Sequence of pIRESbleo3

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Sequence of pIRESbleo3

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Sequence of pIRESbleo3 : 4874 BP; 1158 A; 1263 C; 1237 G; 1216 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 60

1 ctgcctagcc ctctagaggg ctaggggata ccagctgaga gtcatgttag acgagactac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120

61 ggcgtatcaa ttcggtcata gacgagggac gaacacacaa cctccagcga ctcatcacgc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180

121 gctcgtttta aattcgatgt tgttccgttc cgaactggct gttaacgtac ttcttagacg 180

.......... .......... .......... .......... .......... ..........

.......... .......... NruI(208) .......... .......... ..........

181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240

181 aatcccaatc cgcaaaacgc gacgaagcgc tacatgcccg gtctatatgc gcaactgtaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 300

241 ctaataactg atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat 300

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 360

301 acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg 360

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 420

361 gggcgggtaa ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg 420

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 480

421 taactgcagt tacccacctg ataaatgcca tttgacgggt gaaccgtcat gtagttcaca 480

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

NdeI(484) .......... .......... .......... .......... ..........

481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 540

481 tagtatacgg ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa 540

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 600

541 tacgggtcat gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt 600

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 660

601 agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac 660

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 720

661 tgagtgcccc taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg 720

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 780

721 ttttagttgc cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc 780

.......... ....... CMV_immearly_pro ....... ......... CMV_fwd_pri

.......... .......... .......... .......... .......... ..........

781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 840

781 catccgcaca tgccaccctc cagatatatt cgtctcgaga gaccgattga tctcttgggt 840

CMV_fwd_pri ..... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 900

841 gacgaatgac cgaatagctt taattatgct gagtgatatc cctctgggtt cgaaccatgg 900

CMV_immearly_pro .... .......... T7_pro .......... ..........

ClaI0),EcoRV(914) NotI(920) EcoRI(934) BamHI(943) ..........

901 gagctcggat cgatatctgc ggccgcgtcg acggaattca gtggatccac tagtaacggc 960

901 ctcgagccta gctatagacg ccggcgcagc tgccttaagt cacctaggtg atcattgccg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg tgagtactcc 1020

961 gcggtcacac gaccttaatt aagcgacaga cgctcccggt cgacaacccc actcatgagg 1020

.......... .......... .......... synth_int .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat 1080

1021 gagagttttc gcccgtactg aagacgcgat tctaacagtc aaaggttttt gctcctccta 1080

.......... .......... synth_int .......... .......... ..........

......... SacII(1100) ......... .......... .......... ..........

1081 ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat ctggtcagaa 1140

1081 aactataagt ggaccgggcg ccactacgga aactcccacc ggcgcaggta gaccagtctt 1140

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg gccatacact 1200

1141 ttctgttaga aaaacaacag ttcgaactcc acaccgtccg aactctagac cggtatgtga 1200

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac 1260

1201 actcactgtt actgtaggtg aaacggaaag agaggtgtcc acaggtgagg gtccaggttg 1260

.......... .......... synth_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccctccc ccccccctaa 1320

1261 acgtccagct cgtacgtaga tcccgccggt taaggcgggg agagggaggg gggggggatt 1320

synth_int .......... .......... .......... IRES ..........

.......... .......... .......... .......... .......... ..........

1321 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 1380

1321 gcaatgaccg gcttcggcga accttattcc ggccacacgc aaacagatat acaataaaag 1380

.......... .......... IRES .......... .......... ..........

.......... .......... .......... ApaI(1414) .......... ..........

1381 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 1440

1381 gtggtataac ggcagaaaac cgttacactc ccgggccttt ggaccgggac agaagaactg 1440

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 1500

1441 ctcgtaagga tccccagaaa ggggagagcg gtttccttac gttccagaca acttacagca 1500

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 1560

1501 cttccttcgt caaggagacc ttcgaagaac ttctgtttgt tgcagacatc gctgggaaac 1560

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(1613)

1561 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 1620

1561 gtccgtcgcc ttggggggtg gaccgctgtc cacggagacg ccggttttcg gtgcacatat 1620

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 1680

1621 tctatgtgga cgtttccgcc gtgttggggt cacggtgcaa cactcaacct atcaacacct 1680

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 1740

1681 ttctcagttt accgagagga gttcgcataa gttgttcccc gacttcctac gggtcttcca 1740

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat g tgtttagtc 1800

1741 tggggtaaca taccctagac tagaccccgg agccacgtgt acgaaatgta cacaaatcag 1800

.......... .......... IRES .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1801 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 1860

1801 ctccaatttt tttgcagatc cggggggctt ggtgcccctg caccaaaagg aaactttttg 1860

.......... ....... IRES ORF 1 ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acgatgataa gcttgccaca acccagcttg ccagctgggg cgccctctgg taaggttggg 1920

1861 tgctactatt cgaacggtgt tgggtcgaac ggtcgacccc gcgggagacc attccaaccc 1920

.......... ......... ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aagccctgca gatctgatca ccatggccaa gttgaccagt gccgt tccgg tgctcaccgc 1980

1921 ttcgggacgt ctagactagt ggtaccggtt caactggtca cggcaaggcc acgagtggcg 1980

.......... ......... ORF 1 ORF 1 ........ bleo sh_ble .........

