Sequence of pIRES2-EGFP

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Sequence of pIRES2-EGFP

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Sequence of pIRES2-EGFP : 5308 BP; 1275 A; 1439 C; 1394 G; 1200 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI EcoRI PstI,SalI SacII SmaI,BamHI)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg 720

661 ctaggcgggg agagggaggg gggggggatt gcaatgaccg gcttcggcga accttattcc 720

.......... .......... .......... IRES .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag 780

721 ggccacacgc aaacagatat acaataaaag gtggtataac ggcagaaaac cgttacactc 780

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc 840

781 ccgggccttt ggaccgggac agaagaactg ctcgtaagga tccccagaaa ggggagagcg 840

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg 900

841 gtttccttac gttccagaca acttacagca cttccttcgt caaggagacc ttcgaagaac 900

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag 960

901 ttctgtttgt tgcagacatc gctgggaaac gtccgtcgcc ttggggggtg gaccgctgtc 960

.......... .......... IRES .......... .......... ..........

.......... .......... PmlI(983) .......... .......... ..........

961 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca 1020

961 cacggagacg ccggttttcg gtgcacatat tctatgtgga cgtttccgcc gtgttggggt 1020

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt 1080

1021 cacggtgcaa cactcaacct atcaacacct ttctcagttt accgagagga gttcgcataa 1080

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc 1140

1081 gttgttcccc gacttcctac gggtcttcca tggggtaaca taccctagac tagaccccgg 1140

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa 1200

1141 agccacgtgt acgaaatgta cacaaatcag ctccaatttt tttgcagatc cggggggctt 1200

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga 1260

1201 ggtgcccctg caccaaaagg aaactttttg tgctactatt ataccggtgt tggtaccact 1260

.......... .......... IRES .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1261 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 1320

1261 cgttcccgct cctcgacaag tggccccacc acgggtagga ccagctcgac ctgccgctgc 1320

.......... .......... ORF 3 EGFP .......... EGFP_N_pri ..........

.......... .......... .......... .......... .......... ..........

1321 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc 1380

1321 atttgccggt gttcaagtcg cacaggccgc tcccgctccc gctacggtgg atgccgttcg 1380

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 1440

1381 actgggactt caagtagacg tggtggccgt tcgacgggca cgggaccggg tgggagcact 1440

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg 1500

1441 ggtgggactg gatgccgcac gtcacgaagt cggcgatggg gctggtgtac ttcgtcgtgc 1500

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 1560

1501 tgaagaagtt caggcggtac gggcttccga tgcaggtcct cgcgtggtag aagaagttcc 1560

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc 1620

1561 tgctgccgtt gatgttctgg gcgcggctcc acttcaagct cccgctgtgg gaccacttgg 1620

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg 1680

1621 cgtagctcga cttcccgtag ctgaagttcc tcctgccgtt gtaggacccc gtgttcgacc 1680

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca 1740

1681 tcatgttgat gttgtcggtg ttgcagatat agtaccggct gttcgtcttc ttgccgtagt 1740

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact 1800

1741 tccacttgaa gttctaggcg gtgttgtagc tcctgccgtc gcacgtcgag cggctggtga 1800

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga 1860

1801 tggtcgtctt gtgggggtag ccgctgccgg ggcacgacga cgggctgttg gtgatggact 1860

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 1920

1861 cgtgggtcag gcgggactcg tttctggggt tgctcttcgc gctagtgtac caggacgacc 1920

.......... .......... ORF 3 EGFP .......... .......... EGFP_C_pri

.......... .......... .......... .......... .......... ..........

1921 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc 1980

1921 tcaagcactg gcggcggccc tagtgagagc cgtacctgct cgacatgttc atttcgccgg 1980

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 2040

1981 cgctgagatc tagtattagt cggtatggtg taaacatctc caaaatgaac gaaatttttt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(2095)

2041 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 2100

2041 ggagggtgtg gagggggact tggactttgt attttactta cgttaacaac aacaattgaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 2160

2101 caaataacgt cgaatattac caatgtttat ttcgttatcg tagtgtttaa agtgtttatt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... AflII(2214)

2161 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg 2220

2161 tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt gagtagttac atagaattcc 2220

.......... .......... ......... EBV_rev_pri ......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 2280

2221 gcatttaaca ttcgcaatta taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 2340

2281 gtaaaaaatt ggttatccgg ctttagccgt tttagggaat atttagtttt cttatctggc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 2400

2341 tctatcccaa ctcacaacaa ggtcaaacct tgttctcagg tgataatttc ttgcacctga 2400

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 2460

2401 ggttgcagtt tcccgctttt tggcagatag tcccgctacc gggtgatgca cttggtagtg 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 2520

2461 ggattagttc aaaaaacccc agctccacgg catttcgtga tttagccttg ggatttccct 2520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 2580

2521 cgggggctaa atctcgaact gcccctttcg gccgcttgca ccgctctttc cttcccttct 2580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 2640

2581 ttcgctttcc tcgcccgcga tcccgcgacc gttcacatcg ccagtgcgac gcgcattggt 2640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa 2700

2641 ggtgtgggcg gcgcgaatta cgcggcgatg tcccgcgcag tccaccgtga aaagcccctt 2700

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 2760

2701 tacacgcgcc ttggggataa acaaataaaa agatttatgt aagtttatac ataggcgagt 2760

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2761 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg 2820

2761 actctgttat tgggactatt tacgaagtta ttataacttt ttccttctca ggactccgcc 2820

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc 2880

2821 tttcttggtc gacaccttac acacagtcaa tcccacacct ttcaggggtc cgaggggtcg 2880

.......... ......... pBABE_3_pri SV40_enh SV40_pro .........

