Sequence of pIRES2-DsRed-Express

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Sequence of pIRES2-DsRed-Express

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Sequence of pIRES2-DsRed-Express : 5264 BP; 1241 A; 1422 C; 1395 G; 1206 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gt aggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro ...... CMV_fwd_pri .........

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI EcoRI SalI SacII SmaI),BamHI(660)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 720

661 ctaggcgggg agagggaggg gggggggatt gcaatgaccg gcttcggcga accttattcc 720

.......... .......... .......... IRES .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 780

721 ggccacacgc aaacagatat acaataaaag gtggtataac ggcagaaaac cgttacactc 780

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 840

781 ccgggccttt ggaccgggac agaagaactg ctcgtaagga tccccagaaa ggggagagcg 840

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 900

841 gtttccttac gttccagaca acttacagca cttccttcgt caaggagacc ttcgaagaac 900

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 960

901 ttctgtttgt tgcagacatc gctgggaaac gtccgtcgcc ttggggggtg gaccgctgtc 960

.......... .......... IRES .......... .......... ..........

.......... .......... PmlI(983) .......... .......... ..........

961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 1020

961 cacggagacg ccggttttcg gtgcacatat tctatgtgga cgtttccgcc gtgttggggt 1020

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 1080

1021 cacggtgcaa cactcaacct atcaacacct ttctcagttt accgagagga gttcgcataa 1080

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 1140

1081 gttgttcccc gacttcctac gggtcttcca tggggtaaca taccctagac tagaccccgg 1140

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 1200

1141 agccacgtgt acgaaatgta cacaaatcag ctccaatttt tttgcagatc cggggggctt 1200

.......... .......... IRES ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggcct 1260

1201 ggtgcccctg caccaaaagg aaactttttg tgctactatt ataccggtgt tggtaccgga 1260

.......... .......... IRES ORF 3 .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1261 cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga 1320

1261 ggaggctcct gcagtagttc ctcaagtacg cgaagttcca cgcgtacctc ccgaggcact 1320

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 1380

1321 tgccggtgct caagctctag ctcccgctcc cgctcccggc ggggatgctc ccgtgggtct 1380

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc 1440

1381 ggcggttcga cttccactgg ttcccgccgg gggacgggaa gcggaccctg taggacaggg 1440

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc cccgactaca 1500

1441 gggtcaaggt catgccgagg ttccacatgc acttcgtggg gcggctgtag gggctgatgt 1500

dsRed1_N_pri ORF 3 ORF 3 DsRed2(variant) .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 1560

1501 tcttcgacag gaaggggctc ccgaagttca ccctcgcgca ctacttgaag ctcctgccgc 1560

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctccttcatc tacaaggtga 1620

1561 cgcaccactg gcactgggtc ctgaggaggg acgtcctgcc gaggaagtag atgttccact 1620

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag actatgggct 1680

1621 tcaagtagcc gcacttgaag gggaggctgc cggggcatta cgtcttcttc tgatacccga 1680

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccaca 1740

1681 ccctccggag gtggctcgcg gacatggggg cgctgccgca cgacttcccg ctctaggtgt 1740

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtct atctatatgg 1800

1741 tccgggactt cgacttcctg ccgccggtga tggaccacct caagttcaga tagatatacc 1800

.......... . ORF 3 ORF 3 DsRed2(variant) . .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg gacatcacct 1860

1801 ggttcttcgg gcacgtcgac gggccgatga tgatgcacct gaggttcgac ctgtagtgga 1860

.......... . ORF 3 ORF 3 DsRed2(variant) . ......... dsRed1_C_pri

.......... .......... .......... .......... .......... ..........

1861 cccacaacga ggactacacc atcgtggagc agtacgagcg cgccgagggc cgccaccacc 1920

1861 gggtgttgct cctgatgtgg tagcacctcg tcatgctcgc gcggctcccg gcggtggtgg 1920

dsRed1_C_pri ORF 3 ORF 3 DsRed2(variant) .......... ..........

.......... NotI(1932) .......... .......... .......... ..........

