Sequence of pIRES

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Sequence of pIRES

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Sequence of pIRES : 6092 BP; 1465 A; 1534 C; 1532 G; 1561 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60

1 agttataacc ggtaatcggt ataataagta accaatatat cgtatttagt tataaccgat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120

61 aaccggtaac gtatgcaaca tagatatagt attatacatg taaatataac cgagtacagg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SpeI(152) .......... ..........

121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180

121 ttatactggc ggtacaaccg taactaataa ctgatcaata attatcatta gttaatgccc 180

.......... .......... .......... .......... ....... CMV_immearly_pro

.......... .......... .......... .......... .......... ..........

181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg ta aatggccc 240

181 cagtaatcaa gtatcgggta tatacctcaa ggcgcaatgt attgaatgcc atttaccggg 240

.......... ....... CMV_immearly_pro ....... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300

241 cggaccgact ggcgggttgc tgggggcggg taactgcagt tattactgca tacaagggta 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360

301 tcattgcggt tatccctgaa aggtaactgc agttacccac ctcataaatg ccatttgacg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... NdeI(387) .......... .......... ..........

361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420

361 ggtgaaccgt catgtagttc acatagtata cggttcaggc gggggataac tgcagttact 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480

421 gccatttacc gggcggaccg taatacgggt catgtactgg aatgccctga aaggatgaac 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540

481 cgtcatgtag atgcataatc agtagcgata atggtaccac tacgccaaaa ccgtcatgtg 540

....... CAG_enh CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600

541 gttacccgca cctatcgcca aactgagtgc ccctaaaggt tcagaggtgg ggtaactgca 600

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg 660

601 gttaccctca aacaaaaccg tggttttagt tgccctgaaa ggttttacag cattgttgac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata 720

661 gctagcgggc ggggcaactg cgtttacccg ccatccgcac atgccaccct ccagatatat 720

.......... . CMV_immearly_pro CMV_fwd_pri CMV_pro . ..........

SacI(729) .......... .......... .......... .......... ..........

721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac 780

721 tcgtctcgag caaatcactt ggcagtctag tgatcttcga aataacgcca tcaaatagtg 780

CMV_pro pCEP_fwd_pri LNCX_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt 840

781 tcaatttaac gattgcgtca gtcacgaaga ctgtgttgtc agagcttgaa ttcgacgtca 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa 900

841 ctgagagaat tccatcggaa cgtcttcaac cagcactccg tgacccgtcc attcatagtt 900

.......... .......... .......... .......... .......... chim_int

.......... .......... .......... .......... .......... ..........

901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact 960

901 ccaatgttct gtccaaattc ctctggttat ctttgacccg aacagctctg tctcttctga 960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac 1020

961 gaacgcaaag actatccgtg gataaccaga atgactgtag gtgaaacgga aagagaggtg 1020

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact 1080

1021 tccacaggtg agggtcaagt taatgtcgag aattccgatc tcatgaatta tgctgagtga 1080

.......... .......... .......... .......... .......... T7_pro

NheI(1085) XhoI(1091),EcoRI(1096) ........ .......... ..........

1081 ataggctagc ctcgagaatt cacgcgtcga gcatgcatct agggcggcca attccgcccc 1140

1081 tatccgatcg gagctcttaa gtgcgcagct cgtacgtaga tcccgccggt taaggcgggg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg 1200

1141 agagggaggg gggggggatt gcaatgaccg gcttcggcga accttattcc ggccacacgc 1200

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(1254)

1201 tttgtctata tgtgattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa 1260

1201 aaacagatat acactaaaag gtggtataac ggcagaaaac cgttacactc ccgggccttt 1260

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg 1320

1261 ggaccgggac agaagaactg ctcgtaagga tccccagaaa ggggagagcg gtttccttac 1320

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca 1380

1321 gttccagaca acttacagca cttccttcgt caaggagacc ttcgaagaac ttctgtttgt 1380

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc 1440

1381 tgcagacatc gctgggaaac gtccgtcgcc ttggggggtg gaccgctgtc cacggagacg 1440

.......... .......... IRES .......... .......... ..........

