\(\("LOCUS pIB/V5-His-DEST 5038 bp DNA SYN 13-Sep-2007 DEFINITION pIB/V5-His-DEST ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5038 /organism="pIB/V5-His-DEST" /mol_type="other DNA" misc_feature 511..530 /label="OpIE2_fwd_primer" misc_feature 602..726 /label="attR1" misc_feature complement(626..726) /label="attR2" promoter 751..774 /label="lac_promoter" CDS 835..1494 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 835..1494 /label="CAT/CamR" /gene="CAT/CamR" misc_feature 1836..2141 /label="ccdB" misc_feature 2182..2282 /label="attR2" misc_feature complement(2182..2289) /label="attR1" misc_feature 2341..2382 /label="V5" misc_feature 2392..2409 /label="6xHis" rep_origin complement(2669..3288) /label="pBR322_origin" promoter 3497..3564 /label="EM7_promoter" gene 3565..3963 /label="blast(variant)" /gene="blast(variant)" CDS 4083..4943 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 4083..4943 /label="Ampicillin" /gene="Ampicillin" ORIGIN 1 CATGATGATA AACAATGTAT GGTGCTAATG TTGCTTCAAC AACAATTCTG TTGAACTGTG 61 TTTTCATGTT TGCCAACAAG CACCTTTATA CTCGGTGGCC TCCCCACCAC CAACTTTTTT 121 GCACTGCAAA AAAACACGCT TTTGCACGCG GGCCCATACA TAGTACAAAC TCTACGTTTC 181 GTAGACTATT TTACATAAAT AGTCTACACC GTTGTATACG CTCCAAATAC ACTACCACAC 241 ATTGAACCTT TTTGCAGTGC AAAAAAGTAC GTGTCGGCAG TCACGTAGGC CGGCCTTATC 301 GGGTCGCGTC CTGTCACGTA CGAATCACAT TATCGGACCG GACGAGTGTT GTCTTATCGT 361 GACAGGACGC CAGCTTCCTG TGTTGCTAAC CGCAGCCGGA CGCAACTCCT TATCGGAACA 421 GGACGCGCCT CCATATCAGC CGCGCGTTAT CTCATGCACG TGACCGGACA CGAGGCGCCC 481 GTCCCGCTTA TCGCGCCTAT AAATACAGCC CGCAACGATC TGGTAAACAC AGTTGAACAG 541 CATCTGTTCG AATTTAAAGC TTGATATCGA ATTCCTGCAG CCCAGCGCTG GATCCTCGAT 601 CACAAGTTTG TACAAAAAAG CTGAACGAGA AACGTAAAAT GATATAAATA TCAATATATT 661 AAATTAGATT TTGCATAAAA AACAGACTAC ATAATACTGT AAAACACAAC ATATCCAGTC 721 ACTATGGCGG CCGCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATAAT 781 GTGTGGATTT TGAGTTAGGA TCCGTCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG 841 AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT 901 GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG 961 GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT 1021 CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG CAATGAAAGA CGGTGAGCTG 1081 GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC ATGAGCAAAC TGAAACGTTT 1141 TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT TTCTACACAT ATATTCGCAA 1201 GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA AAGGGTTTAT TGAGAATATG 1261 TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT TTGATTTAAA CGTGGCCAAT 1321 ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT ATTATACGCA AGGCGACAAG 1381 GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTTT GTGATGGCTT CCATGTCGGC 1441 AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC AGGGCGGGGC GTAAACGCGT 1501 GGATCCGGCT TACTAAAAGC CAGATAACAG TATGCGTATT TGCGCGCTGA TTTTTGCGGT 1561 ATAAGAATAT ATACTGATAT GTATACCCGA AGTATGTCAA AAAGAGGTAT GCTATGAAGC 1621 AGCGTATTAC AGTGACAGTT GACAGCGACA GCTATCAGTT GCTCAAGGCA TATATGATGT 1681 CAATATCTCC GGTCTGGTAA GCACAACCAT GCAGAATGAA GCCCGTCGTC TGCGTGCCGA 1741 ACGCTGGAAA GCGGAAAATC AGGAAGGGAT GGCTGAGGTC GCCCGGTTTA TTGAAATGAA 1801 CGGCTCTTTT GCTGACGAGA ACAGGGGCTG GTGAAATGCA GTTTAAGGTT TACACCTATA 1861 AAAGAGAGAG CCGTTATCGT CTGTTTGTGG ATGTACAGAG TGATATTATT GACACGCCCG 1921 GGCGACGGAT GGTGATCCCC CTGGCCAGTG CACGTCTGCT GTCAGATAAA GTCTCCCGTG 1981 AACTTTACCC GGTGGTGCAT ATCGGGGATG AAAGCTGGCG CATGATGACC ACCGATATGG 2041 CCAGTGTGCC GGTCTCCGTT ATCGGGGAAG AAGTGGCTGA TCTCAGCCAC CGCGAAAATG 2101 ACATCAAAAA CGCCATTAAC CTGATGTTCT GGGGAATATA AATGTCAGGC TCCCTTATAC 2161 ACAGCCAGTC TGCAGGTCGA CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA 2221 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT 2281 CGTTCAGCTT TCTTGTACAA AGTGGTGATC