Sequence of pIB

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Sequence of pIB_V5-His

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Sequence of pIB/V5-His : 3521 BP; 887 A; 891 C; 855 G; 888 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgata aacaatgtat ggtgctaatg ttgcttcaac aacaattctg ttgaactgtg 60

1 gtactactat ttgttacata ccacgattac aacgaagttg ttgttaagac aacttgacac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttttcatgtt tgccaacaag cacctttata ctcggtggcc tccccaccac caactttttt 120

61 aaaagtacaa acggttgttc gtggaaatat gagccaccgg aggggtggtg gttgaaaaaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcactgcaaa aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc 180

121 cgtgacgttt ttttgtgcga aaacgtgcgc ccgggtatgt atcatgtttg agatgcaaag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gtagactatt ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac 240

181 catctgataa aatgtattta tcagatgtgg caacatatgc gaggtttatg tgatggtgtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 attgaacctt tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc 300

241 taacttggaa aaacgtcacg ttttttcatg cacagccgtc agtgcatccg gccggaatag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gggtcgcgtc ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt 360

301 cccagcgcag gacagtgcat gcttagtgta atagcctggc ctgctcacaa cagaatagca 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gacaggacgc cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca 420

361 ctgtcctgcg gtcgaaggac acaacgattg gcgtcggcct gcgttgagga atagccttgt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggacgcgcct ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc 480

421 cctgcgcgga ggtatagtcg gcgcgcaata gagtacgcgc actggcctgt gctccgcggg 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtcccgctta tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag 540

481 cagggcgaat agcgcggata tttatgtcgg gcgttgctag accatttgtg tcaacttgtc 540

.......... .......... ........ OpIE2_fwd_pri ........ ..........

.... HindIII) KpnI(567) SacI(573),BamHI(575) SpeI(581) ....

541 catctgttcg aatttaaagc ttggtaccga gctcggatcc actagtccag tgtggtggaa 600

541 gtagacaagc ttaaatttcg aaccatggct cgagcctagg tgatcaggtc acaccacctt 600

.......... .......... .......... .......... .......... ..........

EcoRV(610) ..... NotI(625) XhoI(631) XbaI(637) ..... ..........

601 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg 660

601 aagacgtcta taggtcgtgt caccgccggc gagctcagat ctcccgggcg ccaagcttcc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AgeI(704) ..........

661 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca 720

661 attcggatag ggattgggag aggagccaga gctaagatgc gcatggccag tagtagtggt 720

.......... V5 .......... .......... .......... 6xHis

.......... .......... .......... .......... .......... ..........

721 tcaccattga gtttatctga ctaaatctta gtttgtattg tcatgtttta atacaatatg 780

721 agtggtaact caaatagact gatttagaat caaacataac agtacaaaat tatgttatac 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttatgtttaa atatgttttt aataaatttt ataaaataat ttcaactttt attgtaacaa 840

781 aatacaaatt tatacaaaaa ttatttaaaa tattttatta aagttgaaaa taacattgtt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ClaI(878) .......... ..........

841 cattgtccat ttacacactc ctttcaagcg cgtgggatcg atgctcactc aaaggcggta 900

841 gtaacaggta aatgtgtgag gaaagttcgc gcaccctagc tacgagtgag tttccgccat 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 960

901 tatgccaata ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 1020

961 gttttccggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg 1020

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1021 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 1080

1021 ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 1140

1081 atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 1200

1141 ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 1260

1201 agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 1320

1261 cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1380

1321 ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 1440

1381 tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 1500

1441 tcttgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 1560

1501 tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 1620

1561 gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcgaaa cacgcacggc 1680

1621 ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtacgcttt gtgcgtgccg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcgcgcacgc agcttagcac aaacgcgtcg ttgcacgcgc ccaccgctaa ccgcaggcca 1740

1681 cgcgcgtgcg tcgaatcgtg tttgcgcagc aacgtgcgcg ggtggcgatt ggcgtccggt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 atcggtcggc cggcctcata tccgctcacc agccgcgtcc tatcgggcgc ggcttccgcg 1800

1741 tagccagccg gccggagtat aggcgagtgg tcggcgcagg atagcccgcg ccgaaggcgc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cccattttga ataaataaac gataacgccg ttggtggcgt gaggcatgta aaaggttaca 1860

1801 gggtaaaact tatttatttg ctattgcggc aaccaccgca ctccgtacat tttccaatgt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcattatctt gttcgccatc cggttggtat aaatagacgt tcatgttggt ttttgtttca 1920

1861 agtaatagaa caagcggtag gccaaccata tttatctgca agtacaacca aaaacaaagt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gttgcaagtt ggctgcggcg cgcgcagcac ctttgccggg atctgccggg ctgcagcacg 1980

1921 caacgttcaa ccgacgccgc gcgcgtcgtg gaaacggccc tagacggccc gacgtcgtgc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa 2040

1981 acaactgtta attagtagcc gtatcatata gccgtatcat attatgctgt tccactcctt 2040

.......... .......... EM7_pro .......... .......... ..........