.......... PmlI(1453) .......... .......... .......... ..........

1441 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt 1500

1441 ccggttttcg gtgcacatat tctatgtgga cgtttccgcc gtgttggggt cacggtgcaa 1500

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg 1560

1501 cactcaacct atcaacacct ttctcagttt accgagagga gttcgcataa gttgttcccc 1560

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca 1620

1561 gacttcctac gggtcttcca tggggtaaca taccctagac tagaccccgg agccacgtgt 1620

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac 1680

1621 acgaaatgta cacaaatcag ctccaatttt tttgcagatc cggggggctt ggtgcccctg 1680

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... XbaI(1732)

1681 gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccgggatc ctctagagtc 1740

1681 caccaaaagg aaactttttg tgctactatt cgaacggtgt tgggccctag gagatctcag 1740

IRES .......... .......... .......... .......... ..........

NotI(1749) .......... .......... .......... .......... ..........

1741 gacccgggcg gccgcttccc tttagtgagg gttaatgctt cgagcagaca tgataagata 1800

1741 ctgggcccgc cggcgaaggg aaatcactcc caattacgaa gctcgtctgt actattctat 1800

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

1801 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 1860

1801 gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact 1860

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... HpaI(1915)

1861 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 1920

1861 ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt 1920

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag 1980

1921 gttgttaacg taagtaaaat acaaagtcca agtccccctc tacaccctcc aaaaaatttc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 caagtaaaac ctctacaaat gtggtaaaat ccgataagga tcgatccggg ctggcgtaat 2040

1981 gttcattttg gagatgttta caccatttta ggctattcct agctaggccc gaccgcatta 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 2100

2041 tcgcttctcc gggcgtggct agcgggaagg gttgtcaacg cgtcggactt accgcttacc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 2160

2101 tgcgcgggac atcgccgcgt aattcgcgcc gcccacacca ccaatgcgcg tcgcactggc 2160

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 2220

2161 gatgtgaacg gtcgcgggat cgcgggcgag gaaagcgaaa gaagggaagg aaagagcggt 2220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 2280

2221 gcaagcggcc gaaaggggca gttcgagatt tagcccccga gggaaatccc aaggctaaat 2280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gagctttacg gcacctcgac cgcaaaaaac ttgatttggg tgatggttca cgtagtgggc 2340

2281 ctcgaaatgc cgtggagctg gcgttttttg aactaaaccc actaccaagt gcatcacccg 2340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 2400

2341 gtagcgggac tatctgccaa aaagcgggaa actgcaacct caggtgcaag aaattatcac 2400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 2460

2401 ctgagaacaa ggtttgacct tgttgtgagt tgggatagag ccagataaga aaactaaata 2460

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaatattta 2520

2461 ttccctaaaa cggctaaagc cggataacca attttttact cgactaaatt gtttataaat 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acgcgaattt taacaaaata ttaacgttta caatttcgcc tgatgcggta ttttctcctt 2580

2521 tgcgcttaaa attgttttat aattgcaaat gttaaagcgg actacgccat aaaagaggaa 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa 2640

2581 tgcgtagaca cgccataaag tgtggcgtat gcgcctagac gcgtcgtggt accggacttt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga 2700

2641 attggagact ttctccttga accaatccat ggaagactcc gcctttcttg gtcgacacct 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 2760

2701 tacacacagt caatcccaca cctttcaggg gtccgagggg tcgtccgtct tcatacgttt 2760

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2761 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 2820

2761 cgtacgtaga gttaatcagt cgttggtcca cacctttcag gggtccgagg ggtcgtccgt 2820

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 2880

2821 cttcatacgt ttcgtacgta gagttaatca gtcgttggta tcagggcggg gattgaggcg 2880

.......... ...... SV40_enh SV40_pro SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

2881 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 2940

2881 ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg cggggtaccg actgattaaa 2940

..... SV40_enh SV40_pro SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2941 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 3000

2941 aaaaataaat acgtctccgg ctccggcgga gccggagact cgataaggtc ttcatcactc 3000

SV40_origin SV40pro_F_pri SV40_pro SV40_pro ...... ..........