.......... .......... .......... .......... .......... ..........

2881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 2940

2881 tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtccacac ctttcagggg 2940

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 3000

2941 tccgaggggt cgtccgtctt catacgtttc gtacgtagag ttaatcagtc gttggtatca 3000

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 3060

3001 gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg taagaggcgg 3060

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

3061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct 3120

3061 ggtaccgact gattaaaaaa aataaatacg tctccggctc cggcggagcc ggagactcga 3120

....... SV40_origin SV40_pro SV40pro_F_pri ....... ..........

.......... .......... .......... StuI(3153) .......... ClaI(3172)

3121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag 3180

3121 taaggtcttc atcactcctc cgaaaaaacc tccggatccg aaaacgtttc tagctagttc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 3240

3181 tctgtcctac tcctagcaaa gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc 3240

.......... .......... .......... ........ ORF 3 Neomycin ........

.......... .......... .......... .......... .......... ..........

3241 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 3300

3241 ggcgaaccca cctctccgat aagccgatac tgacccgtgt tgtctgttag ccgacgagac 3300

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 3360

3301 tacggcggca caaggccgac agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg 3360

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 3420

3361 acaggccacg ggacttactt gacgttctgc tccgtcgcgc cgatagcacc gaccggtgct 3420

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... FspI(3434) .......... .......... .......... ..........

3421 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 3480

3421 gcccgcaagg aacgcgtcga cacgagctgc aacagtgact tcgcccttcc ctgaccgacg 3480

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 3540

3481 ataacccgct tcacggcccc gtcctagagg acagtagagt ggaacgagga cggctctttc 3540

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 3600

3541 ataggtagta ccgactacgt tacgccgccg acgtatgcga actaggccga tggacgggta 3600

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 3660

3601 agctggtggt tcgctttgta gcgtagctcg ctcgtgcatg agcctacctt cggccagaac 3660

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 3720

3661 agctagtcct actagacctg cttctcgtag tccccgagcg cggtcggctt gacaagcggt 3720

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 3780

3721 ccgagttccg ctcgtacggg ctgccgctcc tagagcagca ctgggtaccg ctacggacga 3780

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 3840

3781 acggcttata gtaccacctt ttaccggcga aaagacctaa gtagctgaca ccggccgacc 3840

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 3900

3841 cacaccgcct ggcgatagtc ctgtatcgca accgatgggc actataacga cttctcgaac 3900

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 3960

3901 cgccgcttac ccgactggcg aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg 3960

.......... ........ ORF 3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat 4020

3961 cgtagcggaa gatagcggaa gaactgctca agaagactcg ccctgagacc ccaagcttta 4020

........ ORF 3 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4080

4021 ctggctggtt cgctgcgggt tggacggtag tgctctaaag ctaaggtggc ggcggaagat 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4140

4081 actttccaac ccgaagcctt agcaaaaggc cctgcggccg acctactagg aggtcgcgcc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga 4200

4141 cctagagtac gacctcaaga agcgggtggg atccccctcc gattgacttt gtgccttcct 4200

.......... .......... .......... .......... TK_PA_term ..........

.......... .......... .......... .......... .......... ..........

4201 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg 4260

4201 ctgttatggc cttccttggg cgcgatactg ccgttatttt tctgtcttat tttgcgtgcc 4260

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac 4320

4261 acaacccagc aaacaagtat ttgcgcccca agccagggtc ccgaccgtga gacagctatg 4320

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc 4380

4321 gggtggctct ggggtaaccc cggttatgcg ggcgcaaaga aggaaaaggg gtggggtggg 4380

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat 4440

4381 gggttcaagc ccacttccgg gtcccgagcg tcggttgcag ccccgccgtc cgggacggta 4440

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 4500

4441 tcggagtcca atgagtatat atgaaatcta actaaatttt gaagtaaaaa ttaaattttc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 4560

4501 ctagatccac ttctaggaaa aactattaga gtactggttt tagggaattg cactcaaaag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4620

4561 caaggtgact cgcagtctgg ggcatctttt ctagtttcct agaagaactc taggaaaaaa 4620

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4680

4621 agacgcgcat tagacgacga acgtttgttt ttttggtggc gatggtcgcc accaaacaaa 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4740

4681 cggcctagtt ctcgatggtt gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4800

4741 tggtttatga caggaagatc acatcggcat caatccggtg gtgaagttct tgagacatcg 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4860

4801 tggcggatgt atggagcgag acgattagga caatggtcac cgacgacggt caccgctatt 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4920

4861 cagcacagaa tggcccaacc tgagttctgc tatcaatggc ctattccgcg tcgccagccc 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4980

4921 gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 5040

4981 tatggatgtc gcactcgata ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 5100

5041 cataggccat tcgccgtccc agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 5160

5101 gcggaccata gaaatatcag gacagcccaa agcggtggag actgaactcg cagctaaaaa 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5220

5161 cactacgagc agtccccccg cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 5280

5221 caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa ggacgcaata ggggactaag 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgtggataac cgtattaccg ccatgcat.. .......... .......... .......... 5308

5281 acacctattg gcataatggc ggtacgta.. .......... .......... .......... 5308

.......... .......... .......... .......... .......... ..........