1921 tgttcctgta gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt 1980

1921 acaaggacat cgccggcgct gagatctagt attagtcggt atggtgtaaa catctccaaa 1980

ORF 3 DsRed2(variant) ORF 3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 2040

1981 atgaacgaaa ttttttggag ggtgtggagg gggacttgga ctttgtattt tacttacgtt 2040

.......... .......... .......... .......... .......... ..........

.......... HpaI(2051) .......... .......... .......... ..........

2041 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 2100

2041 aacaacaaca attgaacaaa taacgtcgaa tattaccaat gtttatttcg ttatcgtagt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 2160

2101 gtttaaagtg tttatttcgt aaaaaaagtg acgtaagatc aacaccaaac aggtttgagt 2160

.......... .......... .......... .......... ......... EBV_rev_pri

AflII(2170) ........ .......... .......... .......... ..........

2161 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 2220

2161 agttacatag aattccgcat ttaacattcg caattataaa acaattttaa gcgcaattta 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 2280

2221 aaaacaattt agtcgagtaa aaaattggtt atccggcttt agccgtttta gggaatattt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 2340

2281 agttttctta tctggctcta tcccaactca caacaaggtc aaaccttgtt ctcaggtgat 2340

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

2341 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 2400

2341 aatttcttgc acctgaggtt gcagtttccc gctttttggc agatagtccc gctaccgggt 2400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 2460

2401 gatgcacttg gtagtgggat tagttcaaaa aaccccagct ccacggcatt tcgtgattta 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 2520

2461 gccttgggat ttccctcggg ggctaaatct cgaactgccc ctttcggccg cttgcaccgc 2520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 2580

2521 tctttccttc ccttctttcg ctttcctcgc ccgcgatccc gcgaccgttc acatcgccag 2580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt 2640

2581 tgcgacgcgc attggtggtg tgggcggcgc gaattacgcg gcgatgtccc gcgcagtcca 2640

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 2700

2641 ccgtgaaaag cccctttaca cgcgccttgg ggataaacaa ataaaaagat ttatgtaagt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 2760

2701 ttatacatag gcgagtactc tgttattggg actatttacg aagttattat aactttttcc 2760

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt 2820

2761 ttctcaggac tccgcctttc ttggtcgaca ccttacacac agtcaatccc acacctttca 2820

.......... .......... .......... ..... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

2821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2880

2821 ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt 2880

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2940

2881 ccacaccttt caggggtccg aggggtcgtc cgtcttcata cgtttcgtac gtagagttaa 2940

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact c cgcccagtt 3000

2941 tcagtcgttg gtatcagggc ggggattgag gcgggtaggg cggggattga ggcgggtcaa 3000

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

3001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 3060

3001 ggcgggtaag aggcggggta ccgactgatt aaaaaaaata aatacgtctc cggctccggc 3060

SV40_enh ..... SV40_pro SV40_origin ... SV40pro_F_pri ........

.......... .......... .......... .......... .......... ..........

3061 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 3120

3061 ggagccggag actcgataag gtcttcatca ctcctccgaa aaaacctccg gatccgaaaa 3120

SV40_pro .......... .......... .......... .......... ..........

ClaI(3128) .......... .......... .......... .......... ..........

3121 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 3180

3121 cgtttctagc tagttctctg tcctactcct agcaaagcgt actaacttgt tctacctaac 3180

.......... .......... .......... .......... ORF 1 Neomycin

.......... .......... .......... .......... .......... ..........

3181 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 3240

3181 gtgcgtccaa gaggccggcg aacccacctc tccgataagc cgatactgac ccgtgttgtc 3240

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... NarI(3288) ..........