GACCCGGGTC TAGAGGGCCC GCGGTTCGAA 2341 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGCGTACCGG TCATCATCAC 2401 CATCACCATT GAGTTTATCT GACTAAATCT TAGTTTGTAT TGTCATGTTT TAATACAATA 2461 TGTTATGTTT AAATATGTTT TTAATAAATT TTATAAAATA ATTTCAACTT TTATTGTAAC 2521 AACATTGTCC ATTTACACAC TCCTTTCAAG CGCGTGGGAT CGATGCTCAC TCAAAGGCGG 2581 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 2641 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 2701 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC 2761 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC 2821 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA 2881 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 2941 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 3001 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG 3061 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA 3121 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG 3181 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 3241 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 3301 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCCC TTGTTCCGAA 3361 GGGTTGTGTC ACGTAGGCCA GATAACGGTC GGGTATATAA GATGCCTCAA TGCTACTAGT 3421 AAATCAGTCA CACCAAGGCT TCAATAAGGA ACACACAAGC AAGCCCTTTG AGTCAAGGGC 3481 TGCCGGGCTG CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA 3541 TACGACAAGG TGAGGAACTA AACCATGGCC AAGCCTTTGT CTCAAGAAGA ATCCACCCTC 3601 ATTGAAAGAG CAACGGCTAC AATCAACAGC ATCCCCATCT CTGAAGACTA CAGCGTCGCC 3661 GGCGCAGCTC TCTCTAGCGA CGGCCGCATC TTCACTGGTG TCAATGTATA TCATTTTACT 3721 GGGGGACCTT GCGCAGAACT CGTGGTGCTG GGCACTGCTG CTGCTGCGGC AGCTGGCAAC 3781 CTGACTTGTA TCGTCGCGAT CGGAAATGAG AACAGGGGCA TCTTGAGCCC CTGCGGACGG 3841 TGCCGACAGG TTCTTCTCGA TCTGCATCCT GGGATCAAAG CCATAGTGAA GGACAGTGAT 3901 GGACAGCCGA CGGCAGTTGG GATTCGTGAA TTGCTGCCCT CTGGTTATGT GTGGGAGGGC 3961 TAAGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTCCCG GGAGATCTGC ATGTCTACTA 4021 AACTCACAAA TTAGAGCTTC AATTTAATTA TATCAGTTAT TACCCATTGA AAAAGGAAGA 4081 GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC 4141 CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG 4201 CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC 4261 CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT 4321 CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT 4381 TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT 4441 TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA 4501 TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC 4561 TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA 4621 TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG 4681 CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC 4741 GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT 4801 CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT 4861 ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG 4921 CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG 4981 ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCT //pIB/V5-His-DEST "Times New Roman\(C OpIE2_fwd_pri"Times New Roman\(CZattR1"Times New Roman\(CɆrattR2"Times New Roman\(lac_pro"Times New Roman\(CCORF 1"Times New Roman\(CCCAT/CamR"Times New Roman\(CɆ,]ccdB"Times New Roman\(attR2"Times New Roman\(CattR1"Times New Roman\(C% N V5"Times New Roman\(CɆX i 6xHis"Times New Roman\(m  pBR322_origin"Times New Roman\(C EM7_pro"Times New Roman\(C {blast(variant)"Times New Roman\(CɆOORF 3"Times New Roman\(O Ampicillin"Times New Roman \(!NarI(475),HindIII(557),EcoRV(565)"Times New Roman${)r)Err \(NotI"Times New RomanS[bb \(SalI(2176),XbaI(2319)"Times New Roman (0(0kN \(SacII(2332),AgeI(2386)"Times New Roman G"8OOm \(ClaI"Times New Roman '1:o:I \(SpeI"Times New RomanW { \(NruI"Times New Roman554f-; \(BglII"Times New Romano \(test"Times New RomanC. \(test"Times New RomanG %T"