Sequence of pIB_V5-His-DEST

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Sequence of pIB_V5-His-DEST

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Sequence of pIB/V5-His-DEST : 5038 BP; 1342 A; 1178 C; 1198 G; 1320 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg 60

1 gtactactat ttgttacata ccacgattac aacgaagttg ttgttaagac aacttgacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt 120

61 aaaagtacaa acggttgttc gtggaaatat gagccaccgg aggggtggtg gttgaaaaaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc 180

121 cgtgacgttt ttttgtgcga aaacgtgcgc ccgggtatgt atcatgtttg agatgcaaag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac 240

181 catctgataa aatgtattta tcagatgtgg caacatatgc gaggtttatg tgatggtgtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc 300

241 taacttggaa aaacgtcacg ttttttcatg cacagccgtc agtgcatccg gccggaatag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt 360

301 cccagcgcag gacagtgcat gcttagtgta atagcctggc ctgctcacaa cagaatagca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca 420

361 ctgtcctgcg gtcgaaggac acaacgattg gcgtcggcct gcgttgagga atagccttgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcacg tgaccggaca cgaggcgccc 480

421 cctgcgcgga ggtatagtcg gcgcgcaata gagtacgtgc actggcctgt gctccgcggg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag 540

481 cagggcgaat agcgcggata tttatgtcgg gcgttgctag accatttgtg tcaacttgtc 540

.......... .......... ........ OpIE2_fwd_pri ........ ..........

......... HindIII(557) EcoRV(565) ......... .......... ..........

541 catctgttcg aatttaaagc ttgatatcga attcctgcag cccagcgctg gatcctcgat 600

541 gtagacaagc ttaaatttcg aactatagct taaggacgtc gggtcgcgac ctaggagcta 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 660

601 gtgttcaaac atgttttttc gacttgctct ttgcatttta ctatatttat agttatataa 660

.......... .......... .......... attR1 attR2 ..........

.......... .......... .......... .......... .......... ..........

661 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 720

661 tttaatctaa aacgtatttt ttgtctgatg tattatgaca ttttgtgttg tataggtcag 720

.......... ......... attR1 attR2 ......... .......... ..........

NotI(728) .......... .......... .......... .......... ..........

721 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 780

721 tgataccgcc ggcgtaatcc gtggggtccg aaatgtgaaa tacgaaggcc gagcatatta 780

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

781 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag 840

781 cacacctaaa actcaatcct aggcagctct aaaagtcctc gattccttcg attttacctc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 900

841 tttttttagt gacctatatg gtggcaacta tatagggtta ccgtagcatt tcttgtaaaa 900

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 960

901 ctccgtaaag tcagtcaacg agttacatgg atattggtct ggcaagtcga cctataatgc 960

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 1020

961 cggaaaaatt tctggcattt ctttttattc gtgttcaaaa taggccggaa ataagtgtaa 1020

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 1080

1021 gaacgggcgg actacttacg agtaggcctt aaggcatacc gttactttct gccactcgac 1080

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 1140

1081 cactataccc tatcacaagt gggaacaatg tggcaaaagg tactcgtttg actttgcaaa 1140

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 1200

1141 agtagcgaga cctcacttat ggtgctgcta aaggccgtca aagatgtgta tataagcgtt 1200

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 1260

1201 ctacaccgca caatgccact tttggaccgg ataaagggat ttcccaaata actcttatac 1260

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 1320

1261 aaaaagcaga gtcggttagg gacccactca aagtggtcaa aactaaattt gcaccggtta 1320

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 1380

1321 tacctgttga agaagcgggg gcaaaagtgg tacccgttta taatatgcgt tccgctgttc 1380

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc 1440

1381 cacgactacg gcgaccgcta agtccaagta gtacggcaaa cactaccgaa ggtacagccg 1440

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt 1500

1441 tcttacgaat tacttaatgt tgtcatgacg ctactcaccg tcccgccccg catttgcgca 1500

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 1560

1501 cctaggccga atgattttcg gtctattgtc atacgcataa acgcgcgact aaaaacgcca 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc 1620