.......... StuI(3016) .......... .......... .......... ..........

3001 gaggcttttt tggaggccta ggcttttgca aaaagcttga ttcttctgac acaacagtct 3060

3001 ctccgaaaaa acctccggat ccgaaaacgt ttttcgaact aagaagactg tgttgtcaga 3060

.......... SV40_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgaacttaag gctagagcca ccatgattga acaagatgga ttgcacgcag gttctccggc 3120

3061 gcttgaattc cgatctcggt ggtactaact tgttctacct aacgtgcgtc caagaggccg 3120

.......... .......... .......... ........ ORF 2 Neomycin ........

.......... .......... .......... .......... .......... ..........

3121 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 3180

3121 gcgaacccac ctctccgata agccgatact gacccgtgtt gtctgttagc cgacgagact 3180

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... NarI(3211) .......... ..........

3181 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 3240

3181 acggcggcac aaggccgaca gtcgcgtccc cgcgggccaa gaaaaacagt tctggctgga 3240

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 3300

3241 caggccacgg gacttacttg acgtcctgct ccgtcgcgcc gatagcaccg accggtgctg 3300

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 3360

3301 cccgcaagga acgcgtcgac acgagctgca acagtgactt cgcccttccc tgaccgacga 3360

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 3420

3361 taacccgctt cacggccccg tcctagagga cagtagagtg gaacgaggac ggctctttca 3420

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 3480

3421 taggtagtac cgactacgtt acgccgccga cgtatgcgaa ctaggccgat ggacgggtaa 3480

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 3540

3481 gctggtggtt cgctttgtag cgtagctcgc tcgtgcatga gcctaccttc ggccagaaca 3540

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 3600

3541 gctagtccta ctagacctgc ttctcgtagt ccccgagcgc ggtcggcttg acaagcggtc 3600

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 3660

3601 cgagttccgc gcgtacgggc tgccgctcct agagcagcac tgggtaccgc tacggacgaa 3660

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 3720

3661 cggcttatag taccaccttt taccggcgaa aagacctaag tagctgacac cggccgaccc 3720

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 3780

3721 acaccgcctg gcgatagtcc tgtatcgcaa ccgatgggca ctataacgac ttctcgaacc 3780

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 3840

3781 gccgcttacc cgactggcga aggagcacga aatgccatag cggcgagggc taagcgtcgc 3840

.......... ........ ORF 2 Neomycin ........ .......... ..........

.......... .......... .......... .......... ......... BstBI(3893)

3841 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 3900

3841 gtagcggaag atagcggaag aactgctcaa gaagactcgc cctgagaccc caagctttac 3900

........ ORF 2 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accgaccaag cgacgcccaa cctgccatca cgatggccgc aataaaatat ctttattttc 3960

3901 tggctggttc gctgcgggtt ggacggtagt gctaccggcg ttattttata gaaataaaag 3960

.......... .......... .......... ........ synth_PA_term ........

.......... .......... .......... .......... .......... ..........

3961 attacatctg tgtgttggtt ttttgtgtga atcgatagcg ataaggatcc gcgtatggtg 4020

3961 taatgtagac acacaaccaa aaaacacact tagctatcgc tattcctagg cgcataccac 4020

........ synth_PA_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 4080

4021 gtgagagtca tgttagacga gactacggcg tatcaattcg gtcggggctg tgggcggttg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 4140

4081 tgggcgactg cgcgggactg cccgaacaga cgagggccgt aggcgaatgt ctgttcgaca 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 4200