.... NcoI(2046),MscI(2051) .... .......... .......... ..........

2041 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc 2100

2041 gatttggtac cggttcggaa acagagttct tcttaggtgg gagtaacttt ctcgttgccg 2100

.......... .......... ........ blast(variant) ........ ..........

.......... .......... .......... .......... .......... ..........

2101 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag 2160

2101 atgttagttg tcgtaggggt agagacttct gatgtcgcag cggtcgcgtc gagagagatc 2160

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgcgcaga 2220

2161 gctgccggcg tagaagtgac cacagttaca tatagtaaaa tgaccccctg gaacgcgtct 2220

.......... ........ blast(variant) ........ .......... ..........

.......... .......... ......... PvuII(2255) ......... NruI(2279)

2221 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc 2280

2221 tgagcaccac gacccgtgac gacgacgacg ccgtcgaccg ttggactgaa catagcagcg 2280

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggttcttct 2340

2281 ctagccttta ctcttgtccc cgtagaactc ggggacgcct gccacggctg tccaagaaga 2340

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt 2400

2341 gctagacgta ggaccctagt ttcggtatca cttcctgtca ctacctgtcg gctgccgtca 2400

.......... ........ blast(variant) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg 2460

2401 accctaagca cttaacgacg ggagaccaat acacaccctc ccgattcgtg aagcaccggc 2460

.......... ........ blast(variant) ........ .......... ..........

.......... .... SmaI(2482),BglII(2486) .... .......... ..........

2461 aggagcagga ctgacacgtc ccgggagatc tgcatgtcta ctaaactcac aaattagagc 2520

2461 tcctcgtcct gactgtgcag ggccctctag acgtacagat gatttgagtg tttaatctcg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttcaatttaa ttatatcagt tattacccat tgaaaaagga agagtatgag tattcaacat 2580

2521 aagttaaatt aatatagtca ataatgggta actttttcct tctcatactc ataagttgta 2580

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

2581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 2640

2581 aaggcacagc gggaataagg gaaaaaacgc cgtaaaacgg aaggacaaaa acgagtgggt 2640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 2700

2641 ctttgcgacc actttcattt tctacgactt ctagtcaacc cacgtgctca cccaatgtag 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca 2760

2701 cttgacctag agttgtcgcc attctaggaa ctctcaaaag cggggcttct tgcaaaaggt 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg 2820

2761 tactactcgt gaaaatttca agacgataca ccgcgccata atagggcata actgcggccc 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 2880

2821 gttctcgttg agccagcggc gtatgtgata agagtcttac tgaaccaact catgagtggt 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 2940

2881 cagtgtcttt tcgtagaatg cctaccgtac tgtcattctc ttaatacgtc acgacggtat 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 3000

2941 tggtactcac tattgtgacg ccggttgaat gaagactgtt gctagcctcc tggcttcctc 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 3060

3001 gattggcgaa aaaacgtgtt gtacccccta gtacattgag cggaactagc aacccttggc 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 3120

3061 ctcgacttac ttcggtatgg tttgctgctc gcactgtggt gctacggaca tcgttaccgt 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 3180

3121 tgttgcaacg cgtttgataa ttgaccgctt gatgaatgag atcgaagggc cgttgttaat 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 3240

3181 tatctgacct acctccgcct atttcaacgt cctggtgaag acgcgagccg ggaaggccga 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 3300

3241 ccgaccaaat aacgactatt tagacctcgg ccactcgcac ccagagcgcc atagtaacgt 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 3360

3301 cgtgaccccg gtctaccatt cgggagggca tagcatcaat agatgtgctg cccctcagtc 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 3420

3361 cgttgatacc tacttgcttt atctgtctag cgactctatc cacggagtga ctaattcgta 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt 3480

3421 accattgaca gtctggttca aatgagtata tatgaaatct aactaaattt tgaagtaaaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taatttaaaa ggatctaggt gaagatcctt tttgataatc t......... .......... 3521

3481 attaaatttt cctagatcca cttctaggaa aaactattag a......... .......... 3521

.......... .......... .......... .......... .......... ..........