3241 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 3300

3241 tgttagccga cgagactacg gcggcacaag gccgacagtc gcgtccccgc gggccaagaa 3300

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 3360

3301 aaacagttct ggctggacag gccacgggac ttacttgacg ttctgctccg tcgcgccgat 3360

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 3420

3361 agcaccgacc ggtgctgccc gcaaggaacg cgtcgacacg agctgcaaca gtgacttcgc 3420

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 3480

3421 ccttccctga ccgacgataa cccgcttcac ggccccgtcc tagaggacag tagagtggaa 3480

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 3540

3481 cgaggacggc tctttcatag gtagtaccga ctacgttacg ccgccgacgt atgcgaacta 3540

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 3600

3541 ggccgatgga cgggtaagct ggtggttcgc tttgtagcgt agctcgctcg tgcatgagcc 3600

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 3660

3601 taccttcggc cagaacagct agtcctacta gacctgcttc tcgtagtccc cgagcgcggt 3660

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 3720

3661 cggcttgaca agcggtccga gttccgctcg tacgggctgc cgctcctaga gcagcactgg 3720

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 3780

3721 gtaccgctac ggacgaacgg cttatagtac caccttttac cggcgaaaag acctaagtag 3780

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 3840

3781 ctgacaccgg ccgacccaca ccgcctggcg atagtcctgt atcgcaaccg atgggcacta 3840

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 3900

3841 taacgacttc tcgaaccgcc gcttacccga ctggcgaagg agcacgaaat gccatagcgg 3900

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 3960

3901 cgagggctaa gcgtcgcgta gcggaagata gcggaagaac tgctcaagaa gactcgccct 3960

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 4020

3961 gagaccccaa gctttactgg ctggttcgct gcgggttgga cggtagtgct ctaaagctaa 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 4080

4021 ggtggcggcg gaagatactt tccaacccga agccttagca aaaggccctg cggccgacct 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa 4140

4081 actaggaggt cgcgccccta gagtacgacc tcaagaagcg ggtgggatcc ccctccgatt 4140

.......... .......... .......... .......... .......... TK_PA_term

.......... .......... .......... .......... .......... ..........

4141 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 4200

4141 gactttgtgc cttcctctgt tatggccttc cttgggcgcg atactgccgt tatttttctg 4200

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 4260

4201 tcttattttg cgtgccacaa cccagcaaac aagtatttgc gccccaagcc agggtcccga 4260

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 4320

4261 ccgtgagaca gctatggggt ggctctgggg taaccccggt tatgcgggcg caaagaagga 4320

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 4380

4321 aaaggggtgg ggtggggggt tcaagcccac ttccgggtcc cgagcgtcgg ttgcagcccc 4380

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc 4440

4381 gccgtccggg acggtatcgg agtccaatga gtatatatga aatctaacta aattttgaag 4440

TK_PA_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 4500

4441 taaaaattaa attttcctag atccacttct aggaaaaact attagagtac tggttttagg 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 4560

4501 gaattgcact caaaagcaag gtgactcgca gtctggggca tcttttctag tttcctagaa 4560

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4561 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 4620

4561 gaactctagg aaaaaaagac gcgcattaga cgacgaacgt ttgttttttt ggtggcgatg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 4680

4621 gtcgccacca aacaaacggc ctagttctcg atggttgaga aaaaggcttc cattgaccga 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 4740

4681 agtcgtctcg cgtctatggt ttatgacagg aagatcacat cggcatcaat ccggtggtga 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 4800

4741 agttcttgag acatcgtggc ggatgtatgg agcgagacga ttaggacaat ggtcaccgac 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 4860

4801 gacggtcacc gctattcagc acagaatggc ccaacctgag ttctgctatc aatggcctat 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 4920

4861 tccgcgtcgc cagcccgact tgccccccaa gcacgtgtgt cgggtcgaac ctcgcttgct 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 4980

4921 ggatgtggct tgactctatg gatgtcgcac tcgatactct ttcgcggtgc gaagggcttc 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 5040

4981 cctctttccg cctgtccata ggccattcgc cgtcccagcc ttgtcctctc gcgtgctccc 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 5100

5041 tcgaaggtcc ccctttgcgg accatagaaa tatcaggaca gcccaaagcg gtggagactg 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 5160

5101 aactcgcagc taaaaacact acgagcagtc cccccgcctc ggataccttt ttgcggtcgt 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 5220

5161 tgcgccggaa aaatgccaag gaccggaaaa cgaccggaaa acgagtgtac aagaaaggac 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat...... .......... 5264

5221 gcaatagggg actaagacac ctattggcat aatggcggta cgta...... .......... 5264

.......... .......... .......... .......... .......... ..........