Sequence of pIB_V5-His-TOPO

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Sequence of pIB_V5-His-TOPO

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Sequence of pIB/V5-His-TOPO : 3537 BP; 891 A; 895 C; 859 G; 892 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg 60

1 gtactactat ttgttacata ccacgattac aacgaagttg ttgttaagac aacttgacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt 120

61 aaaagtacaa acggttgttc gtggaaatat gagccaccgg aggggtggtg gttgaaaaaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc 180

121 cgtgacgttt ttttgtgcga aaacgtgcgc ccgggtatgt atcatgtttg agatgcaaag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac 240

181 catctgataa aatgtattta tcagatgtgg caacatatgc gaggtttatg tgatggtgtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc 300

241 taacttggaa aaacgtcacg ttttttcatg cacagccgtc agtgcatccg gccggaatag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt 360

301 cccagcgcag gacagtgcat gcttagtgta atagcctggc ctgctcacaa cagaatagca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca 420

361 ctgtcctgcg gtcgaaggac acaacgattg gcgtcggcct gcgttgagga atagccttgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc 480

421 cctgcgcgga ggtatagtcg gcgcgcaata gagtacgcgc actggcctgt gctccgcggg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag 540

481 cagggcgaat agcgcggata tttatgtcgg gcgttgctag accatttgtg tcaacttgtc 540

.......... .......... ........ OpIE2_fwd_pri ........ ..........

.... HindIII(557) KpnI(567) SacI(573),BamHI(575) .... ..........

541 catctgttcg aatttaaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa 600

541 gtagacaagc ttaaatttcg aaccatggct cgagcctagg tgatcaggtc acaccacctt 600

.......... .......... .......... .......... .......... ..........

AflII(606) .......... EcoRV(626) ..... NotI(641) XhoI(647) .....

601 ttgcccttaa gggcaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg 660

601 aacgggaatt cccgttaaga cgtctatagg tcgtgtcacc gccggcgagc tcagatctcc 660

.......... .......... .......... .......... .......... ..........

SacII(666) .......... .......... .......... .......... AgeI(720)

661 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta 720

661 cgggcgccaa gcttccattc ggatagggat tgggagagga gccagagcta agatgcgcat 720

.......... .......... .......... V5 .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggtcatca tcaccatcac cattgagttt atctgactaa atcttagttt gtattgtcat 780

721 ggccagtagt agtggtagtg gtaactcaaa tagactgatt tagaatcaaa cataacagta 780

.......... 6xHis .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gttttaatac aatatgttat gtttaaatat gtttttaata aattttataa aataatttca 840

781 caaaattatg ttatacaata caaatttata caaaaattat ttaaaatatt ttattaaagt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(894)

841 acttttattg taacaacatt gtccatttac acactccttt caagcgcgtg ggatcgatgc 900

841 tgaaaataac attgttgtaa caggtaaatg tgtgaggaaa gttcgcgcac cctagctacg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 960

901 agtgagtttc cgccattatg ccaataggtg tcttagtccc ctattgcgtc ctttcttgta 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 1020

961 cactcgtttt ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa 1020

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 1080

1021 ggtatccgag gcggggggac tgctcgtagt gtttttagct gcgagttcag tctccaccgc 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1140

1081 tttgggctgt cctgatattt ctatggtccg caaaggggga ccttcgaggg agcacgcgag 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1200

1141 aggacaaggc tgggacggcg aatggcctat ggacaggcgg aaagagggaa gcccttcgca 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 1260

1201 ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc cacatccagc aagcgaggtt 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 1320

1261 cgacccgaca cacgtgcttg gggggcaagt cgggctggcg acgcggaata ggccattgat 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 1380

1321 agcagaactc aggttgggcc attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 1440

1381 gtcctaatcg tctcgctcca tacatccgcc acgatgtctc aagaacttca ccaccggatt 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 1500

1441 gatgccgatg tgatcttctt gtcataaacc atagacgcga gacgacttcg gtcaatggaa 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 1560

1501 gcctttttct caaccatcga gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 1620

1561 aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc taggaaacta 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 1680

1621 gaaaagatgc cccagactgc gagtcacctt gcttttgagt gcaattccct aaaaccagta 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcgaaacacg cacggcgcgc gcacgcagct tagcacaaac gcgtcgttgc acgcgcccac 1740

1681 cgctttgtgc gtgccgcgcg cgtgcgtcga atcgtgtttg cgcagcaacg tgcgcgggtg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgctaaccgc aggccaatcg gtcggccggc ctcatatccg ctcaccagcc gcgtcctatc 1800

1741 gcgattggcg tccggttagc cagccggccg gagtataggc gagtggtcgg cgcaggatag 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggcgcggct tccgcgccca ttttgaataa ataaacgata acgccgttgg tggcgtgagg 1860

