q=q=D$LOCUS pIRESbleo3 4874 bp DNA SYN 28-Sep-2007 DEFINITION pIRESbleo3 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4874 /organism="pIRESbleo3" /mol_type="other DNA" misc_feature 315..602 /label="CAG_enhancer" promoter 236..852 /label="CMV_immearly_promoter" misc_feature 769..789 /label="CMV_fwd_primer" promoter 863..881 /label="T7_promoter" misc_feature 974..1269 /label="synth_int" misc_feature 1296..1859 /label="IRES" CDS 1780..2385 /label="bleo" /gene="bleo" /note="ORF frame 1" /translation="MLYMCLVEVKKTSRPPEPRGRGFPLKNTMISLPQPSLPAGAPSG KVGKPCRSDHHGQVDQCRSGAHRARRRRSGRVLDRPARVLPGLRGGRLRRCGPGRRDP VHQRGPGPGGAGQHPGLGVGARPGRAVRRVVGGRVHELPGRLRAGHDRDRRAAVGAGV RPARPGRQLRALRGRGAGLTDADQHRRSDAARRVRGIVDSR*" CDS complement(1795..2403) /label="ORF frame 1" /translation="MADYDQLSRVDDASDPSGRVGPAVLVGVGQSCSSATKCTQLPAG SRRANSRPHGCSPISVMAGPEASRKFVDTTSDHSAYSSSRPRTHTQARVLSGTTWSWT ALMNRVTSSRTTPAKSSSTKSRENPSRSVQNSTAPATSRAVSTGTALVNLAMVIRSAG LPNLTRGRPSWQAGLWQAYHRVFQRKTTSPWFGGPRRFFNLD*" gene 1943..2314 /label="bleo" /gene="bleo" misc_feature 1943..2317 /label="sh_ble" terminator 2387..2615 /label="SV40_PA_terminator" misc_feature 2585..2604 /label="EBV_rev_primer" misc_feature 2638..2657 /label="EBV_rev_primer" misc_feature complement(2713..2731) /label="M13_reverse_primer" misc_feature complement(2730..2752) /label="M13_pUC_rev_primer" promoter complement(2766..2795) /label="lac_promoter" rep_origin complement(3104..3723) /label="pBR322_origin" CDS complement(3878..4738) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3878..4738) /label="Ampicillin" /gene="Ampicillin" promoter complement(4780..4808) /label="AmpR_promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat cgatatctgc ggccgcgtcg acggaattca gtggatccac tagtaacggc 961 cgccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc 1021 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat 1081 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa 1141 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact 1201 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac 1261 tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa 1321 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 1381 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 1441 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 1501 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 1561 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 1621 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 1681 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 1741 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 1801 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 1861 acgatgataa gcttgccaca acccagcttg ccagctgggg cgccctctgg taaggttggg 1921 aagccctgca gatctgatca ccatggccaa gttgaccagt gccgttccgg tgctcaccgc 1981 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 2041 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 2101 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 2161 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 2221 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 2281 caactgcgtg cacttcgtgg ccgaggagca ggactgaccg acgccgacca acaccgccgg 2341 tccgacgcgg cccgacgggt ccgaggcatc gtcgactcta gataactgat cataatcagc 2401 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 2461 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 2521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 2581 agttgtggtt tgtccaaact catcaatgta tcttaacgcg tcgagtgcat tctagttgtg 2641 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 2701 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 2761 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 2821 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 2881 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 2941 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3001 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3061 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3121 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3181 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3241 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 3301 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3361 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3421 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3481 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3541 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 3601 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3661 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3721 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3781 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 3841 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 3901 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3961 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 4021 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 4081 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 4141 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 4201 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 4261 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 4321 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 4381 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 4441 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 4501 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4561 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 4621 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 4681 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 4741 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4801 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4861 tgccacctga cgtc //$ pIRESbleo3 "Times New Roman q=C$;ZCAG_enh"Times New Romanq=C$TCMV_immearly_pro"Times New Romanq=CɆ$ CMV_fwd_pri"Times New Romanq=$_qT7_pro"Times New Romanq=C$ synth_int"Times New Romanq=C$CIRES"Times New Romanq=CɆ$Q ORF 1"Times New Romanq=$c ORF 1"Times New Roman*q=C$ bleo"Times New Romanq=C$ sh_ble"Times New Roman?q=CɆ$S 7  SV40_PA_term"Times New Romanq=$ ,  EBV_rev_pri"Times New Romanq=C$N a  EBV_rev_pri"Times New Romanq=C$ M13_reverse_pri"Times New Romanq=CɆ$ M13_pUC_rev_pri"Times New Romanq=$ lac_pro"Times New Romanq=C$  pBR322_origin"Times New Romanq=C$&ORF 2"Times New Romanq=CɆ$& Ampicillin"Times New Romanq=$AmpR_pro"Times New Roman q=NruI"Times New Roman$xoeVe q=NdeI"Times New Roman$7=BBz| q=4ClaI(910),EcoRV(914),NotI(920),EcoRI(934),BamHI(943)"Times New Roman$ q=SacII"Times New RomanL$/-+$2 q=ApaI"Times New Roman$ q=PmlI"Times New RomanM$ q=NarI(1900),SmaI(2033)"Times New Romanl$JQ WW1 q=XbaI(2377),HpaI(2493)"Times New RomanI $;ENN q=FspI"Times New RomanM$ q=test"Times New Roman.$G.B q=test"Times New Roman0$##23D3D| q=test"Times New RomanP $7~@kKKk