BglII(2507) ....

2461 gggctaagca cttcgtggcc gaggagcagg actgacacgt cccgggagat ctgcatgtct 2520

2461 cccgattcgt gaagcaccgg ctcctcgtcc tgactgtgca gggccctcta gacgtacaga 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 actaaactca caaattagag cttcaattta attatatcag ttattaccca ttgaaaaagg 2580

2521 tgatttgagt gtttaatctc gaagttaaat taatatagtc aataatgggt aactttttcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 2640

2581 ttctcatact cataagttgt aaaggcacag cgggaataag ggaaaaaacg ccgtaaaacg 2640

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2641 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 2700

2641 gaaggacaaa aacgagtggg tctttgcgac cactttcatt ttctacgact tctagtcaac 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 2760

2701 ccacgtgctc acccaatgta gcttgaccta gagttgtcgc cattctagga actctcaaaa 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 2820

2761 gcggggcttc ttgcaaaagg ttactactcg tgaaaatttc aagacgatac accgcgccat 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 2880

2821 aatagggcat aactgcggcc cgttctcgtt gagccagcgg cgtatgtgat aagagtctta 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 2940

2881 ctgaaccaac tcatgagtgg tcagtgtctt ttcgtagaat gcctaccgta ctgtcattct 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 3000

2941 cttaatacgt cacgacggta ttggtactca ctattgtgac gccggttgaa tgaagactgt 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 3060

3001 tgctagcctc ctggcttcct cgattggcga aaaaacgtgt tgtaccccct agtacattga 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 3120

3061 gcggaactag caacccttgg cctcgactta cttcggtatg gtttgctgct cgcactgtgg 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 3180

3121 tgctacggac atcgttaccg ttgttgcaac gcgtttgata attgaccgct tgatgaatga 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 3240

3181 gatcgaaggg ccgttgttaa ttatctgacc tacctccgcc tatttcaacg tcctggtgaa 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 3300

3241 gacgcgagcc gggaaggccg accgaccaaa taacgactat ttagacctcg gccactcgca 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 3360

3301 cccagagcgc catagtaacg tcgtgacccc ggtctaccat tcgggagggc atagcatcaa 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 3420

3361 tagatgtgct gcccctcagt ccgttgatac ctacttgctt tatctgtcta gcgactctat 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 3480

3421 ccacggagtg actaattcgt aaccattgac agtctggttc aaatgagtat atatgaaatc 3480

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 3540

3481 taactaaatt ttgaagtaaa aattaaattt tcctagatcc acttctagga aaaactatta 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ct........ .......... .......... .......... .......... .......... 3542

3541 ga........ .......... .......... .......... .......... .......... 3542

.......... .......... .......... .......... .......... ..........