1801 cccgcgccga aggcgcgggt aaaacttatt tatttgctat tgcggcaacc accgcactcc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 catgtaaaag gttacatcat tatcttgttc gccatccggt tggtataaat agacgttcat 1920

1861 gtacattttc caatgtagta atagaacaag cggtaggcca accatattta tctgcaagta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gttggttttt gtttcagttg caagttggct gcggcgcgcg cagcaccttt gccgggatct 1980

1921 caaccaaaaa caaagtcaac gttcaaccga cgccgcgcgc gtcgtggaaa cggccctaga 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gccgggctgc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 2040

1981 cggcccgacg tcgtgcacaa ctgttaatta gtagccgtat catatagccg tatcatatta 2040

.......... .......... .......... EM7_pro .......... ..........

.......... .... NcoI(2062),MscI(2067) .... .......... ..........

2041 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca 2100

2041 tgctgttcca ctccttgatt tggtaccggt tcggaaacag agttcttctt aggtgggagt 2100

.......... EM7_pro .......... ........ blast(variant) ........

.......... .......... .......... .......... .......... ..........

2101 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca 2160

2101 aactttctcg ttgccgatgt tagttgtcgt aggggtagag acttctgatg tcgcagcggt 2160

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg 2220

2161 cgcgtcgaga gagatcgctg ccggcgtaga agtgaccaca gttacatata gtaaaatgac 2220

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggggaccttg cgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc 2280

2221 cccctggaac gcgtcttgag caccacgacc cgtgacgacg acgacgccgt cgaccgttgg 2280

.......... ........ blast(variant) ........ .......... ..........

.......... NruI(2295) .......... .......... .......... ..........

2281 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt 2340

2281 actgaacata gcagcgctag cctttactct tgtccccgta gaactcgggg acgcctgcca 2340

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gccgacaggt tcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg 2400

2341 cggctgtcca agaagagcta gacgtaggac cctagtttcg gtatcacttc ctgtcactac 2400

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct 2460

2401 ctgtcggctg ccgtcaaccc taagcactta acgacgggag accaatacac accctcccga 2460

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .... SmaI(2498),BglII(2502) ....

2461 aagcacttcg tggccgagga gcaggactga cacgtcccgg gagatctgca tgtctactaa 2520

2461 ttcgtgaagc accggctcct cgtcctgact gtgcagggcc ctctagacgt acagatgatt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 actcacaaat tagagcttca atttaattat atcagttatt acccattgaa aaaggaagag 2580

2521 tgagtgttta atctcgaagt taaattaata tagtcaataa tgggtaactt tttccttctc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 2640

2581 atactcataa gttgtaaagg cacagcggga ataagggaaa aaacgccgta aaacggaagg 2640

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2641 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 2700

2641 acaaaaacga gtgggtcttt gcgaccactt tcattttcta cgacttctag tcaacccacg 2700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 2760

2701 tgctcaccca atgtagcttg acctagagtt gtcgccattc taggaactct caaaagcggg 2760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 2820

2761 gcttcttgca aaaggttact actcgtgaaa atttcaagac gatacaccgc gccataatag 2820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 2880

2821 ggcataactg cggcccgttc tcgttgagcc agcggcgtat gtgataagag tcttactgaa 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 2940

2881 ccaactcatg agtggtcagt gtcttttcgt agaatgccta ccgtactgtc attctcttaa 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 3000

2941 tacgtcacga cggtattggt actcactatt gtgacgccgg ttgaatgaag actgttgcta 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 3060

3001 gcctcctggc ttcctcgatt ggcgaaaaaa cgtgttgtac cccctagtac attgagcgga 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 3120

3061 actagcaacc cttggcctcg acttacttcg gtatggtttg ctgctcgcac tgtggtgcta 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 3180

3121 cggacatcgt taccgttgtt gcaacgcgtt tgataattga ccgcttgatg aatgagatcg 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 3240

3181 aagggccgtt gttaattatc tgacctacct ccgcctattt caacgtcctg gtgaagacgc 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 3300

3241 gagccgggaa ggccgaccga ccaaataacg actatttaga cctcggccac tcgcacccag 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 3360

3301 agcgccatag taacgtcgtg accccggtct accattcggg agggcatagc atcaatagat 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 3420

3361 gtgctgcccc tcagtccgtt gatacctact tgctttatct gtctagcgac tctatccacg 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 3480

3421 gagtgactaa ttcgtaacca ttgacagtct ggttcaaatg agtatatatg aaatctaact 3480

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatct... 3537

3481 aaattttgaa gtaaaaatta aattttccta gatccacttc taggaaaaac tattaga... 3537

.......... .......... .......... .......... .......... ..........