1561 tattcttata tatgactata catatgggct tcatacagtt tttctccata cgatacttcg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 1680

1621 tcgcataatg tcactgtcaa ctgtcgctgt cgatagtcaa cgagttccgt atatactaca 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 1740

1681 gttatagagg ccagaccatt cgtgttggta cgtcttactt cgggcagcag acgcacggct 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 1800

1741 tgcgaccttt cgccttttag tccttcccta ccgactccag cgggccaaat aactttactt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata 1860

1801 gccgagaaaa cgactgctct tgtccccgac cactttacgt caaattccaa atgtggatat 1860

.......... .......... .......... .......... ccdB ..........

.......... .......... .......... .......... .......... ..........

1861 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 1920

1861 tttctctctc ggcaatagca gacaaacacc tacatgtctc actataataa ctgtgcgggc 1920

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 1980

1921 ccgctgccta ccactagggg gaccggtcac gtgcagacga cagtctattt cagagggcac 1980

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 2040

1981 ttgaaatggg ccaccacgta tagcccctac tttcgaccgc gtactactgg tggctatacc 2040

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 2100

2041 ggtcacacgg ccagaggcaa tagccccttc ttcaccgact agagtcggtg gcgcttttac 2100

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac 2160

2101 tgtagttttt gcggtaattg gactacaaga ccccttatat ttacagtccg agggaatatg 2160

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 2220

2161 tgtcggtcag acgtccagct ggtatcactg acctatacaa cacaaaatgt cataatacat 2220

.......... .......... .......... ......... attR2 attR1 .........

.......... .......... .......... .......... .......... ..........

2221 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 2280

2221 cagacaaaaa atacgtttta gattaaatta tataactata aatatagtaa aatgcaaaga 2280

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... XbaI(2319) .......... ..........

2281 cgttcagctt tcttgtacaa agtggtgatc gacccgggtc tagagggccc gcggttcgaa 2340

2281 gcaagtcgaa agaacatgtt tcaccactag ctgggcccag atctcccggg cgccaagctt 2340

attR1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(2386) ..........

2341 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac 2400

2341 ccattcggat agggattggg agaggagcca gagctaagat gcgcatggcc agtagtagtg 2400

.......... .......... V5 .......... .......... 6xHis

.......... .......... .......... .......... .......... ..........

2401 catcaccatt gagtttatct gactaaatct tagtttgtat tgtcatgttt taatacaata 2460

2401 gtagtggtaa ctcaaataga ctgatttaga atcaaacata acagtacaaa attatgttat 2460

6xHis .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgttatgttt aaatatgttt ttaataaatt ttataaaata atttcaactt ttattgtaac 2520

2461 acaatacaaa tttatacaaa aattatttaa aatattttat taaagttgaa aataacattg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ClaI(2560) .......... ..........

2521 aacattgtcc atttacacac tcctttcaag cgcgtgggat cgatgctcac tcaaaggcgg 2580

2521 ttgtaacagg taaatgtgtg aggaaagttc gcgcacccta gctacgagtg agtttccgcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 2640

2581 attatgccaa taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 2700

2641 tcgttttccg gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg 2700

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2701 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 2760

2701 ggggactgct cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 2820

2761 atatttctat ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 2880

2821 acggcgaatg gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 2940

2881 cgagtgcgac atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 3000

2941 tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 3060

3001 tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 3120

3061 gctccataca tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 3180

3121 cttcttgtca taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 3240

3181 catcgagaac taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 3300

3241 tcgtctaatg cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgccc ttgttccgaa 3360

3301 gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa ccagtacggg aacaaggctt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SpeI(3415)

3361 gggttgtgtc acgtaggcca gataacggtc gggtatataa gatgcctcaa tgctactagt 3420

3361 cccaacacag tgcatccggt ctattgccag cccatatatt ctacggagtt acgatgatca 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaatcagtca caccaaggct tcaataagga acacacaagc aagccctttg agtcaagggc 3480