.......... .......... .......... .......... .......... SmaI(2033)

1981 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt 2040

1981 cgcgctgcag cggcctcgcc agctcaagac ctggctggcc gagcccaaga gggccctgaa 2040

.......... ... ORF 1 ORF 1 bleo sh_ble ... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt 2100

2041 gcacctcctg ctgaagcggc cacaccaggc cctgctgcac tgggacaagt agtcgcgcca 2100

.......... ... ORF 1 ORF 1 bleo sh_ble ... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga 2160

2101 ggtcctggtc caccacggcc tgttgtggga ccggacccac acccacgcgc cggacctgct 2160

.......... ... ORF 1 ORF 1 bleo sh_ble ... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc 2220

2161 cgacatgcgg ctcaccagcc tccagcacag gtgcttgaag gccctgcgga ggcccggccg 2220

.......... ... ORF 1 ORF 1 bleo sh_ble ... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg 2280

2221 gtactggctc tagccgctcg tcggcacccc cgccctcaag cgggacgcgc tgggccggcc 2280

.......... ... ORF 1 ORF 1 bleo sh_ble ... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 caactgcgtg cacttcgtgg ccgaggagca ggactgaccg acgccgacca acaccgccgg 2340

2281 gttgacgcac gtgaagcacc ggctcctcgt cctgactggc tgcggctggt tgtggcggcc 2340

........ bleo sh_ble ORF 1 ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccgacgcgg cccgacgggt ccgaggcatc gtcgactcta gataactgat cataatcagc 2400

2341 aggctgcgcc gggctgccca ggctccgtag cagctgagat ctattgacta gtattagtcg 2400

.......... ......... ORF 1 ORF 1 ......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2401 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 2460

2401 gtatggtgta aacatctcca aaatgaacga aattttttgg agggtgtgga gggggacttg 2460

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... HpaI(2493) .......... ..........

2461 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 2520

2461 gactttgtat tttacttacg ttaacaacaa caattgaaca aataacgtcg aatattacca 2520

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 2580

2521 atgtttattt cgttatcgta gtgtttaaag tgtttatttc gtaaaaaaag tgacgtaaga 2580

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agttgtggtt tgtccaaact catcaatgta tcttaacgcg tcgagtgcat tctagttgtg 2640

2581 tcaacaccaa acaggtttga gtagttacat agaattgcgc agctcacgta agatcaacac 2640

... SV40_PA_term EBV_rev_pri ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 2700

2641 caaacaggtt tgagtagtta catagaatag tacagacata tggcagctgg agatcgatct 2700

EBV_rev_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 2760

2701 cgaaccgcat tagtaccagt atcgacaaag gacacacttt aacaataggc gagtgttaag 2760

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

2761 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 2820

2761 gtgtgttgta tgctcggcct tcgtatttca catttcggac cccacggatt actcactcga 2820

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 2880

2821 ttgagtgtaa ttaacgcaac gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 2940

2881 tcgacgtaat tacttagccg gttgcgcgcc cctctccgcc aaacgcataa cccgcgagaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 3000

2941 ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct cgccatagtc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 3060

3001 gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt cctttcttgt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 3120

3061 acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac gaccgcaaaa 3120

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3121 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 3180

3121 aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca gtctccaccg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 3240

3181 ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg gagcacgcga 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 3300

3241 gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga agcccttcgc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3360

3301 accgcgaaag agttacgagt gcgacatcca tagagtcaag ccacatccag caagcgaggt 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3420

3361 tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat aggccattga 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3480

3421 tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt cggtgaccat 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3540

3481 tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc accaccggat 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 3600

3541 tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc ggtcaatgga 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3660

3601 agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca tcgccaccaa 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3720

3661 aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt ctaggaaact 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3780

3721 agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc taaaaccagt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 3840

3781 actctaatag tttttcctag aagtggatct aggaaaattt aatttttact tcaaaattta 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 3900

3841 gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat tagtcactcc 3900

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3901 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3960

3901 gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag gggcagcaca 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 4020

3961 tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac tatggcgctc 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 4080

4021 tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct tcccggctcg 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 4140

4081 cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca acggcccttc 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(4173) .......... ..........

4141 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 4200

4141 gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa cgatgtccgt 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 4260

4201 agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg gttgctagtt 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 4320

4261 ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag ccaggaggct 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 4380

4321 agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt cgtgacgtat 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 4440

4381 taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc atgagttggt 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 4500

4441 tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc agttatgccc 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4560

4501 tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt gcaagaagcc 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 4620

4561 ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt gggtgagcac 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 4680

4621 gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact cgtttttgtc 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 4740

4681 cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact tatgagtatg 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4800

4741 agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac tcgcctatgt 4800

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4801 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4860

4801 ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgccacctga cgtc...... .......... .......... .......... .......... 4874

4861 acggtggact gcag...... .......... .......... .......... .......... 4874

.......... .......... .......... .......... .......... ..........