4141 ctggcagagg ccctcgacgt acacagtctc caaaagtggc agtagtggct ttgcgcgctc 4200

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 4260

4201 tgctttcccg gagcactatg cggataaaaa tatccaatta cagtactatt attaccaaag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 4320

4261 aatctgcagt ccaccgtgaa aagccccttt acacgcgcct tggggataaa caaataaaaa 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 4380

4321 gatttatgta agtttataca taggcgagta ctctgttatt gggactattt acgaagttat 4380

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 4440

4381 tataactttt tccttctcat actcataagt tgtaaaggca cagcgggaat aagggaaaaa 4440

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4441 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 4500

4441 acgccgtaaa acggaaggac aaaaacgagt gggtctttgc gaccactttc attttctacg 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 4560

4501 acttctagtc aacccacgtg ctcacccaat gtagcttgac ctagagttgt cgccattcta 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 4620

4561 ggaactctca aaagcggggc ttcttgcaaa aggttactac tcgtgaaaat ttcaagacga 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 4680

4621 tacaccgcgc cataataggg cataactgcg gcccgttctc gttgagccag cggcgtatgt 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 4740

4681 gataagagtc ttactgaacc aactcatgag tggtcagtgt cttttcgtag aatgcctacc 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 4800

4741 gtactgtcat tctcttaata cgtcacgacg gtattggtac tcactattgt gacgccggtt 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 4860

4801 gaatgaagac tgttgctagc ctcctggctt cctcgattgg cgaaaaaacg tgttgtaccc 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 4920

4861 cctagtacat tgagcggaac tagcaaccct tggcctcgac ttacttcggt atggtttgct 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 4980

4921 gctcgcactg tggtgctacg gacatcgtta ccgttgttgc aacgcgtttg ataattgacc 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 5040

4981 gcttgatgaa tgagatcgaa gggccgttgt taattatctg acctacctcc gcctatttca 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 5100

5041 acgtcctggt gaagacgcga gccgggaagg ccgaccgacc aaataacgac tatttagacc 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 5160

5101 tcggccactc gcacccagag cgccatagta acgtcgtgac cccggtctac cattcgggag 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 5220

5161 ggcatagcat caatagatgt gctgcccctc agtccgttga tacctacttg ctttatctgt 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 5280

5221 ctagcgactc tatccacgga gtgactaatt cgtaaccatt gacagtctgg ttcaaatgag 5280

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 5340

5281 tatatatgaa atctaactaa attttgaagt aaaaattaaa ttttcctaga tccacttcta 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 5400

5341 ggaaaaacta ttagagtact ggttttaggg aattgcactc aaaagcaagg tgactcgcag 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 5460

5401 tctggggcat cttttctagt ttcctagaag aactctagga aaaaaagacg cgcattagac 5460

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

5461 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 5520

5461 gacgaacgtt tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc tagttctcga 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 5580

5521 tggttgagaa aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt tatgacagga 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 5640

5581 agatcacatc ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg gatgtatgga 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 5700

5641 gcgagacgat taggacaatg gtcaccgacg acggtcaccg ctattcagca cagaatggcc 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 5760

5701 caacctgagt tctgctatca atggcctatt ccgcgtcgcc agcccgactt gccccccaag 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 5820

5761 cacgtgtgtc gggtcgaacc tcgcttgctg gatgtggctt gactctatgg atgtcgcact 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 5880

5821 cgatactctt tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag gccattcgcc 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 5940

5881 gtcccagcct tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga ccatagaaat 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 6000

5941 atcaggacag cccaaagcgg tggagactga actcgcagct aaaaacacta cgagcagtcc 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 6060

6001 ccccgcctcg gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac 6060

........ pBR322_origin ........ .......... .......... ..........

.......... ......... BglII(6087) ......... .......... ..........

6061 ctggcctttt gctcacatgg ctcgacagat ct........ .......... .......... 6092

6061 gaccggaaaa cgagtgtacc gagctgtcta ga........ .......... .......... 6092

.......... .......... .......... .......... .......... ..........