3421 tttagtcagt gtggttccga agttattcct tgtgtgttcg ttcgggaaac tcagttcccg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgccgggctg cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa 3540

3481 acggcccgac gtcgtgcaca actgttaatt agtagccgta tcatatagcc gtatcatatt 3540

.......... .......... .......... EM7_pro .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc 3600

3541 atgctgttcc actccttgat ttggtaccgg ttcggaaaca gagttcttct taggtgggag 3600

.......... EM7_pro .......... ........ blast(variant) ........

.......... .......... .......... .......... .......... ..........

3601 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc 3660

3601 taactttctc gttgccgatg ttagttgtcg taggggtaga gacttctgat gtcgcagcgg 3660

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact 3720

3661 ccgcgtcgag agagatcgct gccggcgtag aagtgaccac agttacatat agtaaaatga 3720

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gggggacctt gcgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac 3780

3721 ccccctggaa cgcgtcttga gcaccacgac ccgtgacgac gacgacgccg tcgaccgttg 3780

.......... ........ blast(variant) ........ .......... ..........

.......... NruI(3796) .......... .......... .......... ..........

3781 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg 3840

3781 gactgaacat agcagcgcta gcctttactc ttgtccccgt agaactcggg gacgcctgcc 3840

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgccgacagg ttcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat 3900

3841 acggctgtcc aagaagagct agacgtagga ccctagtttc ggtatcactt cctgtcacta 3900

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc 3960

3901 cctgtcggct gccgtcaacc ctaagcactt aacgacggga gaccaataca caccctcccg 3960

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... ......... BglII(4003) .........

3961 taagcacttc gtggccgagg agcaggactg acacgtcccg ggagatctgc atgtctacta 4020

3961 attcgtgaag caccggctcc tcgtcctgac tgtgcagggc cctctagacg tacagatgat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aactcacaaa ttagagcttc aatttaatta tatcagttat tacccattga aaaaggaaga 4080

4021 ttgagtgttt aatctcgaag ttaaattaat atagtcaata atgggtaact ttttccttct 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 4140

4081 catactcata agttgtaaag gcacagcggg aataagggaa aaaacgccgt aaaacggaag 4140

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 4200

4141 gacaaaaacg agtgggtctt tgcgaccact ttcattttct acgacttcta gtcaacccac 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 4260

4201 gtgctcaccc aatgtagctt gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 4320

4261 ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga cgatacaccg cgccataata 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 4380

4321 gggcataact gcggcccgtt ctcgttgagc cagcggcgta tgtgataaga gtcttactga 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 4440

4381 accaactcat gagtggtcag tgtcttttcg tagaatgcct accgtactgt cattctctta 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 4500

4441 atacgtcacg acggtattgg tactcactat tgtgacgccg gttgaatgaa gactgttgct 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 4560

4501 agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta ccccctagta cattgagcgg 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 4620

4561 aactagcaac ccttggcctc gacttacttc ggtatggttt gctgctcgca ctgtggtgct 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 4680

4621 acggacatcg ttaccgttgt tgcaacgcgt ttgataattg accgcttgat gaatgagatc 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 4740

4681 gaagggccgt tgttaattat ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 4800

4741 cgagccggga aggccgaccg accaaataac gactatttag acctcggcca ctcgcaccca 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 4860

4801 gagcgccata gtaacgtcgt gaccccggtc taccattcgg gagggcatag catcaataga 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 4920

4861 tgtgctgccc ctcagtccgt tgatacctac ttgctttatc tgtctagcga ctctatccac 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 4980

4921 ggagtgacta attcgtaacc attgacagtc tggttcaaat gagtatatat gaaatctaac 4980

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatct.. 5038

4981 taaattttga agtaaaaatt aaattttcct agatccactt ctaggaaaaa ctattaga.. 5038

.......... .......... .......... .......... .......... ..........